Cell Segmentation: Generation of a Cell Mask for Time Lapse Movies
2:33
Loading the Generated Cell Mask into Surface Manager
3:36
Analysis of Actomyosin Pulses in Surface Manager at Local Cellular Scale
4:51
Selective Surface Extraction of Curved Epithelial Tissues
8:41
Results: Behavior of Myosin II at the Cellular and Tissue Scale in a Drosophila Egg Chamber
10:13
Conclusion
Transcrição
Our protocol helps scientists to analyze subcellular actomyosin network in individual epithelial cells and also allows them to perform similar analysis at multicellular scale in curved epithelial tissues. Our protocol is a nice example of a Fiji a
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This protocol provides a Fiji-based, user-friendly methodology along with straightforward instructions explaining how to reliably analyze actomyosin behavior in individual cells and curved epithelial tissues. No programming skills are required to follow the tutorial; all steps are performed in a semi-interactive manner using the graphical user interface of Fiji and associated plugins.