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Nagoya University Institute for Advanced Research

2 ARTICLES PUBLISHED IN JoVE

Bioengineering

Selective Cell Elimination from Mixed 3D Culture Using a Near Infrared Photoimmunotherapy Technique

Kazuhide Sato¹, Peter L. Choyke¹, Kobayashi Hisataka¹

¹Molecular Imaging Program, National Cancer Institute

Eliminating specific cells without damaging other cells is extremely difficult, especially in established tissue, yet there is an urgent need for a cell elimination method in the tissue engineering field. Here, we present a method for specific cell elimination from a mixed 3D cell culture using near infrared photoimmunotherapy (NIR-PIT).

Medicine

Near Infrared Photoimmunotherapy for Mouse Models of Pleural Dissemination

Hirotoshi Yasui¹, Yuko Nishinaga¹, Shunichi Taki¹, Kazuomi Takahashi¹, Yoshitaka Isobe¹, Kazuhide Sato^1,2,3

¹Respiratory Medicine, Nagoya University Graduate School of Medicine, ²Nagoya University Institute for Advanced Research, ³B3-Unit, Advanced Analytical and Diagnostic Imaging Center (AADIC)/Medical Engineering Unit (MEU), Nagoya University Institute for Advanced Research

Near-infrared photoimmunotherapy (NIR-PIT) is an emerging cancer therapeutic strategy that utilizes an antibody-photoabsorber (IR700Dye) conjugate and NIR light to destroy cancer cells. Here, we present a method to evaluate the antitumor effect of NIR-PIT in a mouse model of pleural disseminated lung cancer and malignant pleural mesothelioma using bioluminescence imaging.