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Beijing Tongren Hospital, Capital Medical University, Beijing Ophthalmology & Visual Science Key Laboratory

3 ARTICLES PUBLISHED IN JoVE

Neuroscience

An Improved Method for Collection of Cerebrospinal Fluid from Anesthetized Mice

Nastasia K-H Lim^1,2, Visse Moestrup^3,4, Xiao Zhang¹, Wen-An Wang⁵, Arne Møller^3,4,6, Fu-De Huang^1,4

¹Shanghai Advanced Research Institute, University of Chinese Academy of Sciences, Chinese Academy of Science, ²Shanghai Institute of Materia Medica, University of Chinese Academy of Sciences, Chinese Academy of Science, ³Center of Functionally Integrative Neuroscience, Department of Clinical Medicine, Aarhus University, ⁴Sino-Danish Center for Education and Research (SDC), ⁵Department of Neurology, Xinhua Hospital Chongming Branch Affiliated to Shanghai Jiao Tong University School of Medicine, ⁶Department of Nuclear Medicine and PET-centre, Aarhus University Hospital

This protocol describes an improved technique for the abundant collection of cerebrospinal fluid (CSF) with no contamination from blood. With greater sample collection and purity, more analyses can be performed using CSF to further our understanding of diseases that affect the brain and spinal cord.

Medicine

Directed Induction of Retinal Organoids from Human Pluripotent Stem Cells

Xiao Zhang¹, Zi-Bing Jin¹

¹Beijing Institute of Ophthalmology, Beijing Tongren Eye Center, Beijing Tongren Hospital, Capital Medical University, Beijing Ophthalmology & Visual Science Key Laboratory

Using a self-organizing method, we develop a protocol with the addition of COCO that could significantly increase the generation of photoreceptors.

Cancer Research

Reconstruct Human Retinoblastoma In Vitro

Xiao Zhang¹, Zi-Bing Jin¹

¹Beijing Institute of Ophthalmology, Beijing Tongren Eye Center, Beijing Tongren Hospital, Capital Medical University, Beijing Ophthalmology & Visual Science Key Laboratory

We describe a method for generating human retinoblastoma (RB) by introducing biallelic RB1 mutations in human embryonic stem cells (hESC). RB cell lines could also be successfully cultured using the isolated RB in a dish.