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The Chinese University of Hong Kong, Prince of Wales Hospital

2 ARTICLES PUBLISHED IN JoVE

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Medicine

Monitoring of Systemic and Hepatic Hemodynamic Parameters in Mice
Chichi Xie 1,3, Weiwei Wei 1, Tao Zhang 1, Olaf Dirsch 2, Uta Dahmen 1
1Department of General, Visceral and Vascular Surgery, Jena University Hospital, 2Institute of Pathology, Jena University Hospital, 3Reproductive Medicine Center, The First Affiliated Hospital of Wenzhou Medical University

This film demonstrates how to acquire systemic and hepatic hemodynamics in mice. The whole monitoring includes acquisition of vital parameters, systemic blood pressure, central venous pressure, common hepatic artery flow rate, and portal vein pressure as well as the portal flow rate in mice.

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JoVE Core

Multiplexed Fluorescent Immunohistochemical Staining of Four Endometrial Immune Cell Types in Recurrent Miscarriage
Yiwei Zhao 1, Gene Chi Wai Man 1,2, Loucia Kit Ying Chan 1, Xi Guo 1, Yingyu Liu 3, Tao Zhang 1, Joseph Kwong 1,4, Chi Chiu Wang 1,5, Xiaoyan Chen 3, Tin Chiu Li 1
1Department of Obstetrics and Gynaecology, The Chinese University of Hong Kong, Prince of Wales Hospital, 2Department of Orthopaedics and Traumatology, The Chinese University of Hong Kong, Prince of Wales Hospital, 3Department of Obstetrics and Gynaecology, Shenzhen Baoan Women’s and Children’s Hospital, Shenzhen University, 4School of Medicine, Faculty of Medicine and Health Sciences, Keele University, 5Li Ka Shing Institute of Health Sciences; School of Biomedical Sciences, The Chinese University of Hong Kong

Despite the advancements in multiplex immunohistochemistry and multispectral imaging, characterizing the density and clustering of major immune cells simultaneously in the endometrium remains a challenge. This paper describes a detailed multiplex staining protocol and imaging for the simultaneous localization of four immune cell types in the endometrium.

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