JoVE | Peer Reviewed Scientific Video Journal

Immunology and Infection

Saturated Fatty Acids Induce Ceramide-associated Macrophage Cell Death

Yuwen Zhang¹, Jiaqing Hao¹, Yanwen Sun¹, Bing Li¹

¹Department of Microbiology and Immunology, University of Louisville

We illustrate a straight-forward method to derive murine primary macrophages from bone marrow cells and a simple method to prepare BSA-fatty acid conjugates. Then we demonstrate that saturated fatty acids can induce macrophage cell death, and such cell death is positively associated with cellular accumulation of ceramide levels.

Medicine

A Silicosis Mouse Model Established by Repeated Inhalation of Crystalline Silica Dust

Hangbing Cao^*1,2,3,4, Bing Li^*1,2,3,4, Haoming Chen^1,3,4, Yehong Zhao^1,4, Yuanjie Zou^1,4, Yang Liu^1,4, Min Mu^1,2,3,4, Xinrong Tao^1,2,3,4

¹Key Laboratory of Industrial Dust Control and Occupational Health of the Ministry of Education, Anhui University of Science and Technology, ²Key Laboratory of Industrial Dust Deep Reduction and Occupational Health and Safety of Anhui Higher Education Institutes, Anhui University of Science and Technology, ³Anhui Province Engineering Laboratory of Occupational Health and Safety, ⁴School of Medicine, Department of Medical Frontier Experimental Center, Anhui University of Science and Technology

This protocol describes a method for establishing a mouse model of silicosis through repeated exposure to silica suspensions via a nasal drip. This model can efficiently, conveniently, and flexibly mimic the pathological process of human silicosis with high repeatability and economy.

Medicine

Using Nicotine in a Silica-Exposed Mouse Model to Promote Lung Epithelial-Mesenchymal Transition

Haoming Chen^*1,2,3,4, Bing Li^*1,2,3,4, Hangbing Cao^1,2,3,4, Yehong Zhao^1,2,3,4, Yuanjie Zou^1,2,3,4, Wenyang Wang^1,2,3,4, Min Mu^1,2,3,4, Xinrong Tao^1,2,3,4

¹Key Laboratory of Industrial Dust Control and Occupational Health of the Ministry of Education, Anhui University of Science and Technology, ²Key Laboratory of Industrial Dust Deep Reduction and Occupational Health and Safety of Anhui Higher Education Institutes, Anhui University of Science and Technology, ³Anhui Province Engineering Laboratory of Occupational Health and Safety, ⁴School of Medicine, Department of Medical Frontier Experimental Center, Anhui University of Science and Technology

This study describes a mouse model to study the synergistic effect of nicotine on the progression of pulmonary fibrosis in experimental silicosis mice. The dual-exposure mouse model simulates the pathological progression in the lung after simultaneous exposure to nicotine and silica. The methods described are simple and highly reproducible.

Anhui Province Engineering Laboratory of Occupational Health and Safety

3 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

Medicine

Medicine