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University of Oviedo

4 ARTICLES PUBLISHED IN JoVE

Behavior

Stereoacuity Improvement using Random-Dot Video Games

Santiago Martín-González¹, Juan Portela-Camino², Javier Ruiz-Alcocer³, Igor Illarramendi-Mendicute⁴, Rafaela Garrido-Mercado⁵

¹Department of Construction and Manufacturing Engineering, University of Oviedo, ²Department of Optometry, Clinic Begira, ³Department of Health Sciences, Complutense University of Madrid, ⁴Department of Optometry, Begitek Clinic, ⁵School of Optometry, Complutense University of Madrid

Presented here is a protocol to improve stereoacuity using gamified perceptual learning software based on random-dot stimuli. Patients are stereo-deficient subjects without strabismus. The protocol combines optometry center visits with home exercises using software. Compliance and stereoacuity evolution are stored in the cloud.

JoVE Core

Multimodal Protocol for Assessing Metacognition and Self-Regulation in Adults with Learning Difficulties

Rebeca Cerezo¹, Estrella Fernández¹, Cristina Gómez¹, Miguel Sánchez-Santillán¹, Michelle Taub², Roger Azevedo²

¹University of Oviedo, ²University of Central Florida

The current work proposes a multimodal evaluation protocol focused on metacognitive, self-regulation of learning, and emotional processes, which make up the basis of the difficulties in adults with LDs.

Biology

Immunophenotyping and Cell Sorting of Human MKs from Human Primary Sources or Differentiated In Vitro from Hematopoietic Progenitors

Andrea Acebes-Huerta¹, Patricia Martínez-Botía¹, Cristina Martín Martín², Ángel Bernardo^1,3, Adrián R. Rodríguez¹, María Carmen Vicente-Ayuso⁴, Celina Benavente Cuesta⁵, Laura Gutiérrez^1,6

¹Platelet Research Lab, Instituto de Investigación Sanitaria del Principado de Asturias (ISPA), ²Flow Cytometry and Cell Sorting Platform (ISPA), ³Clinical Diagnosis Laboratory - Dept. of Hematology, Hospital Universitario Central de Asturias (HUCA), ⁴Department of Hematology, Hospital Universitario Severo Ochoa (HUSO), ⁵Department of Hematology, Instituto de Investigación Sanitaria San Carlos (IdISSC), Hospital Clínico San Carlos (HCSC), ⁶Dept. of Medicine, University of Oviedo

Here, we present an immunophenotyping strategy for the characterization of megakaryocyte differentiation, and show how that strategy allows the sorting of megakaryocytes at different stages with a fluorescence-activated cell sorter. The methodology can be applied to human primary tissues, but also to megakaryocytes generated in culture in vitro.

Biology

Versatility of Protocols for Resistance Training and Assessment Using Static and Dynamic Ladders in Animal Models

Eduardo Iglesias-Gutiérrez^1,2, Manuel Fernández-Sanjurjo², Álvaro F. Fernández³, Francisco José Rodríguez Díaz⁴, Isabel López-Taboada^5,6, Cristina Tomás-Zapico^1,2, Benjamín Fernández-García^2,7

¹Department of Functional Biology, University of Oviedo, ²Instituto de Investigación Sanitaria del Principado de Asturias (ISPA), ³Department of Biochemistry and Molecular Biology, University of Oviedo, ⁴Animal facilities, University of Oviedo, ⁵Department of Psychology, University of Oviedo, ⁶Instituto de Neurociencias del Principado de Asturias (INEUROPA), University of Oviedo, ⁷Department of Morphology and Cellular Biology, University of Oviedo

The present protocol describes resistance training and testing using static and dynamic ladders in animal models.