May 19th, 2010
•We describe a small molecule-based protocol for differentiation of mouse embryonic stem cells into oligodendrocyte precursor cells (OPCs). This protocol generates Olig2+NG2+ OPCs with high efficiency by 30 days of differentiation. We also describe a method to generate "spiking" OPCs that can fire action potentials.
Tags
Vídeos Relacionados
Propagation of Human Embryonic Stem (ES) Cells
Culture of Mouse Neural Stem Cell Precursors
Derivation of Hematopoietic Stem Cells from Murine Embryonic Stem Cells
Derivation of Human Embryonic Stem Cells by Immunosurgery
Chromatin Immunoprecipitation from Human Embryonic Stem Cells
In vitro Differentiation of Mouse Embryonic Stem (mES) Cells Using the Hanging Drop Method
Isolation and Derivation of Mouse Embryonic Germinal Cells
Efficient Derivation of Human Cardiac Precursors and Cardiomyocytes from Pluripotent Human Embryonic Stem Cells with Small Molecule Induction
Efficient Differentiation of Mouse Embryonic Stem Cells into Motor Neurons
Differentiation of Newborn Mouse Skin Derived Stem Cells into Germ-like Cells In vitro
SOBRE A JoVE
Copyright © 2024 MyJoVE Corporation. Todos os direitos reservados