June 19th, 2012
•We describe here a technique that is now routinely used to isolate stably bound ribosome nascent chain complexes (RNCs). This technique takes advantage of the discovery that a 17 amino acid long SecM "arrest sequence" can halt translation elongation in a prokaryotic (E. coli) system, when inserted into (or fused to the C-terminus) of virtually any protein.
Tags
Vídeos Relacionados
Microfabricated Post-Array-Detectors (mPADs): an Approach to Isolate Mechanical Forces
Correlative Light and Electron Microscopy (CLEM) as a Tool to Visualize Microinjected Molecules and their Eukaryotic Sub-cellular Targets
Electroantennographic Bioassay as a Screening Tool for Host Plant Volatiles
Isolation of Ribosome Bound Nascent Polypeptides in vitro to Identify Translational Pause Sites Along mRNA
Using Caenorhabditis elegans as a Model System to Study Protein Homeostasis in a Multicellular Organism
Examination of Proteins Bound to Nascent DNA in Mammalian Cells Using BrdU-ChIP-Slot-Western Technique
Laser-assisted Microdissection (LAM) as a Tool for Transcriptional Profiling of Individual Cell Types
Magnetic-Activated Cell Sorting Strategies to Isolate and Purify Synovial Fluid-Derived Mesenchymal Stem Cells from a Rabbit Model
A High Output Method to Isolate Cerebral Pericytes from Mouse
Transposon-insertion Sequencing as a Tool to Elucidate Bacterial Colonization Factors in a Burkholderia gladioli Symbiont of Lagria villosa Beetles
SOBRE A JoVE
Copyright © 2024 MyJoVE Corporation. Todos os direitos reservados