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JoVE Journal

Bioengineering

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Microfluidic Platform for Measuring Neutrophil Chemotaxis from Unprocessed Whole Blood

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10:13 min

June 3rd, 2014

June 3rd, 2014

11,144 Views

0:05

Title

2:22

Microfluidic Device Fabrication

4:19

Microfluidic Assay Preparation

6:11

Sample Preparation: Human Neutrophils From Capillary Blood

6:49

Microscopy and Image Analysis for Neutrophil Chemotaxis Measurements

7:53

Results: Neutrophils Chemotaxis Assay From Whole Blood

8:55

Conclusion

This protocol details an assay designed to measure human neutrophil chemotaxis from one droplet of whole blood with robust reproducibility. This approach circumvents the need for neutrophil separation and requires only a few minutes of assay preparation time. The microfluidic chip enables the repeated measure of neutrophil chemotaxis over time in infants or small mammals, where sample volume is limited.

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