October 25th, 2014
•Here we describe biochemical assays that can be used to characterize ATP-dependent chromatin remodeling enzymes for their abilities to 1) catalyze ATP-dependent nucleosome sliding, 2) engage with nucleosome substrates, and 3) hydrolyze ATP in a nucleosome- or DNA-dependent manner.
Tags
Vídeos Relacionados
A Chromatin Assay for Human Brain Tissue
Zebrafish Brain Ventricle Injection
Analysis of Schwann-astrocyte Interactions Using In Vitro Assays
Photobleaching Assays (FRAP & FLIP) to Measure Chromatin Protein Dynamics in Living Embryonic Stem Cells
Quantitative Comparison of cis-Regulatory Element (CRE) Activities in Transgenic Drosophila melanogaster
Blood Collection for Biochemical Analysis in Adult Zebrafish
Biochemical and High Throughput Microscopic Assessment of Fat Mass in Caenorhabditis Elegans
Biochemical Titration of Glycogen In vitro
Viability Assays for Cells in Culture
Generation and Purification of Human INO80 Chromatin Remodeling Complexes and Subcomplexes
SOBRE A JoVE
Copyright © 2024 MyJoVE Corporation. Todos os direitos reservados