October 23rd, 2014
•This protocol describes a procedure for generating and purifying wild type and mutant versions of the human INO80 chromatin remodeling complex. Epitope tagged versions of INO80 subunits are stably expressed in HEK293 cells, and complete complexes and complexes lacking specific sets of subunits are purified by immunoaffinity chromatography.
Tags
Vídeos Relacionados
Purification and Visualization of Influenza A Viral Ribonucleoprotein Complexes
Human Pancreatic Islet Isolation: Part II: Purification and Culture of Human Islets
Staining of Proteins in Gels with Coomassie G-250 without Organic Solvent and Acetic Acid
Generation of Human CD40-activated B cells
Visualization of the Interstitial Cells of Cajal (ICC) Network in Mice
Chromatin Isolation by RNA Purification (ChIRP)
Generation, Purification, and Characterization of Cell-invasive DISC1 Protein Species
Generation of High Quality Chromatin Immunoprecipitation DNA Template for High-throughput Sequencing (ChIP-seq)
Efficient Production and Purification of Recombinant Murine Kindlin-3 from Insect Cells for Biophysical Studies
Biochemical Assays for Analyzing Activities of ATP-dependent Chromatin Remodeling Enzymes
SOBRE A JoVE
Copyright © 2024 MyJoVE Corporation. Todos os direitos reservados