Edith Suss-Toby is thanked for her assistance in the time-lapse microscopy and image analysis. Nofar Rada is thanked for the artistic work.

Quatro a seis semanas de idade C57BL feminino / camundongos CJ (Harlan, Jerusalém, Israel) foram usados ​​no experimento de acordo com a Associação de Avaliação e Acreditação de especificações Laboratory Animal Care. Todos os procedimentos experimentais foram realizados de acordo com o Animal Care Institucional e Comitê de Uso e do Departamento de Agricultura regulamentos. 1. colheita da Medula Espinhal <ol><li> Euthanize o rato usando uma câmara de CO 2. Evite deslocamento cervical, uma vez que pode causar danos às raízes ganglionares devido a forças de cisalhamento. Daqui em diante, executar todas as etapas sob condições estéreis. </li><li> Mergulhe animal para baixo, com 70% de etanol. Esta etapa é importante para a esterilização e para a prevenção do cabelo de arrastando através dos órgãos internos. </li><li> Deixar o mouse para secar a partir do etanol. Evitar qualquer contacto de etanol com os órgãos internos, devido à neurotoxicidade. </li><li> Após a secagem do rato, posicioná-lo utilizando os pinos na posição prona(Face para baixo). Coloque os pinos de cada membro posterior e membro anterior. </li><li> Usando fórceps, fazer uma incisão na linha média que se estende a partir do gargalo craniocaudal posterior à coluna lombar. Em seguida, levante retalhos cutâneos bilateralmente usando uma pinça e expor o tecido subcutâneo. </li><li> Palpar crânio mouse até chegar a junção crânio-cervical. Com uma tesoura perpendiculares ao animal, cortar os músculos cervicais e coluna na junção craniocervical (7ª vértebras cervicais), usando uma pinça pesados. Dissecar a espinha caudal e extirpar a coluna com uma pinça pesados ​​até atingir o nível lombar (traseira nível membros). </li><li> Executar uma transecção caudal completa ao nível da coluna lombar (5ª vértebras lombares) usando uma pinça pesados. </li><li> Passe o mouse sobre (voltada para cima). Não há necessidade de cobrir a incisão desde o DRG é extraído a partir de níveis vertebrais mais baixos e não o colo do útero. </li><li> Cortadas na linha média do pescoço até o abdômen, retrair opele lateralmente e, em seguida, cortado para abrir o peritoneu. </li><li> Remover os órgãos internos no interior do peritoneu (fígado, baço, pâncreas, estômago, intestino) e em bloco. Em seguida, remover os órgãos retroperitoneais (ou seja, rins e pâncreas). Cranial, abra a parede torácica. </li><li> Usando fórceps e uma lâmina cirúrgica, cortar as nervuras, deixando 5 mm de nervuras bilateralmente longe da coluna vertebral. Neste ponto, a coluna vertebral é separada do resto do corpo. </li><li> Lava-se a coluna vertebral de sangue com frio (4 ° C) fresco salina tamponada com fosfato (PBS) duas vezes. </li></ol> 2. Isolar o Root Ganglia dorsal (DRG) <ol><li> Use um estereomicroscópio com uma ampliação de 4X. </li><li> Posicionar a coluna vertebral em uma plataforma absorvente non non-aderente (Telfa / nylon). Posicione espinha enfrentam-se na mesma orientação crânio-caudal. </li><li> Remova todos os músculos da coluna vertebral e do tecido conjuntivo. Use as costelas como um marco para os nervos como elesdeixar a coluna vertebral. Costelas também protegem os nervos dos instrumentos cirúrgicos. O DRG estão localizados nas regiões cervical, torácica, lombar e locais das vértebras. </li><li> Em seguida, usando uma tesoura cortou o corpo vertebral na linha média e expor a espinal medula e raízes DRG por retracção suave de lateral do corpo vertebral. </li><li> No espaço intercostal, siga o nervo periférico medial ao longo da nervura lateral, para o DRG. </li><li> Identificar o DRG. Parece que a gema de &quot;ovo frito&quot; deitado no nervo. </li><li> Cortar o distai do nervo intercostal para o DRG deixando 2-3 mm de nervo distal à gânglios, a ser utilizado para a retracção. Esta &quot;cauda&quot; será removido após a colheita. </li><li> Segure o nervo usando uma pinça. Comprimindo o próprio corpo DRG irá causar danos às células neurais e deve ser evitada. </li><li> Aproxime-se do DRG proximal ao longo do seu apego à medula espinhal, através da sua anterior e raízes posteriores. </li><li> Aplicar retração suave para a DRG puxando a efnervo dife- (coto do nervo intercostal) lateralmente, e cortar o anterior e raízes posteriores perto do DRG. </li><li> Manter o DRG em uma placa de Petri de 35 milímetros preenchido com DMEM fresco, arrefecido em gelo suplementado com 10% de soro fetal de vitelo (FCS), 1% de penicilina-estreptomicina, aminoácidos não essenciais a 1%, e 1% de piruvato de sódio. </li></ol> 3. Implantação na placa de Petri <ol><li> Execute os próximos passos no gelo utilizando pontas de pipeta pré-resfriado para evitar a ECM solidificação. </li><li> Sob um microscópio estereoscópico no 2-4X ampliação, coloque um copo placa de petri fundo 35 mm numa grade de papel. Importante: Trabalho em gelo, a fim de manter o ECM no estado líquido. Criar um ponto de diâmetro de 1 mm (aproximadamente 20 ul) de crescimento ECM empobrecido-fator no centro da grade. </li><li> Sob visualização directa, colocar o DRG no centro do ponto de ECM perto do fundo do prato. Nota: As células cancerosas utilizados neste protocolo são células de câncer pancreático murino (KPC) establisele tinha a partir de amostras de tumor isoladas de fresco de ratinhos KPC como descrito noutro local 19. </li><li> células de cancro da colheita a partir de culturas confluentes de 40.000, lavá-los uma vez com PBS, e ressuspender-los em 40 ul de ECM em gelo. </li><li> Sob visualização directa da rede usando um microscópio estereoscópico mm medida 500 em cada direção a partir do DRG. Neste ponto lentamente semear células 10.000 / 10 ul ECM. Use uma ponta prï¿½arrefecida 2-10 ul para injetar as células próximas ao fundo do prato, evitando a sua propagação na matriz ou descolamento da matriz. (Figura 1). </li><li> Deixe o prato dentro de uma câmara de fluxo laminar para 10-15 minutos para solidificar. Evitar a secagem ECM. </li><li> Lentamente adicionar DMEM, preparado tal como mencionado anteriormente, contra a parede lateral da placa. Adicionar meio suficiente para cobrir o ECM (cerca de 2 mL). </li><li> Coloque o prato de volta na incubadora a 37 ° C. </li><li> Substituir o meio com meio fresco no dia seguinte. </li><li> Substituir o meio a cada 2 dias. &lt;/ Li&gt; </ol> 4. Aquisição de Dados, Time-lapse Videomicroscopia <ol><li> No dia 7 após a implantação, levar o prato para a microscopia de lapso de tempo. Note-se que certas células pode exigir a aquisição anteriormente devido à migração mais rápida. </li><li> Durante a imagens ao vivo, colocar o prato num ambiente fechado, a uma temperatura média de 37 ± 0,1 ° C e 5% de CO 2. </li><li> Gravar as células utilizando uma câmara de carga acoplado dispositivo colocado sobre o microscópio. </li><li> Aqui imagens digitalizadas a cada 10 min até 72 h, para seguir a locomoção celular de três a seis células diferentes. </li><li> Substituir médio após 24 horas. </li><li> Executar aquisição de dados e análise simples usando o software microscópio. </li></ol> Análise 5. Os dados <ol><li> Para aplicações mais avançadas (ou seja, rastreamento de celular em 2D ou 3D) usar softwares especializados (como Imaris 4D). Nota: Este software determina as coordenadas xy de uma célula emqualquer momento dado, juntamente com a distância desde a origem, e a velocidade de movimento. </li><li> Calcula-se a velocidade média através da medição da distância de deslocamento entre as posições subsequentes em intervalos de 10 min. Calcular o Índice de migração para a frente (IMF), que é a distância da frente da célula a partir da neurite e descreve o movimento unidireccional das células para o DRG. </li></ol>

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This research was supported by the ICRF Barbara S. Goodman endowed research career development award (2011-601-BGPC) and a grant from the US-Israel Binational Science Foundation. No other financial disclosures from the authors are to be made.

Este artigo apresenta um modelo in vitro que recapitula o microambiente canceroso no nicho neural, o modelo DRG. O vídeo demonstra todos os passos a partir de reconhecer marcos anatômicos, tais como o DRG no ratinho, a sua extracção e, finalmente, a sua cultura em ECM. Co-cultura de DRG juntamente com as células cancerosas, também é apresentado. Não há outros modelos para a investigação in vitro de invasão perineural descritos na literatura tornando este modelo essencial para estud...

tumores sólidos divulgação de três maneiras principais: invasão direta, disseminação linfática e disseminação hematogênica. No entanto, há um quarto meio de disseminação do câncer que é frequentemente ignoradas, disseminação ao longo dos nervos. Invasão cancerosa neural (CNI) é um percurso bem conhecido de disseminação do cancro, especialmente em tipos de cancro da cabeça e do pescoço, um de próstata 2, 3 e pâncreas. 4-8 CNI ocorre em mais de 80% dos indivíduos com adenocarcinoma do pâncreas, levando a tumor retroperitonial espalhar através dos nervos gânglio celíaco. Estas células cancerosas neurotrópico têm uma capacidade única para migrar unidireccionalmente ao longo dos nervos em relação ao sistema nervoso central (SNC). 9 Esta descoberta sugere que o microambiente perineural pode ser explorada por células cancerosas, proporcionando factores que suportam o crescimento maligno. Um dos poucos modelos in vitro para a pesquisa CNI é o gânglio da raiz dorsal (DRG) / modelo de células de cancro. este modEL é frequentemente utilizado para estudar a interacção entre as células do estroma parácrina e cancerosas neurais. 10-18 Neste modelo, rato ou linhas celulares de cancro humano são cultivadas em matriz extracelular (ECM) adjacente às preparações de DRG dissociados cultivadas de fresco. Este artigo vídeo mostra a aplicação do in vitro CNI em adenocarcinoma ductal pancreático.

<table><tbody><tr><td>&lt;strong&gt;Equipment:&lt;/strong&gt;</td><td></td><td></td><td></td></tr><tr><td>Operating microscope</td><td>Leica</td><td>M205</td><td></td></tr><tr><td>Time Lapse System</td><td>Zeiss</td><td></td><td></td></tr><tr><td>Forceps</td><td>Sigma-Aldrich</td><td>F4142&amp;nbsp;</td><td></td></tr><tr><td>Surgical blade</td><td>Sigma-Aldrich</td><td>Z309036</td><td></td></tr><tr><td>Scissors</td><td>Sigma-Aldrich</td><td>S3271</td><td></td></tr><tr><td>35 mm Petri dishes, glass bottom</td><td>de groot</td><td>60-627860</td><td></td></tr><tr><td>&lt;strong&gt;Name&lt;/strong&gt;</td><td>&lt;strong&gt;Company&amp;nbsp;&lt;/strong&gt;</td><td>&lt;strong&gt;Catalog Number&lt;/strong&gt;</td><td>&lt;strong&gt;Comments&lt;/strong&gt;</td></tr><tr><td>&lt;strong&gt;Materials:&lt;/strong&gt;</td><td></td><td></td><td></td></tr><tr><td>70% ethanol</td><td>Sigma</td><td></td><td></td></tr><tr><td>Cold PBS</td><td>Biological industries</td><td>02-023-1A</td><td></td></tr><tr><td>DMEM</td><td>Biological industries</td><td>01-055-1A</td><td></td></tr><tr><td>FCS</td><td>Rhenium</td><td>10108165</td><td></td></tr><tr><td>Penicillin and streptomycin</td><td>Biological industries</td><td>01-031-1B</td><td></td></tr><tr><td>Sodium Pyruvate</td><td>Biological industries</td><td>03-042-1B</td><td></td></tr><tr><td>L-Glutamine</td><td>Biological industries</td><td>03-020-1B</td><td></td></tr><tr><td>Growth factor-depleted matrigel</td><td>Trevigen</td><td>3433-005-01</td><td></td></tr></tbody></table>

in vitro modeling of cancerous neural invasion: the dorsal root ganglion model

One way that solid tumors disseminate is through neural invasion. This route is well-known in cancers of the head and neck, prostate, and pancreas. These neurotropic cancer cells have a unique ability to migrate unidirectionally along nerves towards the central nervous system (CNS). The dorsal root ganglia (DRG)/cancer cell model is a three dimensional (3D) in vitro model frequently used for studying the interaction between neural stroma and cancer cells. In this model, mouse or human cancer cell lines are grown in ECM adjacent to preparations of freshly dissociated cultured DRG. In this article, the DRG isolation protocol from mice, and implantation in petri dishes for co-culturing with pancreatic cancer cells are demonstrated. Five days after implantation, the cancer cells made contact with the DRG neurites. Later, these cells formed bridgeheads to facilitate more extensive polarized, neurotropic migration of cancer cells.

<html> Usando imagens de microscopia de vídeo, o DRG pode ser visto brotando neurites 5-7 dias após o implante, enquanto as células cancerosas migrar longe de suas colônias em direção ao DRG. No 7 ° dia após a implantação, as células cancerosas entrem em contacto com as neurites (Figura 2). O índice de migração para a frente das células de cancro do pâncreas utilizados no protocolo é...

Watch this Scientific Journal Video about In Vitro Modeling of Cancerous Neural Invasion: The Dorsal Root Ganglion Model at JoVE.com

In Vitro Modeling of Cancerous Neural Invasion: The Dorsal Root Ganglion Model

This video article shows the use of the dorsal root ganglia (DRG)/cancer cell model in pancreatic ductal adenocarcinoma.

In Vitro Modelagem de Cancerous Neural Invasion: o gânglio Modelo de raiz dorsal

One way that solid tumors disseminate is through neural invasion. This route is well-known in cancers of the head and neck, prostate, and pancreas. These ...

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Neuroscience

In Vitro Modeling of Cancerous Neural Invasion: The Dorsal Root Ganglion Model

9.0K visualizações.  Rambam Medical Healthcare Campus. This video article shows the use of the dorsal root ganglia (DRG)/cancer cell model in pancreatic ductal adenocarcinoma.

Vídeo: In Vitro Modeling of Cancerous Neural Invasion: The Dorsal Root Ganglion Model

A Model for Perineural Invasion in Head and Neck Squamous Cell Carcinoma

Perineural invasion (PNI) is found in approximately 40% of head and neck squamous cell carcinomas (HNSCC). Despite multimodal treatment with surgery, radiation, and chemotherapy, locoregional recurrences and distant metastases occur at higher rates, and overall survival is decreased by 40% compared to HNSCC without PNI. In vitro studies of the pathways involved in HNSCC PNI have historically been challenging given the lack of a consistent, reproducible assay. Described here is the adaptation of the dorsal root ganglion (DRG) assay for the examination of PNI in HNSCC. In this model, DRG are harvested from the spinal column of a sacrificed nude mouse and placed within a semisolid matrix. Over the subsequent days, neurites are generated and grow in a radial pattern from the cell bodies of the DRG. HNSCC cell lines are then placed peripherally around the matrix and invade preferentially along the neurites toward the DRG. This method allows for rapid evaluation of multiple treatment conditions, with very high assay success rates and reproducibility.

Perineural invasion (PNI) is a common feature of head and neck squamous cell carcinoma (HNSCC), conferring lower survival rates. Its mechanisms are poorly understood. Utilizing neurites generated from murine dorsal root ganglia confined to a semisolid matrix, the pathways involved in the PNI of HNSCC cell lines can be investigated.

Um Modelo para Perineural Invasion em cabeça e pescoço carcinoma espinocelular

Perineural invasion (PNI) is found in approximately 40% of head and neck squamous cell carcinomas (HNSCC). Despite multimodal treatment with surgery, ...

Pesquisa do câncer

An In Vivo Murine Sciatic Nerve Model of Perineural Invasion

Cancer cells invade nerves through a process termed perineural invasion (PNI), in which cancer cells proliferate and migrate in the nerve microenvironment. This type of invasion is exhibited by a variety of cancer types, and very frequently is found in pancreatic cancer. The microscopic size of nerve fibers within mouse pancreas renders the study of PNI difficult in orthotopic murine models. Here, we describe a heterotopic in vivo model of PNI, where we inject syngeneic pancreatic cancer cell line Panc02-H7 into the murine sciatic nerve. In this model, sciatic nerves of anesthetized mice are exposed and injected with cancer cells. The cancer cells invade in the nerves proximally toward the spinal cord from the point of injection. The invaded sciatic nerves are then extracted and processed with OCT for frozen sectioning. H&amp;E and immunofluorescence staining of these sections allow quantification of both the degree of invasion and changes in protein expression. This model can be applied to a variety of studies on PNI given its versatility. Using mice with different genetic modifications and/or different types of cancer cells allows for investigation of the cellular and molecular mechanisms of PNI and for different cancer types. Furthermore, the effects of therapeutic agents on nerve invasion can be studied by applying treatment to these mice.

An In Vivo Murine Sciatic Nerve Model of Perineural Invasion

We describe an in vivo murine model of perineural invasion by injecting syngeneic pancreatic cancer cells into the sciatic nerve. The model allows for quantification of the extent of nerve invasion, and supports investigation of the cellular and molecular mechanisms of perineural invasion.

Um modelo murino de nervo ciático na Vivo de invasão Perineural

Cancer cells invade nerves through a process termed perineural invasion (PNI), in which cancer cells proliferate and migrate in the nerve ...

Dorsal Root Ganglion Injection and Dorsal Root Crush Injury as a Model for Sensory Axon Regeneration

Achieving axon regeneration after nervous system injury is a challenging task. As different parts of the central nervous system (CNS) differ from each other anatomically, it is important to identify an appropriate model to use for the study of axon regeneration. By using a suitable model, we can formulate a specific treatment based on the severity of injury, the neuronal cell type of interest, and the desired spinal tract for assessing regeneration. Within the sensory pathway, DRG neurons are responsible for relaying sensory information from the periphery to the CNS. We present here a protocol that uses a DRG injection with a viral vector and a concurrent dorsal root crush injury in the lower cervical spinal cord of an adult rat as a model to study sensory axon regeneration. As demonstrated using a control virus, AAV5-GFP, we show the effectiveness of a direct DRG injection in transducing DRG neurons and tracing sensory axons into the spinal cord. We also show the effectiveness of the dorsal root crush injury in denervating the forepaw as an injury model for evaluating axon regeneration. Despite the requirement for specialized training to perform this invasive surgical procedure, the protocol is flexible, and potential users can modify many parts to accommodate their experimental requirements. Importantly, it can serve as a foundation for those in search of a suitable animal model for their studies. We believe that this article will help new users to learn the procedure in a very efficient and effective manner.

This protocol presents the use of a dorsal root ganglion (DRG) injection with a viral vector and a concurrent dorsal root crush injury in an adult rat as a model to study sensory axon regeneration. This model is suitable for investigating the use of gene therapy to promote sensory axon regeneration.

Dorsal Root Injection gânglio da raiz dorsal e lesão por esmagamento como um modelo para Sensorial Axon Regeneração

Achieving axon regeneration after nervous system injury is a challenging task. As different parts of the central nervous system (CNS) differ from each ...

Neurociência

The Chick Chorioallantoic Membrane In Vivo Model to Assess Perineural Invasion in Head and Neck Cancer

Perineural invasion is a phenotype in which cancer surrounds or invades the nerves. It is associated with poor clinical outcome for head and neck squamous cell carcinoma and other cancers. Mechanistic studies have shown that the molecular crosstalk between nerves and tumor cells occurs prior to physical interaction. There are only a few in vivo models to study perineural invasion, especially to investigate early progression, before physical nerve-tumor interactions occur. The chick chorioallantoic membrane model has been used to study cancer invasion, because the basement membrane of the chorionic epithelium mimics that of human epithelial tissue. Here we repurposed the chick chorioallantoic membrane model to investigate perineural invasion, grafting rat dorsal root ganglia and human head and neck squamous cell carcinoma cells onto the chorionic epithelium. We have demonstrated how this model can be useful to evaluate the ability of cancer cells to invade neural tissue in vivo.&#160;

The Chick Chorioallantoic Membrane In Vivo Model to Assess Perineural Invasion in Head and Neck Cancer

Perineural invasion is an aggressive phenotype for head and neck squamous cell carcinomas and other tumors. The chick chorioallantoic membrane model has been used for studying angiogenesis, cancer invasion, and metastasis. Here we demonstrate how this model can be utilized to assess perineural invasion in vivo.

A membrana Chorioalantoica Chick in vivo modelo para avaliar invasão perineural em câncer de cabeça e pescoço

Perineural invasion is a phenotype in which cancer surrounds or invades the nerves. It is associated with poor clinical outcome for head and neck squamous ...

Medicina

Modelo In Vitro Avançado para Estudo da Hipersensibilidade Neuronal em Culturas DRG

Harvesting, Embedding, and Culturing Dorsal Root Ganglia in Multi-compartment Devices to Study Peripheral Neuronal Features

The most common peripheral neuronal feature of pain is a lowered stimulation threshold or hypersensitivity of terminal nerves from the dorsal root ganglia (DRG). One proposed cause of this hypersensitivity is associated with the interaction between immune cells in the peripheral tissue and neurons. In vitro models have provided foundational knowledge in understanding how these mechanisms result in nociceptor hypersensitivity. However, in vitro models face the challenge of translating efficacy to humans. To address this challenge, a physiologically and anatomically relevant in vitro model has been developed for the culture of intact dorsal root ganglia (DRGs) in three isolated compartments in a 48-well plate. Primary DRGs are harvested from adult Sprague Dawley rats after humane euthanasia. Excess nerve roots are trimmed, and the DRG is cut into appropriate sizes for culture. DRGs are then grown in natural hydrogels, enabling robust growth in all compartments. This multi-compartment system offers anatomically relevant isolation of the DRG cell bodies from neurites, physiologically relevant cell types, and mechanical properties to study the interactions between neural and immune cells. Thus, this culture platform provides a valuable tool for investigating treatment isolation strategies, ultimately leading to an improved screening approach for predicting pain.

Autor em destaque: modelos in vitro e in vivo para aumentar a eficácia do tratamento da dor crônica

This protocol provides a technique to harvest and culture explanted dorsal root ganglion (DRG) from adult Sprague Dawley rats in a multi-compartment (MC) device.

Coleta, incorporação e cultura de gânglios da raiz dorsal em dispositivos multicompartimentais para estudar características neuronais periféricas

The most common peripheral neuronal feature of pain is a lowered stimulation threshold or hypersensitivity of terminal nerves from the dorsal root ganglia ...

Bioengenharia

Establishing a Three-dimensional Co-culture Model of Murine Dorsal Root Ganglia and Cancer Cells: An In Vitro Model to Study Perineural Cancer Cell Invasion

Source: Huyett, P. et al. <a href="https://www.jove.com/video/55043" target="_blank">A Model for Perineural Invasion in Head and Neck Squamous Cell Carcinoma.</a> J. Vis. Exp. (2017)
In this video, we demonstrate the procedure for developing an in vitro perineural cancer cell invasion model by co-culturing mouse dorsal root ganglion and neurotropic cancer cells. The model can be utilized to conduct cancer assays, producing rapid and reproducible results.

Estabelecendo um modelo tridimensional de co-cultura de gânglios da raiz dorsal murina e células cancerígenas: um modelo in vitro para estudar a invasão de células cancerígenas perineurais

Source: Huyett, P. et al. A Model for Perineural Invasion in Head and Neck Squamous Cell Carcinoma. J. Vis. Exp. (2017)
In this video, we demonstrate the ...

JoVE Encyclopedia of Experiments

Câncer de cabeça e pescoço

Técnicas e ensaios

Murine Perineural Invasion Model: Generating an In Vivo Model to Simulate Perineural Invasion in Mouse Sciatic Nerve

Source: Deborde, S. et al. <a href="https://www.jove.com/video/56857" target="_blank">An In Vivo Murine Sciatic Nerve Model of Perineural Invasion.</a> J. Vis. Exp. (2018)
In this video, we develop an in vivo mouse model for perineural invasion by injecting pancreatic cancer cells into the sciatic nerve. This model helps quantify the extent of invasion and study the interactions between cancer cells and nerves.

Modelo de Invasão Perineural Murina: Gerando um Modelo In Vivo para Simular a Invasão Perineural no Nervo Ciático de Camundongo

Source: Deborde, S. et al. An In Vivo Murine Sciatic Nerve Model of Perineural Invasion. J. Vis. Exp. (2018)
In this video, we develop an in vivo mouse ...

Câncer do Sistema Nervoso

Estudos com modelo animal

Modeling Cancerous Neural Invasion In Vitro: A Method to Co-culture Dorsal Root Ganglia and Cancer Cells

Source: Na&#39;ara, S. et al. <a href="https://www.jove.com/video/52990" target="_blank">In Vitro Modeling of Cancerous Neural Invasion: The Dorsal Root Ganglion Model.</a> J. Vis. Exp. (2016)
In this video, we demonstrate an in vitro method for co-culturing DRG and cancer cells to study neurotropism and neural invasion.

Modelagem de invasão neural cancerígena in vitro: um método para co-cultura de gânglios da raiz dorsal e células cancerígenas

Source: Na&#39;ara, S. et al. In Vitro Modeling of Cancerous Neural Invasion: The Dorsal Root Ganglion Model. J. Vis. Exp. (2016)
In this video, we ...

In Vitro Modelagem de Cancerous Neural Invasion: o gânglio Modelo de raiz dorsal

Resumo

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Dorsal Root Injection gânglio da raiz dorsal e lesão por esmagamento como um modelo para Sensorial Axon Regeneração

A membrana Chorioalantoica Chick in vivo modelo para avaliar invasão perineural em câncer de cabeça e pescoço

Coleta, incorporação e cultura de gânglios da raiz dorsal em dispositivos multicompartimentais para estudar características neuronais periféricas

Coleta, incorporação e cultura de gânglios da raiz dorsal em dispositivos multicompartimentais para estudar características neuronais periféricas

Coleta, incorporação e cultura de gânglios da raiz dorsal em dispositivos multicompartimentais para estudar características neuronais periféricas

Estabelecendo um modelo tridimensional de co-cultura de gânglios da raiz dorsal murina e células cancerígenas: um modelo in vitro para estudar a invasão de células cancerígenas perineurais

Modelo de Invasão Perineural Murina: Gerando um Modelo In Vivo para Simular a Invasão Perineural no Nervo Ciático de Camundongo

Modelagem de invasão neural cancerígena in vitro: um método para co-cultura de gânglios da raiz dorsal e células cancerígenas