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JoVE Journal

Neuroscience

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Microglia as a Surrogate Biosensor to Determine Nanoparticle Neurotoxicity

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08:37 min

October 25th, 2016

October 25th, 2016

6,860 Views

0:05

Title

1:12

Culturing Microglial Cells

3:47

Measuring Cytokine Release from Microglial Cells

5:42

Using Cultured Hypothalamic Cells for a Toxicity Assay

6:57

Results: Cytokine Release from Microglial after AgNP Exposure

7:54

Conclusion

Transcrição

The overall goal of this protocol is to describe an in vitro surrogate biosensor for testing microglial response to nanoparticles. We will then determine neurotoxicity following exposure to conditioned media from microglia stimulated with nanopart

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Microglia (immune cells of the brain), are used as a surrogate biosensor to determine how nanoparticles influence neurotoxicity. We describe a series of experiments designed to assay microglial response to nanoparticles and exposure of hypothalamic neurons to supernatant from activated microglia to determine neurotoxicity.

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