JoVE | Institution - University of Minnesota

Institution - University of Minnesota

Articles Published in JoVE

High-throughput Flow-cytometry Measurement of Cellular Mechanotype Based on Rupture and Delivery of DNA Tension Probes into Cells

611 views

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2025

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Bioengineering

Matthew R. Pawlak¹, Wendy R. Gordon¹

¹College of Biological Sciences, University of Minnesota

Non-Viral Engineering of Primary Human T Cells via Homology-Mediated End-Joining Targeted Integration of Large DNA Templates

644 views

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2025

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Biology

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Method Collection

Matthew J. Johnson^1,2,3, Anthony P. DeFeo^1,2,3, Nicholas J. Slipek^1,2,3, Timothy D. Folsom^1,2,3, Tom Henley⁴, Modassir S. Choudhry⁴, Beau R. Webber^1,2,3,5, Branden S. Moriarity^1,2,3,5

¹Department of Pediatrics, University of Minnesota, ²Masonic Cancer Center, University of Minnesota, ³Center for Genome Engineering, University of Minnesota, ⁴Intima Bioscience, Inc., ⁵Stem Cell Institute, University of Minnesota

Strand-Specific Analysis of Proteins at Replicating DNA Strands by Enrichment and Sequencing of Protein-Associated Nascent DNA Method

622 views

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2025

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Biology

Srinivasu Karri¹, Quinn Dickinson¹, Zhiming Li², Chuanhe Yu¹, Zhiguo Zhang³

¹Hormel Institute, University of Minnesota, ²West China School of Public Health and West China Fourth Hospital, State Key Laboratory of Biotherapy, Sichuan University, ³Institute for Cancer Genetics, Department of Pediatrics and Department of Genetics and Development, Columbia University Medical Center

A Clinical Metaproteomics Workflow Implemented within Galaxy Bioinformatics Platform to Analyze Host-Microbiome Interactions Underlying Human Disease

940 views

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2025

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Immunology and Infection

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Method Collection

Katherine Do¹, Subina Mehta¹, Reid Wagner², Timothy J. Griffin¹, Pratik D. Jagtap¹

¹Department of Biochemistry, Molecular Biology and Biophysics, University of Minnesota, ²Minnesota Supercomputing Institute, University of Minnesota

Quantifying Drosophila melanogaster Eye Phenotypes: A Computational Approach Integrating ilastik and Flynotyper

1.3K views

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2024

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Developmental Biology

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Method Collection

Qasim Mujteba*¹, Madeleine R. Chalmers*¹, JiHye Kim¹, Minwoo Baek¹, Nam Chul Kim¹

¹Department of Pharmacy Practice and Pharmaceutical Sciences, College of Pharmacy, University of Minnesota

Methodology for Studying Interactions of Vitamin A Membrane Receptors and Opsin Protein with their Ligands in Generating the Retinylidene Protein

1.3K views

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2024

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Biochemistry

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Method Collection

Rakesh Radhakrishnan¹, Anjelynt Lor¹, Dorothy Li¹, Deepti Mudaliar², Glenn P. Lobo¹

¹Department of Ophthalmology and Visual Neurosciences, University of Minnesota, ²High Throughput Screening Laboratory, Institute for Therapeutics Discovery & Development, University of Minnesota

High-resolution Cell Transplantation in Embryonic and Larval Zebrafish

1.3K views

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2024

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Developmental Biology

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Method Collection

Lindsey S. Qian¹, Rabab Ibrahim^1,2, Adam J. Isabella^1,2

¹Department of Genetics, Cell Biology, and Development, University of Minnesota, ²Graduate Program in Molecular, Cellular, Developmental Biology and Genetics, University of Minnesota

A Semi-Automated Workflow for the Cryopreservation of Coral Sperm to Support Biobanking and Aquaculture

1.1K views

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2024

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Biology

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Method Collection

Jonathan Daly^1,2, Rebecca Hobbs¹, Nikolas Zuchowicz³, Mary Hagedorn^4,5, Justine K. O’Brien^1,2

¹Taronga Institute of Science and Learning, Taronga Conservation Society Australia, ²School of Biological, Earth and Environmental Sciences, University of New South Wales, ³Department of Mechanical Engineering, University of Minnesota, ⁴Hawaii Institute of Marine Biology, University of Hawaii, ⁵Smithsonian National Zoo and Conservation Biology Institute

Sample Preparation for In Situ Cryotomography of Mammalian Cells

3.4K views

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2023

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Biochemistry

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Method Collection

Noah Weber*^1,2, Brennan Hinks*^2,3, Jacob Jensen², Thomas Lidahl^2,3, Luiza Mendonça²

¹College of Liberal Arts, University of Minnesota, ²Department of Biochemistry, Molecular Biology and Biophysics, Medical School, University of Minnesota, ³College of Biological Sciences, University of Minnesota

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