The overall goals of these isolation and differentiation protocols are to obtain adipocyte progenitor cells from perivascular adipose tissues, and to induce their differentiation into mature adipocytes. This method allows the analysis of specific
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Here we report a method for isolation of Adipocyte Progenitor Cell (APC) populations from Perivascular Adipose Tissue (PVAT) using Magnetic-activated Cell Sorting (MCS). This method allows for an increased isolation of APC per gram of adipose tissue when compared to Fluorescence-Activated Cell Sorting (FACS).