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JoVE Journal

Immunology and Infection

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Generation of Large Numbers of Myeloid Progenitors and Dendritic Cell Precursors from Murine Bone Marrow Using a Novel Cell Sorting Strategy

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09:05 min

August 10th, 2018

August 10th, 2018

8,969 Views

0:04

Title

0:40

Collection of Murine Bone Marrow Cells

3:57

Staining Strategy

5:53

Set Gates Based on Control Samples

6:22

Collection of Isolated Populations

7:20

Results: Robust Myeloid Progenitors Using Protocol

8:43

Conclusion

Transcrição

This method can enable researchers to acquire sufficient numbers of cell types to answer key questions in the field of developmental immunology. The main advantage of this technique is that it relies on a few select markers to isolate large number

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Here we provide a method for identifying and isolating large numbers of GM-CSF driven myeloid cells using high speed cell sorting. Five distinct populations (Common myeloid progenitors, granulocyte/macrophage progenitors, monocytes, monocyte-derived macrophages, and monocyte-derived DCs) can be identified based on Ly6C and CD115 expression.

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