July 27th, 2018
•Here we present a new approach to identify plant viruses with double-strand DNA genomes. We use standard methods to extract DNA and RNA from infected leaves and carry out next-generation sequencing. Bioinformatic tools assemble sequences into contigs, identify contigs representing virus genomes and assign genomes to taxonomic groups.
Vídeos Relacionados
Isolation and Genome Analysis of Single Virions using 'Single Virus Genomics'
Affinity Purification of Influenza Virus Ribonucleoprotein Complexes from the Chromatin of Infected Cells
Isolation and Analysis of Brain-sequestered Leukocytes from Plasmodium berghei ANKA-infected Mice
New Tools to Expand Regulatory T Cells from HIV-1-infected Individuals
Rapid Analysis of Chromosome Aberrations in Mouse B Lymphocytes by PNA-FISH
In Vitro Analysis of Myd88-mediated Cellular Immune Response to West Nile Virus Mutant Strain Infection
Neutrophil Isolation and Analysis to Determine their Role in Lymphoma Cell Sensitivity to Therapeutic Agents
Phenotypic and Functional Analysis of Activated Regulatory T Cells Isolated from Chronic Lymphocytic Choriomeningitis Virus-infected Mice
Analysis of Lymphocyte Extravasation Using an In Vitro Model of the Human Blood-brain Barrier
Rescue and Characterization of Recombinant Virus from a New World Zika Virus Infectious Clone
SOBRE A JoVE
Copyright © 2024 MyJoVE Corporation. Todos os direitos reservados