Generation of Induced‐PSCs by Sendai Virus Vector Infection
3:50
Clump‐Passaging
5:15
Results: Generation and Characterization of Induced‐PSCs
6:59
Conclusion
Transcrição
Primate IPSCs are useful but often difficult to obtain due to ethical and technical issues. This protocol gives a most accessible path to the generation and maintenance of primate IPSCs. The use of urinary cells as a primary source of IPSCs has a
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The present protocol describes a method to isolate, expand, and reprogram human and non-human primate urine-derived cells to induced pluripotent stem cells (iPSCs), as well as instructions for feeder-free maintenance of the newly generated iPSCs.