May 29th, 2008
•The advent of site-specific recombinase (SSR) technology and the Cre/lox system has led to numerous advances in molecular biology, and has proven itself as a valuable tool for assessing gene function in transgenic animals. This interview discusses the mechanism of site specific recombination by Cyclization recombinase (Cre) and how the use of this enzyme has led to the development of conditional mutagenesis, which has significant advantages over traditional knock out strategies.
Vídeos Relacionados
Molecular Evolution of the Tre Recombinase
Interview: HIV-1 Proviral DNA Excision Using an Evolved Recombinase
Closed System Cell Culture Protocol Using HYPERStack Vessels with Gas Permeable Material Technology
Principles of Rodent Surgery for the New Surgeon
Discovering Protein Interactions and Characterizing Protein Function Using HaloTag Technology
Reduced-gravity Environment Hardware Demonstrations of a Prototype Miniaturized Flow Cytometer and Companion Microfluidic Mixing Technology
Development of a Quantitative Recombinase Polymerase Amplification Assay with an Internal Positive Control
Inducing a Site Specific Replication Blockage in E. coli Using a Fluorescent Repressor Operator System
Quantification of Site-specific Protein Lysine Acetylation and Succinylation Stoichiometry Using Data-independent Acquisition Mass Spectrometry
Parallel High Throughput Single Molecule Kinetic Assay for Site-Specific DNA Cleavage
SOBRE A JoVE
Copyright © 2024 MyJoVE Corporation. Todos os direitos reservados