36.9K Views
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10:18 min
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February 9th, 2015
DOI :
February 9th, 2015
•0:05
Title
1:30
In Silico Design: Final Plasmid and Overlapping Primers
3:58
Amplification of the DNA Fragments
5:44
DNA Purification Using Magnetic Beads
6:58
Assembly Cloning Reaction and Transformation of the Products in E. coli
8:21
Results: Cloning of CstF-54 and CstF-64 Mutants Under the Transcriptional Regulation of Human Elongation Factor 1α Promoter
9:47
Conclusion
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