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Abstract

Medicine

Simultaneous Electrocardiography Recording and Invasive Blood Pressure Measurement in Rats

Published: January 31st, 2019

DOI:

10.3791/59115

1Division of Cardiology, Department of Internal Medicine, Kaohsiung Medical University Hospital, 2Hematology and Oncology, China Medical University Hospital, 3Department of Internal Medicine, Faculty of Medicine, College of Medicine, Kaohsiung Medical University, 4Center for Lipid Biosciences, Kaohsiung Medical University Hospital, 5Lipid Science and Aging Research Center, Kaohsiung Medical University, 6Institute/Center of Medical Science and Technology, National Sun Yat-sen University

Abstract

For studies related to cardiovascular physiology or pathophysiology, blood pressure (BP) and electrocardiography are basic observational parameters. Research focusing on cardiovascular disease models, potential cardiovascular therapeutic targets or pharmaceutical agents requires assessment of systemic arterial pressure and heart rhythm changes. In situations where radio telemetry systems are not available or affordable, the technique of femoral artery cannulation is an alternative way to obtain intra-arterial pressure waveform recordings and systemic BP measurements. This technique is economical and can be performed with standard equipment in animal facilities. However, invasive arterial pressure recording requires cannulation of small arteries, which can be a challenging surgical skill. Here, we present step-by-step protocols for femoral artery cannulation procedures. Key procedures include the calibration of the data acquisition system, tissue dissection and femoral artery cannulation, and setup of the arterial cannulation system for pressure recording. Surface electrocardiography recording procedures are also included. We also present examples of BP recordings from normotensive and hypertensive rats. This protocol allows reliable direct recordings of systemic BP with simultaneous electrocardiography.

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