Published: September 25th, 2021
Network analysis was applied to evaluate the association of various ecological microbial communities, such as soil, water and rhizosphere. Presented here is a protocol on how to use the WGCNA algorithm to analyze different co-occurrence networks that may occur in the microbial communities due to different ecological environments.
The root microbiome plays an important role in plant growth and environmental adaptation. Network analysis is an important tool for studying communities, which can effectively explore the interaction relationship or co-occurrence model of different microbial species in different environments. The purpose of this manuscript is to provide details on how to use the weighted correlation network algorithm to analyze different co-occurrence networks that may occur in microbial communities due to different ecological environments. All analysis of the experiment is performed in the WGCNA package. WGCNA is an R package for weighted correlation network analysis. The experimental data used to demonstrate these methods were microbial community data from the NCBI (National Center for Biotechnology Information) database for three niches of the rice (Oryza sativa) root system. We used the weighted correlation network algorithm to construct co-abundance networks of microbial community in each of the three niches. Then, differential co-abundance networks among endosphere, rhizoplane and rhizosphere soil were identified. In addition, the core genera in network were obtained by the "WGCNA" package, which plays an important regulated role in network functions. These methods enable researchers to analyze the response of microbial network to environmental disturbance and verify different microbial ecological response theories. The results of these methods show that the significant differential microbial networks identified in the endosphere, rhizoplane and rhizosphere soil of rice.
Microbiome research has important implications for understanding and manipulating ecosystem processes1,2. Microbial populations are interconnected by interacting ecological networks, whose characteristics can affect the response of microorganisms to environmental changes3,4. Furthermore, the properties of these networks affect the stability of microbial communities, and are closely associated with soil function5. Weighted gene correlation network analysis has now been widely applied for research on the relationship between genes....
1. Data Download
2. Optimal power value determination
The representative results in this article were downloaded from the 2014 California Abaker rice root microbiome data in the NCBI database (PRJNA386367)9. The data includes the rhizosphere, rhizoplane, and endosphere microbiome samples from rice plants grown for 14 weeks in a submerged rice field. We used the WGCNA algorithm to select the power value that satisfied the three networks that were close to the scale-free network (Figure 1) and developed three co-expression.......
Correlation networks have been increasingly used in bioinformatics applications. WGCNA is a systems biology method for descriptive analysis of the relationships between various elements of a biological system12. R software package was used in earlier work on WGCNA13,14,15. The package includes functions for network construction, module detection, calculations of topological properties, data simulation, vi.......
The development of this manuscript was supported by funds from National Natural Science Foundation of China-Guizhou Provincial People's Government Karst Science Research Center Project (U1812401), Doctoral Research Project of Guizhou Normal University (GZNUD1), Science and Technology Support Project of Guizhou Province (QKHZCYB459) and the Science and Technology Project of the Guiyang(2-8).
The authors would like to thank Edwards J.A et al for providing rice microbiome data in public databases and support from TopEdit (www.topeditsci.com) for its linguistic assistance during the preparation of this manuscript.....
|The University of Auckland
|R is a free software environment for statistical computing and graphics. It compiles and runs on a wide variety of UNIX platforms, Windows and MacOS.
|The RStudio IDE is a set of integrated tools designed to help you be more productive with R and Python.
|Cytoscape is an open source software platform for visualizing complex networks and integrating these with any type of attribute data.
|The National Center for Biotechnology Information advances science and health by providing access to biomedical and genomic information.
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