Abstract
Medicine
Use of 3D Robotic Ultrasound for In Vivo Analysis of Mouse Kidneys
* These authors contributed equally
ERRATUM NOTICE
Important: There has been an erratum issued for this article. Read more …Abstract
Common modalities for in vivo imaging of rodents include positron emission tomography (PET), computed tomography (CT), magnetic resonance imaging (MRI), and ultrasound (US). Each method has limitations and advantages, including availability, ease of use, cost, size, and the use of ionizing radiation or magnetic fields. This protocol describes the use of 3D robotic US for in vivo imaging of rodent kidneys and heart, subsequent data analysis, and possible research applications. Practical applications of robotic US are the quantification of total kidney volume (TKV), as well as the measurement of cysts, tumors, and vasculature. Although the resolution is not as high as other modalities, robotic US allows for more practical high throughput data collection. Furthermore, using US M-mode imaging, cardiac function may be quantified. Since the kidneys receive 20%-25% of the cardiac output, assessing cardiac function is critical to the understanding of kidney physiology and pathophysiology.
Erratum
Erratum: Use of 3D Robotic Ultrasound for In Vivo Analysis of Mouse KidneysAn erratum was issued for: Use of 3D Robotic Ultrasound for In Vivo Analysis of Mouse Kidneys. An author name was updated, along with the Protocol and Representative Results.
The name of an author was updated from:
Ryan C. Gesser
to:
Ryan C. Gessner
Step 3.1 of the Protocol was updated from:
Turn on the US machine and the heat lamp above the US platforms. Turn on the appropriate anesthesia valves for the corresponding US bays that will be used.
to:
Turn on the US machine and the switch for the internal heating element for the US bays. Turn on the appropriate anesthesia valves for the corresponding US bays that will be used.
Figue 1 in the Representative Results was updated from:
Figure 1: Ultrasound system and mouse placement. (A) Diagram of ultrasound system and location of transducers. (B) View of mice in supine position on ultrasound platform with heat lamp. (C) Example of region of interest (ROI)s in place for area of interest (kidneys) with animal IDs. Please click here to view a larger version of this figure.
to:
Figure 1: Ultrasound system and mouse placement. (A) Diagram of ultrasound system and location of transducers. (B) View of mice in supine position on ultrasound platform. (C) Example of region of interest (ROI)s in place for area of interest (kidneys) with animal IDs. Please click here to view a larger version of this figure.
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