A subscription to JoVE is required to view this content. Sign in or start your free trial.
* These authors contributed equally
To study flight behaviors in a fearful context, we introduce a modified fear conditioning protocol. This protocol ensures that mice consistently exhibit flight behaviors during cue presentation in the fear conditioning.
The appropriate manifestation of defensive behavior in a threatening situation is critical for survival. The prevailing theory suggests that an active defensive behavior, such as jumping or rapid darting, is expressed under high threat imminence or actual threat, whereas passive defensive behavior, such as freezing, is expressed when the threat is predicted, but the threat imminence is relatively low. In classical fear conditioning, subjects typically exhibit freezing as a conditioned defensive response, with little expression of active defensive behavior in most cases. Here, we introduce a modified fear conditioning procedure for mice to observe the transition from freezing to flight and vice versa, involving five repetitive pairings of conditioned stimuli (CS; continuous tone, 8 kHz, 95 dB SPL (sound pressure levels)) and unconditioned stimuli (US; foot shock, 0.9 mA, 1.0 s) over two days. This modified fear conditioning procedure requires a relatively large number of conditioning sessions and conditioning days but does not necessitate a high-intensity foot shock for modest expression of flight behavior. Using the same context for conditioning and salient CS presentations is essential to elicit flight behaviors. This modified fear conditioning procedure is a reliable method for observing active defensive behaviors in mice, providing an opportunity to elucidate the fine mechanisms and characteristics of such behaviors in a fearful context.
The appropriate selection of defensive behaviors under threatening circumstances is crucial for the survival of all animals. Defensive behaviors gradually shift from one to another based on threat proximity, such as the transition between freezing and flight behaviors1,2,3. Dysregulation of these behaviors is often observed in various mental disorders4. Post-traumatic stress disorder (PTSD) is one such disorder characterized by exaggerated defensive behaviors, like panic responses to non-threatening stimuli4.
Classical fear conditioning in rodents is commonly used as a model for PTSD5,6,7, but rodents do not express flight (panic-like) behaviors in this model8. Consequently, the classical fear conditioning model, often referred to as the 'rodent PTSD model,' lacks face validity for PTSD in humans, particularly in capturing flight or panic-like symptoms, which have not been well-studied.
Recently, several modified fear conditioning protocols have successfully demonstrated that rodent subjects exhibit flight behavior during these procedures. For instance, repetitive associations of a conditioned stimulus (CS) and an unconditioned stimulus (US) seven times in a day allowed female rats to exhibit darting behaviors similar to flight behaviors9. In two-day fear conditionings using serial compound stimuli (SCS; composed of tone followed by noise), mice began showing flight behaviors during the noise part of SCS presentations10,11,12. The detailed description of the SCS method is provided in a protocol report13. A three-day fear conditioning with SCS also worked for rats to induce flight behaviors14. However, these new protocols have some limitations. For example, the use of serial cue presentation does not allow for the exclusion of the influence of proximity estimation on defensive behavior. In the case of seven times association of CS-US in rats, the majority of flight responses were observed in females rather than males.
In light of these considerations, we introduce a modified fear conditioning protocol for mice to investigate flight behaviors in a fearful context. Male mice consistently exhibit flight behavior during our modified fear conditioning. In this protocol, the salient tone is used as the CS instead of SCS. Additionally, a minimum of five pairings of CS-US in a day for at least two days, along with fear potentiation by the conditioned context, is required. The protocol provides another option for investigating flight behaviors, complementing previous protocols, depending on the research purpose.
This protocol was conducted in accordance with the guiding principles of the Physiological Society of Japan and received approval from the Animal Care Committee of Kanazawa Medical University (2021-32). All procedures were conducted in compliance with the ARRIVE guidelines. Adult male C57BL/6J mice (3-6 months old) were utilized for the study, and it was previously confirmed that these mice exhibited the flight behaviors described in this manuscript15.
1. Animal preparation
2. Setting up the tools/equipment
3. Behavioral experiment
4. Analysis of defensive behaviors
NOTE: Motion, percentage of freezing, and the number of jumps during CS presentations are analyzed. Details are described below. If possible, analyzing in a double-blind manner would be better.
5. Statistical analysis
Results obtained with the modified fear conditioning in male mice (C57BL/6J; 3-6 months old) are presented, following the schedule shown in Figure 1C. The experiment was designed to investigate how the conditioned context influences the expression of flight behaviors. Two groups were assigned: Group 1 (n = 10) and Group 2 (n = 10). A CS (95 dB SPL) and a US (0.9 mA) were used in this experiment.
On day 1, all mice underwent exposure to 5 conditioned stimulus (CS) ...
The modified fear conditioning protocol introduced in this article is a stable method for investigating flight behaviors in a fearful context. By employing this protocol, we have found that the flight behaviors of mice in the fearful context are triggered by salient stimuli and depend on the context. The characteristics of flight behavior were not well-investigated, as there was no suitable protocol to observe flight behaviors. This protocol will be one of the suitable methods for studying active defensive behaviors in a...
The authors declare no competing interests.
This work was supported partly by KAKENHI Grants JP22K15795 (to T.F.), JP22K09734 (to N.K.), JP21K07489 (to R.Y.), Kanazawa Medical University (C2022-3, D2021-4, to R.Y.) and The Naito Foundation (to T.F.).
Name | Company | Catalog Number | Comments |
Audio speaker | Fostex | FT17H | |
Amplifier | Sony | TA-F500 | |
CMOS camera | Sanwa Supply Inc. | CMS-V43BK | |
Fear conditioning chamber | Panlab S.L.U. | LE116 | |
Food pellets | Nosan | Labo MR standard | |
LED | Yamazen | LT-B05N | |
Microphone | ACO | type 4156N | |
Scramble shocker | Panlab S.L.U. | LE 100-26 | |
Sound card | Behringer | UMC202 | |
Sound software | Syntrillium Software | Cool Edit 2000 | |
Transducer | Panlab S.L.U. | LE 111 |
Request permission to reuse the text or figures of this JoVE article
Request PermissionExplore More Articles
This article has been published
Video Coming Soon
Copyright © 2025 MyJoVE Corporation. All rights reserved