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The protocol shows building a simple and cost-effective rat weight-bearing training model for steroid-induced osteonecrosis of the femoral head using elastic therapeutic tape.
Unlike humans, rats are animals that walk on all fours, while humans are bipedal animals that stand, and the hips are subjected to tremendous pressure when walking and standing. In rat steroid-induced femoral head necrosis models, the biomechanical characteristics of the human hip under higher pressure often need to be simulated. Some scholars try to emulate the state of human hip pressure by making rats bear a certain weight, but fixing the weight-bearing object on the rat is tough. Rats can easily break free of immobilization, and sticking the weight on the rats with adhesive tape will cause the rats to suffocate or die from intestinal obstruction. Our research group used elastic therapeutic tape to perform tension-free immobilization of weight-bearing objects in rats so that the rats could breathe freely and not break away from the immobilization under weight-bearing conditions. Compared to the usual steroid-induced femoral head necrosis rat model, we found that this weight-bearing intervention can aggravate the progression of femoral head necrosis in rats.
The administration of glucocorticoids is the most common risk factor for non-traumatic osteonecrosis of the femoral head (ONFH)1. Numerous pieces of evidence suggest that, in addition to glucocorticoids, the pressure load on the hip joint in patients is also associated with the occurrence of ONFH. Factors such as body weight and physical labor intensity are recognized as risk factors for ONFH2. Multiple clinical studies have demonstrated a close relationship between hip joint loading conditions and the timing and incidence of joint replacement3,4,
The protocol adheres to the ethical guidelines set by the Institutional Animal Care and Use Committee (IACUC) at Beijing University of Chinese Medicine, Protocol number BUCM-4-2022062001-2109. The protocol uses Sprague Dawley (SD) rats (SCXK(Jing)2019-0008), aged 8-10 weeks and weighing 200 g-250 g.
1. Adaptation training
Histopathology analysis
Hematoxylin and eosin staining revealed that in the Control group and Control+Load group, bone trabeculae were intact and arranged regularly. Blood vessel endothelial cells were present in bone dimples, and the cell morphology appeared plump. In contrast, the Model group and Model+Load group exhibited fractured and disordered bone trabeculae, along with a significantly higher number of empty lacunae. The Model+Load group had more empty lacunae compared to the Model group. In.......
Currently, various animals, such as rabbits25, rats26, mice27, pigs28, broilers29, ostriches8, and emus30 can be used to establish models of femoral head necrosis. Among them, rats, mice, and rabbits are the most commonly used species. The rat, as a model for femoral head necrosis, offers numerous advantages. Rats are easy to feed and breed, grow rapidly, .......
This research is an independent study and did not receive any funding.
....Name | Company | Catalog Number | Comments |
15ml centrifuge tube | Corning,USA | 430791 | |
5mm stainless steel bead | Gelisen,China | 5mm | |
Acetic acid | Merck KGaA, Germany | 64-19-7 | |
Anhydrous alcohol | Merck KGaA, Germany | 64-17-5 | |
clay | Mincai stationery,China | 102 | |
Coverslip | Servicebio,China | WMWD-1818 | |
Flat pressure bottle 10ml | BEHNCKE,China | MD10ml | |
Formic acid | Macklin Biochemical ,China | 64-18-6 | |
HE staining kit | Solarbio,China | G1120 | |
HistoCore AUTOCUT | Leica, Germany | 149AUTO00C1 | |
Kinesio tape (elastic therapeutic tape) | Fuluo medicine,China | CL1819 | |
Lipopolysaccharide | Solarbio,China | L8880 | |
Lipopolysaccharides (LPS) | Selleck,USA | S7850Â | |
Manual carbon dioxide euthanasia box | Yuyan,China | LC-500-S1 | |
Methylprednisolone sodium succinate,MPS | AbMole,China | M25573 | |
MicroCT | Â Hiscan,China | Â Hiscan VM Pro | |
Neutral resin | Beijing Zhongshan Golden Bridge Biotechnology l ,China | ZLI-9555 | |
Paraffin | Servicebio,China | WGHB-319213129 | |
Paraformaldehyde | Servicebio,China | G1101-500ML | |
Potassium chloride | Macklin Biochemical ,China |  7447-40-7 | |
Slide | Servicebio,China | WG6012 | |
Treadmill for Rats and mice | Litc Life Science,USA | 801 | |
Xylene | Macklin Biochemical ,China |  1330-20-7 |
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