Petra Schwille

Department of Cellular and Molecular Biophysics, Max-Planck-Institut für Biochemie

Affiliated withMax-Planck-Institut für Biochemie Max Planck Institute of Biochemistry

Research Area

BiochemistryBioengineering

Biography

JoVE Journal Publications

Article Total : 4	Year
Publication title	2018
Publication title	2021
Publication title	2022
Publication title	2025

Other Publications

Article	Year
Fluorescence correlation spectroscopy and fluorescence cross-correlation spectroscopy reveal the cytoplasmic origination of loaded nuclear RISC in vivo in human cells. Nucleic acids research\| PubMed ID: 18842624	2008
Importin 8 is a gene silencing factor that targets argonaute proteins to distinct mRNAs. Cell\| PubMed ID: 19167051	2009
Minimal systems to study membrane-cytoskeleton interactions. Current opinion in biotechnology\| PubMed ID: 22503237	2012
Towards a bottom-up reconstitution of bacterial cell division. Trends in cell biology\| PubMed ID: 23067680	2012
Reconstitution of pole-to-pole oscillations of min proteins in microengineered polydimethylsiloxane compartments. Angewandte Chemie (International ed. in English)\| PubMed ID: 23184489	2013
Myosin motors fragment and compact membrane-bound actin filaments. eLife\| PubMed ID: 23326639	2013
Photoconversion of bodipy-labeled lipid analogues. Chembiochem : a European journal of chemical biology\| PubMed ID: 23512865	2013
Lateral membrane diffusion modulated by a minimal actin cortex. Biophysical journal\| PubMed ID: 23561523	2013
The design of MACs (minimal actin cortices). Cytoskeleton (Hoboken, N.J.)\| PubMed ID: 24039068	2013
Propagation of MinCDE waves on free-standing membranes. Environmental microbiology\| PubMed ID: 24118679	2013
MinC, MinD, and MinE drive counter-oscillation of early-cell-division proteins prior to Escherichia coli septum formation. mBio\| PubMed ID: 24327341	2013
Bacterial cell division: a swirling ring to rule them all? Current biology : CB\| PubMed ID: 24556438	2014
MinCDE exploits the dynamic nature of FtsZ filaments for its spatial regulation. Proceedings of the National Academy of Sciences of the United States of America\| PubMed ID: 24707052	2014
Asymmetric supported lipid bilayer formation via methyl-β-cyclodextrin mediated lipid exchange: influence of asymmetry on lipid dynamics and phase behavior. Langmuir : the ACS journal of surfaces and colloids\| PubMed ID: 24885372	2014
ESCRT-III mediated cell division in Sulfolobus acidocaldarius - a reconstitution perspective. Frontiers in microbiology\| PubMed ID: 24926288	2014
Surface topology assisted alignment of Min protein waves. FEBS letters\| PubMed ID: 24937143	2014
Fluorescence fluctuation microscopy: a diversified arsenal of methods to investigate molecular dynamics inside cells. Current opinion in structural biology\| PubMed ID: 25126766	2014
Reconstitution of self-organizing protein gradients as spatial cues in cell-free systems. eLife\| PubMed ID: 25271375	2014
Toward Spatially Regulated Division of Protocells: Insights into the E. coli Min System from in Vitro Studies. Life (Basel, Switzerland)\| PubMed ID: 25513760	2014
Cytoskeletal pinning controls phase separation in multicomponent lipid membranes. Biophysical journal\| PubMed ID: 25762322	2015
Amphipathic DNA origami nanoparticles to scaffold and deform lipid membrane vesicles. Angewandte Chemie (International ed. in English)\| PubMed ID: 25882792	2015
Adaptive lipid packing and bioactivity in membrane domains. PloS one\| PubMed ID: 25905447	2015
FtsZ Polymers Tethered to the Membrane by ZipA Are Susceptible to Spatial Regulation by Min Waves. Biophysical journal\| PubMed ID: 25954894	2015
Jump-starting life? Fundamental aspects of synthetic biology. The Journal of cell biology\| PubMed ID: 26323686	2015
Introducing a fluorescence-based standard to quantify protein partitioning into membranes. Biochimica et biophysica acta\| PubMed ID: 26342678	2015
Diffusion coefficients and dissociation constants of enhanced green fluorescent protein binding to free standing membranes. Data in brief\| PubMed ID: 26587560	2015
Pattern formation on membranes and its role in bacterial cell division. Current opinion in cell biology\| PubMed ID: 26915065	2016
DNA Nanostructures on Membranes as Tools for Synthetic Biology. Biophysical journal\| PubMed ID: 27119630	2016
Single Particle Plasmon Sensors as Label-Free Technique To Monitor MinDE Protein Wave Propagation on Membranes. Nano letters\| PubMed ID: 27172130	2016
Protein Patterns and Oscillations on Lipid Monolayers and in Microdroplets. Angewandte Chemie (International ed. in English)\| PubMed ID: 27465495	2016
Coordinated recruitment of Spir actin nucleators and myosin V motors to Rab11 vesicle membranes. eLife\| PubMed ID: 27623148	2016
Optical Control of Lipid Rafts with Photoswitchable Ceramides. Journal of the American Chemical Society\| PubMed ID: 27626130	2016
In vitro Reconstitution of a Membrane Switch Mechanism for the Polarity Protein LGL. Journal of molecular biology\| PubMed ID: 27720986	2016
Transport efficiency of membrane-anchored kinesin-1 motors depends on motor density and diffusivity. Proceedings of the National Academy of Sciences of the United States of America\| PubMed ID: 27803325	2016
Diffusion of Single-Pass Transmembrane Receptors: From the Plasma Membrane into Giant Liposomes. The Journal of membrane biology\| PubMed ID: 27826635	2017
Control of lipid domain organization by a biomimetic contractile actomyosin cortex. eLife\| PubMed ID: 28463108	2017
Reconstitution of Protein Dynamics Involved in Bacterial Cell Division. Sub-cellular biochemistry\| PubMed ID: 28500535	2017
Large-scale modulation of reconstituted Min protein patterns and gradients by defined mutations in MinE's membrane targeting sequence. PloS one\| PubMed ID: 28622374	2017
Revolving around constriction by ESCRT-III. Nature cell biology\| PubMed ID: 28659638	2017
Cell-free protein synthesis in micro compartments: building a minimal cell from biobricks. New biotechnology\| PubMed ID: 28690157	2017
Biology and the art of abstraction. Biophysical reviews\| PubMed ID: 28756511	2017
High-Speed Atomic Force Microscopy Reveals the Inner Workings of the MinDE Protein Oscillator. Nano letters\| PubMed ID: 29210266	2018
Optical Control of a Biological Reaction-Diffusion System. Angewandte Chemie (International ed. in English)\| PubMed ID: 29266672	2018
Membrane sculpting by curved DNA origami scaffolds. Nature communications\| PubMed ID: 29476101	2018
There and back again: from the origin of life to single molecules. European biophysics journal : EBJ\| PubMed ID: 29569181	2018
FCS Analysis of Protein Mobility on Lipid Monolayers. Biophysical journal\| PubMed ID: 29605081	2018
Reverse and forward engineering of protein pattern formation. Philosophical transactions of the Royal Society of London. Series B, Biological sciences\| PubMed ID: 29632258	2018
Quantifying Reversible Surface Binding via Surface-Integrated Fluorescence Correlation Spectroscopy. Nano letters\| PubMed ID: 29658275	2018
MinE conformational switching confers robustness on self-organized Min protein patterns. Proceedings of the National Academy of Sciences of the United States of America\| PubMed ID: 29666276	2018
Photophysical Behavior of mNeonGreen, an Evolutionarily Distant Green Fluorescent Protein. Biophysical journal\| PubMed ID: 29706225	2018
MaxSynBio - Avenues towards creating cells from the bottom up. Angewandte Chemie (International ed. in English)\| PubMed ID: 29749673	2018
Liposomes and polymersomes: a comparative review towards cell mimicking. Chemical Society reviews\| PubMed ID: 30177983	2018
Control of Membrane Binding and Diffusion of Cholesteryl-Modified DNA Origami Nanostructures by DNA Spacers. Langmuir : the ACS journal of surfaces and colloids\| PubMed ID: 30253101	2018
The MinDE system is a generic spatial cue for membrane protein distribution in vitro. Nature communications\| PubMed ID: 30258191	2018
Beating Vesicles: Encapsulated Protein Oscillations Cause Dynamic Membrane Deformations. Angewandte Chemie (International ed. in English)\| PubMed ID: 30270475	2018
Light-Induced Printing of Protein Structures on Membranes in Vitro. Nano letters\| PubMed ID: 30295028	2018
Membrane association and remodeling by intraflagellar transport protein IFT172. Nature communications\| PubMed ID: 30409972	2018
In vitro reconstitution of the bacterial cytoskeleton: expected and unexpected new insights. Microbial biotechnology\| PubMed ID: 30411506	2019
Plasmonic Nanosensors Reveal a Height Dependence of MinDE Protein Oscillations on Membrane Features. Journal of the American Chemical Society\| PubMed ID: 30481454	2018
Photo-Induced Depletion of Binding Sites in DNA-PAINT Microscopy. Molecules (Basel, Switzerland)\| PubMed ID: 30513691	2018
Myosin-II activity generates a dynamic steady state with continuous actin turnover in a minimal actin cortex. Journal of cell science\| PubMed ID: 30538127	2018
Single Particle Tracking and Super-Resolution Imaging of Membrane-Assisted Stop-and-Go Diffusion and Lattice Assembly of DNA Origami. ACS nano\| PubMed ID: 30588792	2019
More from less - bottom-up reconstitution of cell biology. Journal of cell science\| PubMed ID: 30718262	2019
Optical manipulation of sphingolipid biosynthesis using photoswitchable ceramides. eLife\| PubMed ID: 30720434	2019
124-Color Super-resolution Imaging by Engineering DNA-PAINT Blinking Kinetics. Nano letters\| PubMed ID: 30864449	2019
Flat-top TIRF illumination boosts DNA-PAINT imaging and quantification. Nature communications\| PubMed ID: 30894522	2019
Temperature-sensitive protein expression in protocells. Chemical communications (Cambridge, England)\| PubMed ID: 31094370	2019
Manfred Eigen (1927-2019). Angewandte Chemie (International ed. in English)\| PubMed ID: 31124258	2019
Synthetic cell division via membrane-transforming molecular assemblies. BMC biology\| PubMed ID: 31126285	2019
Design of Sealable Custom-Shaped Cell Mimicries Based on Self-Assembled Monolayers on CYTOP Polymer. ACS applied materials & interfaces\| PubMed ID: 31136146	2019
Bottom-up synthetic biology: reconstitution in space and time. Current opinion in biotechnology\| PubMed ID: 31176995	2019
Fluorescence Correlation Spectroscopy to Examine Protein-Lipid Interactions in Membranes. Methods in molecular biology (Clifton, N.J.)\| PubMed ID: 31218628	2019
The E. coli MinCDE system in the regulation of protein patterns and gradients. Cellular and molecular life sciences : CMLS\| PubMed ID: 31317204	2019
Reconstitution and Coupling of DNA Replication and Segregation in a Biomimetic System. Chembiochem : a European journal of chemical biology\| PubMed ID: 31344304	2019
Heated gas bubbles enrich, crystallize, dry, phosphorylate and encapsulate prebiotic molecules. Nature chemistry\| PubMed ID: 31358919	2019
Toward Absolute Molecular Numbers in DNA-PAINT. Nano letters\| PubMed ID: 31535868	2019
An order of magnitude faster DNA-PAINT imaging by optimized sequence design and buffer conditions. Nature methods\| PubMed ID: 31591576	2019
Design of biochemical pattern forming systems from minimal motifs. eLife\| PubMed ID: 31767054	2019
Shaping Giant Membrane Vesicles in 3D-Printed Protein Hydrogel Cages. Small (Weinheim an der Bergstrasse, Germany)\| PubMed ID: 32105403	2020
Local Self-Enhancement of MinD Membrane Binding in Min Protein Pattern Formation. Journal of molecular biology\| PubMed ID: 32199984	2020
Reversible membrane deformations by straight DNA origami filaments. Soft matter\| PubMed ID: 32406895	2021
Symmetry Breaking and Emergence of Directional Flows in Minimal Actomyosin Cortices. Cells\| PubMed ID: 32527013	2020
The speed of FtsZ treadmilling is tightly regulated by membrane binding. Scientific reports\| PubMed ID: 32591587	2020
Cytoskeletal and Actin-Based Polymerization Motors and Their Role in Minimal Cell Design. Advanced biosystems\| PubMed ID: 32648711	2019
Cell-Free Protein Synthesis and Its Perspectives for Assembling Cells from the Bottom-Up. Advanced biosystems\| PubMed ID: 32648712	2019
Phosphoinositides regulate force-independent interactions between talin, vinculin, and actin. eLife\| PubMed ID: 32657269	2020
FtsZ Reorganization Facilitates Deformation of Giant Vesicles in Microfluidic Traps*. Angewandte Chemie (International ed. in English)\| PubMed ID: 32735732	2020
Fine-Tuning Protein Self-Organization by Orthogonal Chemo-Optogenetic Tools. Angewandte Chemie (International ed. in English)\| PubMed ID: 33155720	2021
Non-Equilibrium Large-Scale Membrane Transformations Driven by MinDE Biochemical Reaction Cycles. Angewandte Chemie (International ed. in English)\| PubMed ID: 33285025	2021
Probing Biomolecular Interactions by a Pattern-Forming Peptide-Conjugate Sensor. Bioconjugate chemistry\| PubMed ID: 33314917	2021
Division in synthetic cells. Emerging topics in life sciences\| PubMed ID: 33523162	2019
Protein Reconstitution Inside Giant Unilamellar Vesicles. Annual review of biophysics\| PubMed ID: 33667121	2021
De novo design of a reversible phosphorylation-dependent switch for membrane targeting. Nature communications\| PubMed ID: 33674566	2021
Reconstitution of contractile actomyosin rings in vesicles. Nature communications\| PubMed ID: 33859190	2021
Increasing MinD's Membrane Affinity Yields Standing Wave Oscillations and Functional Gradients on Flat Membranes. ACS synthetic biology\| PubMed ID: 33881306	2021
FtsZ induces membrane deformations via torsional stress upon GTP hydrolysis. Nature communications\| PubMed ID: 34083531	2021
Membrane-coated 3D architectures for bottom-up synthetic biology. Soft matter\| PubMed ID: 34106121	2021
Molecular-scale visualization of sarcomere contraction within native cardiomyocytes. Nature communications\| PubMed ID: 34215727	2021
Tracking single particles for hours via continuous DNA-mediated fluorophore exchange. Nature communications\| PubMed ID: 34290254	2021
Hydration Layer of Only a Few Molecules Controls Lipid Mobility in Biomimetic Membranes. Journal of the American Chemical Society\| PubMed ID: 34342967	2021
Self-organized protein patterns: The MinCDE and ParABS systems. Current opinion in cell biology\| PubMed ID: 34399108	2021
Actin crosslinker competition and sorting drive emergent GUV size-dependent actin network architecture. Communications biology\| PubMed ID: 34584211	2021
Mass-sensitive particle tracking to elucidate the membrane-associated MinDE reaction cycle. Nature methods\| PubMed ID: 34608318	2021
Hidden protein functions and what they may teach us. Trends in cell biology\| PubMed ID: 34654605	2021
Design Features to Accelerate the Higher-Order Assembly of DNA Origami on Membranes. The journal of physical chemistry. B\| PubMed ID: 34818013	2021
CTP-controlled liquid-liquid phase separation of ParB. Journal of molecular biology\| PubMed ID: 34902429	2022
PyCorrFit-generic data evaluation for fluorescence correlation spectroscopy. Bioinformatics (Oxford, England)\| PubMed ID: 24825612	2014
Dynamics and interaction of interleukin-4 receptor subunits in living cells. Biophysical journal\| PubMed ID: 25468331	2014
Membrane targeting of the Spir·formin actin nucleator complex requires a sequential handshake of polar interactions. The Journal of biological chemistry\| PubMed ID: 25564607	2015
Active shape oscillations of giant vesicles with cyclic closure and opening of membrane necks. Soft matter\| PubMed ID: 32914814	2021
Author Correction: Reconstitution of contractile actomyosin rings in vesicles. Nature communications\| PubMed ID: 35273165	2022
Tracing back variations in archaeal ESCRT-based cell division to protein domain architectures. PloS one\| PubMed ID: 35358274	2022
Dendrimersome Synthetic Cells Harbor Cell Division Machinery of Bacteria. Advanced materials (Deerfield Beach, Fla.)\| PubMed ID: 35579491	2022
3D printed protein-based robotic structures actuated by molecular motor assemblies. Nature materials\| PubMed ID: 35618822	2022
Halogenation of tyrosine perturbs large-scale protein self-organization. Nature communications\| PubMed ID: 35977922	2022
Asgard ESCRT-III and VPS4 reveal conserved chromatin binding properties of the ESCRT machinery. The ISME journal\| PubMed ID: 36221007	2023
In vitro assembly, positioning and contraction of a division ring in minimal cells. Nature communications\| PubMed ID: 36243816	2022
Membrane mediated phase separation of the bacterial nucleoid occlusion protein Noc. Scientific reports\| PubMed ID: 36289351	2022
Prebiotic Foam Environments to Oligomerize and Accumulate RNA. Chembiochem : a European journal of chemical biology\| PubMed ID: 36354762	2022
Calibration-free counting of low molecular copy numbers in single DNA-PAINT localization clusters. Biophysical reports\| PubMed ID: 36425461	2021
Regulating DNA-Hybridization Using a Chemically Fueled Reaction Cycle. Journal of the American Chemical Society\| PubMed ID: 36442850	2022
Reconstitution of a Reversible Membrane Switch via Prenylation by One-Pot Cell-Free Expression. ACS synthetic biology\| PubMed ID: 36445320	2023
MipZ caps the plus-end of FtsZ polymers to promote their rapid disassembly. Proceedings of the National Academy of Sciences of the United States of America\| PubMed ID: 36490318	2022
Spatiotemporal Propagation of a Minimal Catalytic RNA Network in GUV Protocells by Temperature Cycling and Phase Separation. Angewandte Chemie (International ed. in English)\| PubMed ID: 36757674	2023
Periodic temperature changes drive the proliferation of self-replicating RNAs in vesicle populations. Nature communications\| PubMed ID: 36869058	2023
Structural diversity of photoswitchable sphingolipids for optodynamic control of lipid microdomains. Biophysical journal\| PubMed ID: 36869591	2023
Insights into receptor structure and dynamics at the surface of living cells. Nature communications\| PubMed ID: 36949079	2023
Binding and Characterization of DNA Origami Nanostructures on Lipid Membranes. Methods in molecular biology (Clifton, N.J.)\| PubMed ID: 37166721	2023
Protein-Based Patterning to Spatially Functionalize Biomimetic Membranes. Small methods\| PubMed ID: 37350500	2023
Biomolecular condensate drives polymerization and bundling of the bacterial tubulin FtsZ to regulate cell division. Nature communications\| PubMed ID: 37380708	2023
Dual-color DNA-PAINT single-particle tracking enables extended studies of membrane protein interactions. Nature communications\| PubMed ID: 37468504	2023
Nanoscale structural response of biomimetic cell membranes to controlled dehydration. Nanoscale\| PubMed ID: 38062887	2023
Present and future of synthetic cell development. Nature reviews. Molecular cell biology\| PubMed ID: 38102450	2024
Light-Activated Synthetic Rotary Motors in Lipid Membranes Induce Shape Changes Through Membrane Expansion. Advanced materials (Deerfield Beach, Fla.)\| PubMed ID: 38215457	2024
Protein-Assisted Large-Scale Assembly and Differential Patterning of DNA Origami Lattices. Small (Weinheim an der Bergstrasse, Germany)\| PubMed ID: 38229553	2024
Machine learning-aided design and screening of an emergent protein function in synthetic cells. Nature communications\| PubMed ID: 38443351	2024
Membrane-induced 2D phase separation of the focal adhesion protein talin. Nature communications\| PubMed ID: 38862544	2024
Measuring Partition Coefficients of In Vitro Biomolecular Condensates Using Fluorescence Correlation Spectroscopy. Methods in molecular biology (Clifton, N.J.)\| PubMed ID: 39028519	2024
Self-organized spatial targeting of contractile actomyosin rings for synthetic cell division. Nature communications\| PubMed ID: 39614082	2024
Mutual Dependence between Membrane Phase Separation and Bacterial Division Protein Dynamics in Synthetic Cell Models. Angewandte Chemie (International ed. in English)\| PubMed ID: 39623974	2025
Polyproline-Polyornithine Diblock Copolymers with Inherent Mitochondria Tropism. Advanced materials (Deerfield Beach, Fla.)\| PubMed ID: 39797465	2025
Photoswitchable Molecular Motor Phospholipid: Synthesis, Characterization, and Integration into Lipid Vesicles. Langmuir : the ACS journal of surfaces and colloids\| PubMed ID: 39900533	2025
Fluorescence correlation spectroscopy for particle sizing: A notorious challenge. Biophysical journal\| PubMed ID: 40134216	2025