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University of Science and Technology Beijing

8 ARTICLES PUBLISHED IN JoVE

Engineering

Measurement of Coherence Decay in GaMnAs Using Femtosecond Four-wave Mixing

Daniel Webber¹, Tristan de Boer¹, Murat Yildirim¹, Sam March¹, Reuble Mathew¹, Angela Gamouras¹, Xinyu Liu², Margaret Dobrowolska², Jacek Furdyna², Kimberley Hall¹

¹Department of Physics and Atmospheric Science, Dalhousie University, ²Department of Physics, University of Notre Dame

The technique of femtosecond four-wave mixing is described, including spectrally-resolved and time-resolved configurations. We illustrate the utility of this technique for the investigation of crucial physical properties in the III-V diluted magnetic semiconductors, afforded by its nonlinearity and high temporal resolution.

JoVE Journal

Synthesis of High Purity Nonsymmetric Dialkylphosphinic Acid Extractants

Junlian Wang¹, Meiying Xie¹, Xinyu Liu¹, Shengming Xu²

¹School of Civil and Resource Engineering, University of Science and Technology Beijing, ²Institute of Nuclear and New Energy Technology, Tsinghua University

A protocol for the synthesis of high purity nonsymmetric dialkylphosphinic acid extractants is presented, taking (2,3-dimethylbutyl)(2,4,4'-trimethylpentyl)phosphinic acid as an example.

JoVE Journal

Fabricating High-viscosity Droplets using Microfluidic Capillary Device with Phase-inversion Co-flow Structure

Jiang Li¹, Jia Man², Zhongnan Li², Haosheng Chen²

¹School of Mechanical Engineering, University of Science and Technology Beijing, ²State Key Laboratory of Tribology, Tsinghua University

A phase-inversion co-flow device is demonstrated to generate monodisperse high-viscosity droplets above 1 Pas, which is difficult to realize in droplet microfluidics.

Engineering

Quantitative Analysis by Thermogravimetry-Mass Spectrum Analysis for Reactions with Evolved Gases

Rongbin Li¹, Qian Huang², Kai Wei², Hongde Xia²

¹School of Metallurgical and Ecological Engineering, University of Science and Technology Beijing, ²Institute of Engineering Thermophysics, Chinese Academy of Sciences

Precise determination of the evolved gases' flow rate is key to study the details of reactions. We provide a novel quantitative analysis method of equivalent characteristic spectrum analysis for thermogravimetry-mass spectrum analysis by establishing the calibration system of the characteristic spectrum and relative sensitivity, for obtaining the flow rate.

Environment

Using Caenorhabditis elegans for Studying Trans- and Multi-Generational Effects of Toxicants

Zhuo Li^1,2, Fangting Ai³, Jing Zhang⁴, Zhenyang Yu^1,2, Daqiang Yin^1,2

¹College of Environmental Sciences and Engineering, Tongji University, ²Shanghai Institute of Pollution Control and Ecological Security, ³Jiaxing Tongji Institute of Environment, ⁴College of Ecological Technique and Engineering, Shanghai Institute of Technology

Trans- and multi-generational effects of persistent chemicals are essential in judging their long-term consequences in the environment and on the human health. We provide novel detailed methods for studying trans- and multi-generational effects using free-living nematode Caenorhabditis elegans.

Engineering

Measurement of Chladni Mode Shapes with an Optical Lever Method

Rong Feng^*1, Yan Luo^*1, Yixue Dong¹, Mengke Ma², Yuqi Wang², Jing Zhang³, Wenjiang Ma³, Donghuan Liu⁴

¹School of Materials Science and Engineering, University of Science and Technology Beijing, ²School of Civil and Resource Engineering, University of Science and Technology Beijing, ³Basic Experimental Center for Natural Science, University of Science and Technology Beijing, ⁴School of Mathematics and Physics, University of Science and Technology Beijing

A simple method of measuring the Chladni mode shape on an elastic plate by the principle of an optical lever is proposed.

Cancer Research

Studying TGF-β Signaling and TGF-β-induced Epithelial-to-mesenchymal Transition in Breast Cancer and Normal Cells

Jing Zhang¹, Midory Thorikay¹, Gerard van der Zon¹, Maarten van Dinther¹, Peter ten Dijke¹

¹Oncode Institute and Department of Cell Chemical Biology, Leiden University Medical Center

We describe a systematic workflow to investigate TGF-β signaling and TGF-β-induced EMT by studying the protein and gene expression involved in this signaling pathway. The methods include Western blotting, a luciferase reporter assay, qPCR, and immunofluorescence staining.

Cancer Research

High-Resolution Ultrasonography for the Analysis of Orthotopic ATC Tumors in a Genetically Engineered Mouse Model

Yushuang He¹, Yan Luo¹, Zhihui Li², Han Luo², Huayun Yan³, Yingfang Ma³, Xinyue Zhou²

¹Department of Ultrasound, West China Hospital, Sichuan University, ²Department of Thyroid and Parathyroid Surgery, Laboratory of Thyroid and Parathyroid Disease, Frontiers Science Center for Disease-related Molecular Network, West China Hospital, Sichuan University, ³State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University and Collaborative Innovation Center

The present protocol describes high-frequency ultrasonography for visualizing the entire mouse thyroid gland and monitoring the growth of anaplastic thyroid carcinoma.