Chapman University
2 ARTICLES PUBLISHED IN JoVE
Biology
Quantifying Agonist Activity at G Protein-coupled Receptors
Frederick J. Ehlert 1, Hinako Suga 2, Michael T. Griffin 3
1Department of Pharmacology, University of California, Irvine, 2Department of Pharmacology, University of California, 3Schmid College of Science, Chapman University
A method for estimating the affinity constant of an agonist for the active state (Kb) of a G protein-coupled receptor is described. The analysis provides absolute or relative measures of Kb depending on whether constitutive receptor activation is measurable. Our method applies to various responses downstream from receptor activation.
Neuroscience
Full- versus Sub-Regional Quantification of Amyloid-Beta Load on Mouse Brain Sections
Yuu Ohno 1, Riley Murphy 2, Matthew Choi 3, Weijun Ou 4, Rachita K. Sumbria 4,5
1Henry E. Riggs School of Applied Life Sciences, Keck Graduate Institute, 2Crean College of Health and Behavioral Sciences, Chapman University, 3Keck Science Department, Claremont McKenna College, 4Department of Biomedical and Pharmaceutical Sciences, School of Pharmacy, Chapman University, 5Department of Neurology, University of California, Irvine
The present protocol describes and compares the procedure to perform a full-region or sub-region of interest analysis of sagittal mouse brain sections to quantify amyloid-beta load in the APP/PS1 transgenic mouse model of Alzheimer's disease.
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