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Chapman University

2 ARTICLES PUBLISHED IN JoVE

Biology

Quantifying Agonist Activity at G Protein-coupled Receptors

Frederick J. Ehlert¹, Hinako Suga², Michael T. Griffin³

¹Department of Pharmacology, University of California, Irvine, ²Department of Pharmacology, University of California, ³Schmid College of Science, Chapman University

A method for estimating the affinity constant of an agonist for the active state (K_b) of a G protein-coupled receptor is described. The analysis provides absolute or relative measures of K_b depending on whether constitutive receptor activation is measurable. Our method applies to various responses downstream from receptor activation.

Neuroscience

Full- versus Sub-Regional Quantification of Amyloid-Beta Load on Mouse Brain Sections

Yuu Ohno¹, Riley Murphy², Matthew Choi³, Weijun Ou⁴, Rachita K. Sumbria^4,5

¹Henry E. Riggs School of Applied Life Sciences, Keck Graduate Institute, ²Crean College of Health and Behavioral Sciences, Chapman University, ³Keck Science Department, Claremont McKenna College, ⁴Department of Biomedical and Pharmaceutical Sciences, School of Pharmacy, Chapman University, ⁵Department of Neurology, University of California, Irvine

The present protocol describes and compares the procedure to perform a full-region or sub-region of interest analysis of sagittal mouse brain sections to quantify amyloid-beta load in the APP/PS1 transgenic mouse model of Alzheimer's disease.