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Copenhagen University Hospital

5 ARTICLES PUBLISHED IN JoVE

Neuroscience

Autoradiography as a Simple and Powerful Method for Visualization and Characterization of Pharmacological Targets

Nane Griem-Krey¹, Anders Bue Klein¹, Matthias Herth^1,2,3, Petrine Wellendorph¹

¹Department of Drug Design and Pharmacology, Faculty of Health and Medical Sciences, University of Copenhagen, ²Neurobiology Research Unit and CIMBI, Copenhagen University Hospital, ³Department of Clinical Physiology, Nuclear Medicine & PET, Copenhagen University Hospital

The method of autoradiography is routinely used to study binding of radioligands to tissue sections for determination of qualitative or quantitative pharmacology.

Biology

Medium-throughput Screening Assays for Assessment of Effects on Ca²⁺-Signaling and Acrosome Reaction in Human Sperm

Anders Rehfeld^1,2, Dorte Louise Egeberg Palme^1,2, Kristian Almstrup^1,2, Anders Juul^1,2, Niels Erik Skakkebaek^1,2

¹Department of Growth and Reproduction, Copenhagen University Hospital, ²International Center for Research and Research Training in Endocrine Disruption of Male Reproduction and Child Health (EDMaRC), University of Copenhagen

Here, two medium-throughput assays for assessment of effects on Ca²⁺-signaling and acrosome reaction in human sperm are described. These assays can be used to quickly and easily screen large amounts of compounds for effects on Ca²⁺-signaling and acrosome reaction in human sperm.

Medicine

Intravascular Lithotripsy-Assisted Transfemoral Transcatheter Aortic Valve Implantation

Philippe Nuyens¹, Ivan Wong¹, Maarten Vanhaverbeke¹, Xi Wang¹, Gintautas Bieliauskas¹, Lars Sondergaard¹, Ole De Backer¹

¹The Heart Center, Rigshospitalet, Copenhagen University Hospital

Transcatheter aortic valve implantation (TAVI) has been shown to generate the best clinical outcomes when performed by the percutaneous transfemoral approach. Intravascular lithotripsy (IVL) can facilitate a transfemoral process in patients with calcified iliofemoral vascular disease and borderline intraluminal diameters. The present protocol describes IVL-assisted transfemoral TAVI.

Medicine

Using the Endoscope for Endobronchial Ultrasound in the Esophagus

Mohammad A. Issa¹, Paul F. Clementsen², Christian B. Laursen^3,4, Peter Vilmann^5,6, Ida S. Christiansen⁷, Laurence Crombag⁸, Uffe Bodtger^1,9

¹Pulmonary Research Unit (PLUZ), Department of Respiratory Medicine, Zealand University Hospital, ²Copenhagen Academy for Medical Education and Simulation (CAMES), Copenhagen University Hospital, Rigshospitalet, ³Department of Respiratory Medicine, Odense University Hospital, ⁴Odense Respiratory Research Unit (ODIN), Department of Clinical Research, University of Southern Denmark, ⁵Department of Surgery, Copenhagen University Hospital, ⁶Department of Clinical Medicine, University of Copenhagen, ⁷Department of Pathology, Copenhagen University Hospital, Rigshospitalet, ⁸Department of Respiratory Medicine, University Medical Center, University of Amsterdam, ⁹Institute for Regional Health Research, University of Southern Denmark

Transesophageal ultrasound (EUS-B) is a safe and feasible procedure using the echoendobronchoscope (EBUS) in esophagus and stomach. After identifying six anatomical landmarks, additional structures can be identified and biopsied, sparing subsequent diagnostic sessions. Thus, EUS-B is an ideal continuation of bronchoscopy and EBUS in diagnosing lung cancer and other diseases.

Medicine

Doppler Ultrasound-Based Leg Blood Flow Assessment During Single-Leg Knee-Extensor Exercise in an Uncontrolled Setting

Jacob Peter Hartmann^1,2,3, Rikke Krabek¹, Stine B. Nymand^1,3, Helene Hartmeyer¹, Lasse Gliemann⁴, Ronan M. G. Berg^1,2,3,5, Ulrik Winning Iepsen^1,6

¹Centre for Physical Activity Research, Copenhagen University Hospital, ²Department of Clinical Physiology and Nuclear Medicine, Copenhagen University Hospital, ³Department of Biomedical Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, ⁴Department of Nutrition, Exercise and Sports, Integrated Physiology Group, ⁵Neurovascular Research Laboratory, Faculty of Life Sciences and Education, University of South Wales, ⁶Department of Anaesthesiology and Intensive Care, Copenhagen University Hospital, Hvidovre Hospital

This test-retest study evaluated leg blood flow measured by the Doppler ultrasound technique during single-leg knee-extensor exercise. The within-day, between-day, and inter-rater reliability of the method was investigated. The approach demonstrated high within-day and acceptable between-day reliability. However, the inter-rater reliability was unacceptably low during rest and at low workloads.