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Copenhagen University Hospital

5 ARTICLES PUBLISHED IN JoVE

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Neuroscience

Autoradiography as a Simple and Powerful Method for Visualization and Characterization of Pharmacological Targets
Nane Griem-Krey 1, Anders Bue Klein 1, Matthias Herth 1,2,3, Petrine Wellendorph 1
1Department of Drug Design and Pharmacology, Faculty of Health and Medical Sciences, University of Copenhagen, 2Neurobiology Research Unit and CIMBI, Copenhagen University Hospital, 3Department of Clinical Physiology, Nuclear Medicine & PET, Copenhagen University Hospital

The method of autoradiography is routinely used to study binding of radioligands to tissue sections for determination of qualitative or quantitative pharmacology.

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Biology

Medium-throughput Screening Assays for Assessment of Effects on Ca2+-Signaling and Acrosome Reaction in Human Sperm
Anders Rehfeld 1,2, Dorte Louise Egeberg Palme 1,2, Kristian Almstrup 1,2, Anders Juul 1,2, Niels Erik Skakkebaek 1,2
1Department of Growth and Reproduction, Copenhagen University Hospital, 2International Center for Research and Research Training in Endocrine Disruption of Male Reproduction and Child Health (EDMaRC), University of Copenhagen

Here, two medium-throughput assays for assessment of effects on Ca2+-signaling and acrosome reaction in human sperm are described. These assays can be used to quickly and easily screen large amounts of compounds for effects on Ca2+-signaling and acrosome reaction in human sperm.

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Medicine

Intravascular Lithotripsy-Assisted Transfemoral Transcatheter Aortic Valve Implantation
Philippe Nuyens 1, Ivan Wong 1, Maarten Vanhaverbeke 1, Xi Wang 1, Gintautas Bieliauskas 1, Lars Sondergaard 1, Ole De Backer 1
1The Heart Center, Rigshospitalet, Copenhagen University Hospital

Transcatheter aortic valve implantation (TAVI) has been shown to generate the best clinical outcomes when performed by the percutaneous transfemoral approach. Intravascular lithotripsy (IVL) can facilitate a transfemoral process in patients with calcified iliofemoral vascular disease and borderline intraluminal diameters. The present protocol describes IVL-assisted transfemoral TAVI.

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Medicine

Using the Endoscope for Endobronchial Ultrasound in the Esophagus
Mohammad A. Issa 1, Paul F. Clementsen 2, Christian B. Laursen 3,4, Peter Vilmann 5,6, Ida S. Christiansen 7, Laurence Crombag 8, Uffe Bodtger 1,9
1Pulmonary Research Unit (PLUZ), Department of Respiratory Medicine, Zealand University Hospital, 2Copenhagen Academy for Medical Education and Simulation (CAMES), Copenhagen University Hospital, Rigshospitalet, 3Department of Respiratory Medicine, Odense University Hospital, 4Odense Respiratory Research Unit (ODIN), Department of Clinical Research, University of Southern Denmark, 5Department of Surgery, Copenhagen University Hospital, 6Department of Clinical Medicine, University of Copenhagen, 7Department of Pathology, Copenhagen University Hospital, Rigshospitalet, 8Department of Respiratory Medicine, University Medical Center, University of Amsterdam, 9Institute for Regional Health Research, University of Southern Denmark

Transesophageal ultrasound (EUS-B) is a safe and feasible procedure using the echoendobronchoscope (EBUS) in esophagus and stomach. After identifying six anatomical landmarks, additional structures can be identified and biopsied, sparing subsequent diagnostic sessions. Thus, EUS-B is an ideal continuation of bronchoscopy and EBUS in diagnosing lung cancer and other diseases.

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Medicine

Doppler Ultrasound-Based Leg Blood Flow Assessment During Single-Leg Knee-Extensor Exercise in an Uncontrolled Setting
Jacob Peter Hartmann 1,2,3, Rikke Krabek 1, Stine B. Nymand 1,3, Helene Hartmeyer 1, Lasse Gliemann 4, Ronan M. G. Berg 1,2,3,5, Ulrik Winning Iepsen 1,6
1Centre for Physical Activity Research, Copenhagen University Hospital, 2Department of Clinical Physiology and Nuclear Medicine, Copenhagen University Hospital, 3Department of Biomedical Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, 4Department of Nutrition, Exercise and Sports, Integrated Physiology Group, 5Neurovascular Research Laboratory, Faculty of Life Sciences and Education, University of South Wales, 6Department of Anaesthesiology and Intensive Care, Copenhagen University Hospital, Hvidovre Hospital

This test-retest study evaluated leg blood flow measured by the Doppler ultrasound technique during single-leg knee-extensor exercise. The within-day, between-day, and inter-rater reliability of the method was investigated. The approach demonstrated high within-day and acceptable between-day reliability. However, the inter-rater reliability was unacceptably low during rest and at low workloads.

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