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Tecnologico de Monterrey

4 ARTICLES PUBLISHED IN JoVE

Engineering

Fabrication of 3D Carbon Microelectromechanical Systems (C-MEMS)

Bidhan Pramanick¹, Sergio O. Martinez-Chapa¹, Marc Madou^1,2, Hyundoo Hwang^1,3

¹School of Engineering and Sciences, Tecnologico de Monterrey, ²Department of Mechanical and Aerospace Engineering, University of California, ³BBB Inc

Long and hollow glassy carbon microfibers were fabricated based on the pyrolysis of a natural product, human hair. The two fabrication steps of carbon microelectromechanical and carbon nanoelectromechanical systems, or C-MEMS and C-NEMS, are: (i) photolithography of a carbon-rich polymer precursor and (ii) pyrolysis of the patterned polymer precursor.

Neuroscience

Three-Dimensional Motor Nerve Organoid Generation

Tatsuya Osaki^1,2, Siu Yu A. Chow^1,2, Yui Nakanishi^1,2, Joel Hernández^1,3, Jiro Kawada⁴, Teruo Fujii¹, Yoshiho Ikeuchi^1,2

¹Institute of Industrial Science, The University of Tokyo, ²Department of Chemistry and Biotechnology, School of Engineering, The University of Tokyo, ³Faculty of Science and Engineering, Tecnologico de Monterrey, ⁴Jiksak Bioengineering, Inc.

This protocol provides a comprehensive procedure to fabricate human iPS cell-derived motor nerve organoid through spontaneous assembly of a robust bundle of axons extended from a spheroid in a tissue culture chip.

Neuroscience

Robust Tissue Fabrication for Long-Term Culture of iPSC-Derived Brain Organoids for Aging Research

Lena Sophie Koch¹, David Choy Buentello^2,3, Kerensa Broersen¹

¹Department of Applied Stem Cell Technologies, TechMed Centre Enschede, University of Twente, ²Centro de Biotecnología-FEMSA, Tecnologico de Monterrey, ³Department of Genetics, Harvard Medical School

The present protocol provides a step-by-step procedure for the reproducible generation, maintenance, and aging of cerebral organoids derived from human-induced pluripotent stem cells (iPSCs). This method enables culturing and maturing cerebral organoids for extended periods, which facilitates the modeling of processes involved in brain aging and age-related pathogenesis.

JoVE Core

Analysis of the Mitochondrial Density and Longitudinal Distribution in Rat Live-Skeletal Muscle Fibers by Confocal Microscopy

Perla Pérez-Treviño¹, Bianca Nieblas^1,2, Selma Romina López-Vaquera¹, Noemí García^1,2,3

¹Experimental Medicine and Advanced Therapies, The Institute for Obesity Research, Tecnologico de Monterrey, ²Escuela de Medicina y Ciencias de la Salud, Tecnologico de Monterrey, ³Preclinical Research Unit, Tecnologico de Monterrey

Here, we present a protocol to analyze changes in mitochondrial density and longitudinal distribution by live-skeletal muscle imaging using confocal microscopy for mitochondrial network scanning.