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Zhejiang Academy of Agricultural Sciences

3 ARTICLES PUBLISHED IN JoVE

Environment

Generating Homo- and Heterografts Between Watermelon and Bottle Gourd for the Study of Cold-responsive MicroRNAs

Lingping Wang^*1, Xinyi Wu^*1, Guojing Li^1,2, Xiaohua Wu¹, Dehui Qin³, Ye Tao⁴, Pei Xu^1,2

¹Institute of Vegetables, Zhejiang Academy of Agricultural Sciences, ²State Key Lab Breeding Base for Sustainable Control of Plant Pest and Disease, Zhejiang Academy of Agricultural Sciences, ³Institute of Horticulture, Zhejiang Academy of Agricultural Sciences, ⁴Shanghai Biozeron Biotechnology Co., Ltd

Here we present a detailed protocol for efficiently making homo- and heterografts between watermelon and bottle gourd, in addition to methods of tissue sampling, data generation, and data analysis, for the investigation of cold-responsive microRNAs.

Biology

Profiling of H3K4me3 Modification in Plants using Cleavage under Targets and Tagmentation

Xiaoyuan Tao^*1,2, Mengtao Gao^*3, Siyuan Wang^*1, Xueying Guan^1,4

¹College of Agriculture and Biotechnology, Zhejiang University, ²Central Laboratory, State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Zhejiang Academy of Agricultural Sciences, ³State Key Laboratory of Crop Genetics and Germplasm Enhancement, Cotton Hybrid R & D Engineering Center (the Ministry of Education), College of Agriculture, Nanjing Agricultural University, ⁴Hainan Institute of Zhejiang University

Cleavage under targets and tagmentation (CUT&Tag) is an efficient chromatin epigenomic profiling strategy. This protocol presents a refined CUT&Tag strategy for the profiling of histone modifications in plants.

Biology

Screening of Tobacco Genotypes for Phytophthora nicotianae Resistance

Yutong Liu¹, Mingming Sun¹, Zipeng Jiang¹, Xinfang Wang², Bingguang Xiao³, Aiguo Yang¹, He Meng¹, Lirui Cheng¹

¹Key Laboratory of Tobacco Genetic Improvement and Biotechnology, Tobacco Research Institute, Chinese Academy of Agricultural Sciences, ²Institute of Rural Development, Zhejiang Academy of Agricultural Sciences, ³Yunnan Academy of Tobacco Agricultural Sciences

Here, a protocol is presented for the efficient and accurate screening of tobacco genotypes for Phytophthora nicotianae resistance in seedlings. This is a practical approach for precision breeding, as well as molecular mechanism research.