Université de Reims Champagne-Ardenne
2 ARTICLES PUBLISHED IN JoVE
Biochemistry
Measuring Interactions between Fluorescent Probes and Lignin in Plant Sections by sFLIM Based on Native Autofluorescence
Christine Terryn 1, Anouck Habrant 2, Gabriel Paës 2, Corentin Spriet 3
1PICT Platform, Université de Reims Champagne-Ardenne, 2Fractionation of AgroResources and Environment (FARE) Laboratory, INRA, Université de Reims Champagne-Ardenne, 3Unité de Glycobiologie Structurale et Fonctionnelle, Université de Lille
This protocol describes an original setup combining spectral and fluorescence lifetime measurements to evaluate Förster resonance energy transfer (FRET) between rhodamine-based fluorescent probes and lignin polymer directly in thick plant sections.
Biology
An Innovative 3D-Printed Insert Designed to Enable Straightforward 2D and 3D Cell Cultures
Charlie Colin-Pierre 1,2,3, Oussama El Baraka 3, Laurent Ramont 1,2,4, Stéphane Brézillon 1,2
1Laboratoire de Biochimie Médicale et Biologie Moléculaire, Université de Reims Champagne-Ardenne, 2UMR CNRS 7369, Matrice Extracellulaire et Dynamique Cellulaire-MEDyC, Université de Reims Champagne-Ardenne Reims, 3BASF Beauty Care Solutions, 4Service Biochimie-Pharmacologie-Toxicologie, Centre Hospitalier Universitaire (CHU) de Reims
In this paper, a newly designed 3D-printed insert is presented as a model of co-culture and validated through the study of the paracrine intercellular communication between endothelial cells and keratinocytes.
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