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Université de Reims Champagne-Ardenne

2 ARTICLES PUBLISHED IN JoVE

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Biochemistry

Measuring Interactions between Fluorescent Probes and Lignin in Plant Sections by sFLIM Based on Native Autofluorescence
Christine Terryn 1, Anouck Habrant 2, Gabriel Paës 2, Corentin Spriet 3
1PICT Platform, Université de Reims Champagne-Ardenne, 2Fractionation of AgroResources and Environment (FARE) Laboratory, INRA, Université de Reims Champagne-Ardenne, 3Unité de Glycobiologie Structurale et Fonctionnelle, Université de Lille

This protocol describes an original setup combining spectral and fluorescence lifetime measurements to evaluate Förster resonance energy transfer (FRET) between rhodamine-based fluorescent probes and lignin polymer directly in thick plant sections.

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Biology

An Innovative 3D-Printed Insert Designed to Enable Straightforward 2D and 3D Cell Cultures
Charlie Colin-Pierre 1,2,3, Oussama El Baraka 3, Laurent Ramont 1,2,4, Stéphane Brézillon 1,2
1Laboratoire de Biochimie Médicale et Biologie Moléculaire, Université de Reims Champagne-Ardenne, 2UMR CNRS 7369, Matrice Extracellulaire et Dynamique Cellulaire-MEDyC, Université de Reims Champagne-Ardenne Reims, 3BASF Beauty Care Solutions, 4Service Biochimie-Pharmacologie-Toxicologie, Centre Hospitalier Universitaire (CHU) de Reims

In this paper, a newly designed 3D-printed insert is presented as a model of co-culture and validated through the study of the paracrine intercellular communication between endothelial cells and keratinocytes.

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