Accedi

Université de Reims Champagne-Ardenne

2 ARTICLES PUBLISHED IN JoVE

Biochemistry

Measuring Interactions between Fluorescent Probes and Lignin in Plant Sections by sFLIM Based on Native Autofluorescence

Christine Terryn¹, Anouck Habrant², Gabriel Paës², Corentin Spriet³

¹PICT Platform, Université de Reims Champagne-Ardenne, ²Fractionation of AgroResources and Environment (FARE) Laboratory, INRA, Université de Reims Champagne-Ardenne, ³Unité de Glycobiologie Structurale et Fonctionnelle, Université de Lille

This protocol describes an original setup combining spectral and fluorescence lifetime measurements to evaluate Förster resonance energy transfer (FRET) between rhodamine-based fluorescent probes and lignin polymer directly in thick plant sections.

Biology

An Innovative 3D-Printed Insert Designed to Enable Straightforward 2D and 3D Cell Cultures

Charlie Colin-Pierre^1,2,3, Oussama El Baraka³, Laurent Ramont^1,2,4, Stéphane Brézillon^1,2

¹Laboratoire de Biochimie Médicale et Biologie Moléculaire, Université de Reims Champagne-Ardenne, ²UMR CNRS 7369, Matrice Extracellulaire et Dynamique Cellulaire-MEDyC, Université de Reims Champagne-Ardenne Reims, ³BASF Beauty Care Solutions, ⁴Service Biochimie-Pharmacologie-Toxicologie, Centre Hospitalier Universitaire (CHU) de Reims

In this paper, a newly designed 3D-printed insert is presented as a model of co-culture and validated through the study of the paracrine intercellular communication between endothelial cells and keratinocytes.