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the First Affiliated Hospital of Jinan University

4 ARTICLES PUBLISHED IN JoVE

Medicine

Intratracheal Administration of Dry Powder Formulation in Mice

Yingshan Qiu¹, Qiuying Liao¹, Michael Y.T. Chow^1,2, Jenny K.W. Lam¹

¹Department of Pharmacology and Pharmacy, LKS Faculty of Medicine, The University of Hong Kong, ²Advanced Drug Delivery Group, Sydney Pharmacy School, Faculty of Medicine and Health, The University of Sydney

Dry powder formulations for inhalation have great potential in treating respiratory diseases. Before entering human studies, it is necessary to evaluate the efficacy of the dry powder formulation in preclinical studies. A simple and noninvasive method of the administration of dry powder in mice through the intratracheal route is presented.

Medicine

A Surgical Model of Heart Failure with Preserved Ejection Fraction in Tibetan Minipigs

Xiaohui Li^*1,2, Weijiang Tan^*1,3, Xiang Li^*1, Shuang Zheng^*1, Xiaoshen Zhang², Honghua Chen¹, Zhanhong Pan¹, Caiyi Zhu¹, Feng Hua Yang¹

¹Guangdong Laboratory Animals Monitoring Institute, ²Department of Cardiovascular Surgery, the First Affiliated Hospital, Jinan University, ³College of Veterinary Medicine, South China Agricultural University

The present protocol describes a step-by-step procedure to establish a minipig model of heart failure with preserved ejection fraction using descending aortic constriction. The methods for evaluating cardiac morphology, histology, and function of this disease model are also presented.

Medicine

Establishment and Evaluation of a Porcine Vein Graft Disease Model

Xiaohui Li^*1, Jinlin Hu^*1, Weijiang Tan^*2, Zhaoming Lin^*1, Caiyi Zhu², Chengfeng Huang¹, Jiawen Huang³, Yintian Liu¹, Qiuying Liao¹, Hua Lu¹, Xiaoshen Zhang¹

¹Department of Cardiovascular Surgery, the First Affiliated Hospital of Jinan University, ²Guangdong Provincial Key Laboratory of Laboratory Animals, Guangdong Laboratory Animals Monitoring Institute, ³The First Affiliated Hospital of Jinan University

In this protocol, novel pig vein bypass grafting was performed through a small incision in the left chest wall without cardiopulmonary bypass. A postoperative pathology study was done, which showed intimal thickening.

Genetics

Investigating the Pathogenesis of MYH7 Mutation Gly823Glu in Familial Hypertrophic Cardiomyopathy using a Mouse Model

Yu Xia^*1,2, Jinlin Hu^*3, Xiang Li⁴, Shuang Zheng⁴, Ge Wang^1,2, Songtao Tan^1,2, Zengxiao Zou^1,2, Qiong Ling^2,5, Fenghua Yang⁴, Xiaoping Fan^1,2

¹Department of Cardiovascular Surgery, Guangdong Provincial Hospital of Traditional Chinese Medicine, the Second Affiliated Hospital of Guangzhou University of Chinese Medicine, ²The Second Clinical College of Guangzhou University of Chinese Medicine, ³Department of Cardiovascular Surgery, The First Affiliated Hospital, Jinan University, ⁴Guangdong Provincial Key Laboratory of Laboratory Animals, Guangdong Laboratory Animals Monitoring Institute, ⁵Department of Anesthesiology, Guangdong Provincial Hospital of Chinese Medicine, the Second Affiliated Hospital of Guangzhou University of Chinese Medicine

Based on the familial hereditary cardiomyopathy family found in our clinical work, we created a C57BL/6N mouse model with a point mutation (G823E) at the mouse MYH7 locus through CRISPR/Cas9-mediated genome engineering to verify this mutation.