Bölüm 33
Hücreleri, Dokuları ve Molekülleri Görselleştirmek
Biyolojik Örneklerin Optik Mikroskopi ile Görüntülenmesi
Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in ...
Faz Kontrastı ve Diferansiyel Girişim Kontrast Mikroskobu
Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular ...
Fiksasyon ve Kesit Alma
Two basic types of preparation are used to visualize specimens with a light microscope: wet mounts and fixed specimens.
The simplest type of preparation ...
İmmünofloresan Mikroskobu
A fluorescence microscope uses fluorescent chromophores called fluorochromes, which can absorb energy from a light source and then emit this energy as ...
İmmünositokimya ve İmmünohistokimya
Immunocytochemistry (ICC) and immunohistochemistry (IHC) are techniques that use antibodies to check for specific proteins or antigens in a sample. The ...
Konfokal Floresan Mikroskobu
Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability ...
Canlı Hücrelerde Protein Dinamiği
Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent ...
Toplam İç Yansıma Floresan Mikroskobu
Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid ...
Atomik Kuvvet Mikroskobu
Atomic force microscopy (AFM) is a type of scanning probe microscopy that can analyze topographic details of various specimens like ceramics, glass, ...
Süper Çözünürlüklü Floresan Mikroskobu
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread ...
Elektron mikroskobuna genel bakış
The wavelengths of visible light ultimately limit the maximum theoretical resolution of images created by light microscopes. Most light microscopes can ...
Taramalı Elektron Mikroskobu
A scanning electron microscope (SEM) is used to study the surface features of a sample by using an electron beam that scans the sample surface in a ...
Transmisyon Elektron Mikroskobu
In 1931, physicist Ernst Ruska—building on the idea that magnetic fields can direct an electron beam just as lenses can direct a beam of light in an ...
Elektron mikroskobu için numunelerin hazırlanması
To be visualized by an electron microscope, either transmission or scanning, biological samples need to be fixed (stabilized) so the electron beam does ...
İmmünogold Elektron Mikroskobu
Immunoelectron microscopy utilizes immunogold labeling of endogenous proteins with specific antibodies to detect and localize these proteins in cells and ...
Kriyo-elektron mikroskobu
Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological ...
Elektron Mikroskobu Tomografisi ve Tek Parçacık Rekonstrüksiyonu
Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron ...
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