Capítulo 33
Visualização de Células, Tecidos e Moléculas
Imageamento de Amostras Biológicas com Microscopia Óptica
Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in ...
Contraste de Fase e Microscopia de Contraste de Interferência Diferencial
Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular ...
Fixação eSseccionamento
Two basic types of preparation are used to visualize specimens with a light microscope: wet mounts and fixed specimens.
The simplest type of preparation ...
Microscopia de Imunofluorescência
A fluorescence microscope uses fluorescent chromophores called fluorochromes, which can absorb energy from a light source and then emit this energy as ...
Imunocitoquímica e Imunohistoquímica
Immunocytochemistry (ICC) and immunohistochemistry (IHC) are techniques that use antibodies to check for specific proteins or antigens in a sample. The ...
Microscopia de Fluorescência Confocal
Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability ...
Dinâmica de Proteínas em Células Vivas
Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent ...
Microscopia de Fluorescência de Reflexão Interna Total
Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid ...
Microscopia de Força Atômica
Atomic force microscopy (AFM) is a type of scanning probe microscopy that can analyze topographic details of various specimens like ceramics, glass, ...
Microscopia de Fluorescência de Super-Resolução
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread ...
Visão Geral da Microscopia Eletrônica
The wavelengths of visible light ultimately limit the maximum theoretical resolution of images created by light microscopes. Most light microscopes can ...
Microscopia Eletrônica de Varredura
A scanning electron microscope (SEM) is used to study the surface features of a sample by using an electron beam that scans the sample surface in a ...
Microscopia Eletrônica de Transmissão
In 1931, physicist Ernst Ruska—building on the idea that magnetic fields can direct an electron beam just as lenses can direct a beam of light in an ...
Preparação de Amostras para Microscopia Eletrônica
To be visualized by an electron microscope, either transmission or scanning, biological samples need to be fixed (stabilized) so the electron beam does ...
Microscopia Eletrônica Immunogold
Immunoelectron microscopy utilizes immunogold labeling of endogenous proteins with specific antibodies to detect and localize these proteins in cells and ...
Crio-microscopia Eletrônica
Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological ...
Tomografia Microscópica Eletrônica e Reconstrução de Partículas Individuais
Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron ...
Copyright © 2025 MyJoVE Corporation. Todos os direitos reservados