Chapter 33
Visualizing Cells, Tissues, and Molecules
Optical Microscopy를 사용한 생물학적 시료 이미징
Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in ...
Phase Contrast and Differential Interference Contrast Microscopy
Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular ...
정착과 절편
Two basic types of preparation are used to visualize specimens with a light microscope: wet mounts and fixed specimens.
The simplest type of preparation ...
면역형광 현미경 검사
A fluorescence microscope uses fluorescent chromophores called fluorochromes, which can absorb energy from a light source and then emit this energy as ...
면역세포화학(Immunocytochemistry) 및 면역조직화학(Immunohistochemistry)
Immunocytochemistry (ICC) and immunohistochemistry (IHC) are techniques that use antibodies to check for specific proteins or antigens in a sample. The ...
컨포칼 형광 현미경 검사
Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability ...
살아있는 세포의 단백질 역학
Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent ...
Total Internal Reflection 형광 현미경 검사
Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid ...
원자력 현미경
Atomic force microscopy (AFM) is a type of scanning probe microscopy that can analyze topographic details of various specimens like ceramics, glass, ...
Super-resolution Fluorescence Microscopy
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread ...
전자 현미경 개요
The wavelengths of visible light ultimately limit the maximum theoretical resolution of images created by light microscopes. Most light microscopes can ...
주사 전자 현미경
A scanning electron microscope (SEM) is used to study the surface features of a sample by using an electron beam that scans the sample surface in a ...
투과 전자 현미경
In 1931, physicist Ernst Ruska—building on the idea that magnetic fields can direct an electron beam just as lenses can direct a beam of light in an ...
전자 현미경을 위한 시료 준비
To be visualized by an electron microscope, either transmission or scanning, biological samples need to be fixed (stabilized) so the electron beam does ...
Immunogold 전자 현미경
Immunoelectron microscopy utilizes immunogold labeling of endogenous proteins with specific antibodies to detect and localize these proteins in cells and ...
초저온 전자 현미경
Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological ...
전자 현미경 단층 촬영 및 단일 입자 재구성
Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron ...
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