The aim of the following methodologies is to facilitate and standardize attempts at assessing so transmitted helmuth infection status, and whether so transmitted helmuth infections have an effect on the physical fitness of school children. This is achieved by using the Cato Katz technique to diagnose infections of common soil transmitted helmets, namely Ascaris Lumoid TUS Tria, and the hookworms and CLIs stoma Duo Denali and NTO ERUs. As a second step, the Erman technique is performed, which is the most widely employed method for the diagnosis of Strully Stir Corrales.
Next, in order to assess the physical fitness of children, the 20 meter shuttle run test is conducted. Results are obtained that show the differences in physical fitness levels between Helmut infected and non-infected children based on the above-mentioned methodologies. A combination of these methods can help answer key questions such as whether soy transmitted helmet infections have an impact on the physical fitness of infected individuals, and what is the true burden of such infections.
The Cato Catz technique is employed to facilitate detection of parasite eggs that infected subjects pass in their feces. Begin by placing a standard Cato CATT template in the center of a microscope slide. The template has a hole in the middle for holding 41.7 milligrams of stool wearing gloves.
Scoop two to three grams of a fresh fecal sample onto a piece of newspaper or aluminum foil. Then press a piece of wire or plastic mesh on top of the feces in order to sieve the sample. Using a small plastic spatula, scrape the sieved material of the mesh and completely fill the punch hole in the Cato Kat's template with it.
Level the content of the hole with the spatula in order to remove any excess fecal material. Then remove the template vertically without disturbing the fecal material that is now adhering to the microscope slide. The template and spatula can be reused after cleaning with detergent and water.
Next place a piece of cellophane that has been soaked in glycerin malachite green solution for at least 24 hours over the fecal sample on the microscope slide. To spread the feces into a thick smear, gently press a clean microscope slide against the slide containing the fecal sample and evenly distribute the material within a circle of a diameter, slightly smaller than the breadth of the microscope slide. Now keep the slide away from direct sunlight and allow it to clear for 30 to 60 minutes.
After 30 to 60 minutes, recover the slide and systematically examine the thick smear under a light microscope at 40 to 100 times magnification. Count the number of eggs and stratify them by species. Now, multiply the egg count by 24 in order to get a standardized estimate of infection, which is conventionally expressed as number of eggs per gram of stool or EPG.
In order to perform the erman technique first set up a stand that can hold a glass funnel. Take the glass funnel and attach a piece of rubber tubing to its stem. Close the tubing with a clamp or spring clip, and then support the funnel on the stand.
Next place a sieve or a piece of wire screen shaped like a cone in the funnel. Layer this screen with a piece of medical gauze. Then fill the funnel up with tap water and ensure that the setup does not leak wearing gloves.
Scoop 20 to 30 grams of fresh stool into the middle of the medical gauze and make sure it is completely submerged in water, adding more water if necessary. Then fold the medical gaze over the sample. Next, shine artificial light at the funnel from below for two hours.
This is because the erman technique works on the principle that tests stir corrales larvae a photo tactic and will thus migrate out of an illuminated fecal sample. After two hours, gently release the clip in the rubber tubing and drain 50 milliliters of the lowest portion of the water in the funnel into a centrifuge tube. Next, centrifuge the collected water at 600 times G for five minutes.
Then carefully pour out the supernatant without disturbing the sediment. Retain the last few drops in the tube, resuspend and pipette two drops of the solution onto a microscope slide. Examine the slide under a light microscope first at 40 times magnification for parasite detection, and then at 100 to 400 times the species confirmation.
First stage larvae of OID stires are alive and she'll be actively moving under the microscope to set up the shuttle run test. First, measure out a 20 meter flat straight running course and mark the distance with cones. Place a heart rate monitor on the wrist of the test participant and the corresponding transmitter around the participant's chest.
This equipment measures heart rate before and after the test, and therefore monitors whether the participant has exerted maximal effort. After securing the monitors, ask the child to run back and forth on the course by following the pace of prerecorded sound signals that correspond to 20 meter laps start with setting a running speed of 8.5 kilometers per hour. The frequency of the signals that indicate 20 meter intervals automatically increases by an equivalent of 0.5 kilometers per hour every minute ensure that the participant starts running a new 20 meter interval.
Each time the signal is sounded and is finished before the next signal arrives. Stop the child if he or she fails to follow the pace for two consecutive 20 meter intervals. Next, record the running speed for the last interval.
The participant completed fully along with the participant's age, gender, and heart rate achieved. At the end of the test, make sure this heart rate is the child's maximal heart rate. If the maximal heart rate is not reached, the child may not have exerted maximal effort and the test should be repeated.
Now, estimate the maximum aerobic capacity or VO two max of the participant by the following equation. VO two max equals 31.025 plus 3.238 times the speeding kilometers per hour minus 3.248 times aging years plus 0.1536 times speeding kilometers per hour times aging years. The following two images depict the eggs of as scarce lumoid, which are approximately 45 to 74 microns long and 35 to 50 microns wide and have thick shell walls shown here as a fertilized a lumoid e egg.
Note the appearance of the Alta albumins coat, which gives it a golden brown appearance. In contrast, a non fertilized a lumoid e egg is shown here. Note the absences of the outer coat and that the egg is slightly elongated and larger than the fertilized egg.
Overall, this image shows the eggs of tea trico. There are approximately 50 microns long and 22 microns wide and are elongated with distinct polar plugs. In contrast, hookworm eggs seen here have a very thin wall, followed by a clear ring around a dense cluster of cells in the center of the egg.
S stir Corrales L one larvae are shown in this image. They are approximately 180 to 380 microns long and have a very short buccal cavity. A distinct genital primordial can be observed and is highlighted by the blue arrow.
The red arrow shows the tail of the larvae, which has a sharp end. The 20 meter shuttle run test was done in order to measure physical fitness. Interpretation of shuttle run test results can be performed in various ways.
The physical fitness of children can be compared between infected and non-infected subjects who are similar in terms of age and sex, other potentially relevant health conditions, socioeconomic status, and cultural habits. One can further try to identify a possible dose response relationship between the intensity of STH infections and physical fitness and compare different levels of multi parasitism with physical fitness. As an example, the graph here shows that physical fitness was significantly reduced in children infected with T Tri Coria as compared to children who were not infected with T Tri Coia.
After watching this video, you should have a good understanding of how to conduct the cut cuts technique, the bare mount technique, and the 20 meter shutter run test. These methods will help to assess whether soy transmitter helmet infections have an effect on the physical fitness of school aged children.
