Name: chemotherapy-induced vascular toxicity - real-time in vivo imaging of vessel impairment
Uploaded: 2015-01-07T13:00:00.000+00:00
Duration: 4 min 48 s
Description: Watch this Scientific Journal Video about Chemotherapy-induced Vascular Toxicity - Real-time In vivo Imaging of Vessel Impairment at JoVE.com

Etik deyimi: Bütün deneyler Kurumsal Hayvan Bakım ve Kullanım Kurulu tarafından kabul edildi. Hayvan bakımı kurumsal kurallarına göre oldu. (- 8 hafta eski 7, 25-30 gr) ICR dişi fareler Tel-Aviv Üniversitesi Sackler Tıp Fakültesi klimalı, ışık kontrollü hayvan tesislerinde muhafaza edildi. Vadede, hayvanlar anestezi doz aşımı ile ötenazi edildi. 1. lifli Konfokal Floresan Mikroskobu (FCFM) Kalibrasyon <ol><li> Aygıtı AÇIN. </li><li> Mikroprob (mini0 / 30) bağlayın. </li><li> Üreticinin talimatlarına göre cihazı kalibre edin. </li></ol> 2. Fareler Görüntüleme Hazırlık <ol><li> Her iki Ketaset (100 mg / kg) ve xyl-M2 (6 mg / kg) deri altından enjeksiyonu ile anestezi. Ayak-tutam tepkisizlik tarafından uygun anesthetization onaylayın. </li><li> Sırayla için kasık altındaki cildi insizyon<html> femoral arter damarları ortaya. Kesi aşağıdaki tuzlu su ile nemli kesi sitesini tutun. </li><li> Yaklaşık 30 saniye için, ılık su (çok sıcak dokunma) ile dolu bir torba (ya da bir eldiven) ile kuyruk ısıtın. Tuz veya kemoterapötik ajanın ya kuyruk damarına bir iğne (30 g, 1/2 inç) sokulması ve buna 1 ml şırınga takılarak FITC dekstran (kontrast bir madde) verilmesi için bir damar içi (IV) şant hazırlanması ve . Ven tuzlu enjekte açık olduğundan emin olun. Not: FITC dekstranın bir IV uygulama (yüksek molekül ağırlığı, 100 ul 10 mg / ml, 2,000 kDa) FCFM femoral mikrovaskuleterini görselleştirme kolaylaştırır. Doksorubisin (100 ul, 8 mg / kg, Adriyamisin) veya tuzlu su, aynı zamanda, daha sonra önceden ısıtılmış bir kuyruk venine IV tatbik edilecektir. </li><li> Bir polistiren sahnede supinely fareyi yerleştirin. Pad fare Güvenli ve cerrahi koli bandı kullanarak konumunu korumak. </li></ol>sırasında ve Doksorubisin veya Salin İdaresi sonra FCFM tarafından Femoral Damar başlığı &quot;&gt; 3. Görüntüleme NOT: (1) mikroprob (mini0 / 30): Bu çalışmada kullanılan lifli konfokal mikroskop iki üniteden oluşmaktadır. (2) Lazer tarama ünitesi (LSU-488; 488 nm dalga boyu). <ol><li> LSU 488 nm dalga boyunda lazer kullanarak analizler her zaman tur gerçekleştirin. NOT: Ana ünite dedektörü, filtre tespit (500-650 nm) yayılan floresan. edinilen görüntüler daha sonra yeniden ve / sn 12 kare hızında görüntülenir. </li><li> Dikkatle iğneden şırınga ayırın ve FITC dekstran içeren yeni bir şırınga takın. FITC dekstran (IV) 100 ul yönetme. </li><li> Karşılık gelen bir görüntü elde etmek için z ekseni uyum sonra, bakış uygun bir alanına mikroprob (mini0 / 30), Shift ve sabitleştirmek. Açık ve odaklanmış sinyal visi- kadar solmaya ilk sinyal bekleyinble. </li><li> Kısa bir süre stabilizasyon (~ 30 sn) için bir temel kan akışını kaydedin. Sonra doksorubisin veya tuzlu ya içeren, iğne başka şırınga bağlayın. IV, 100 ul doksorubisin veya tuzlu yönetmek. </li><li> 20 dakika boyunca sürekli olarak enjekte FITC dekstran akışını izleyin. FCFM ilişkili yazılım üzerinde, kan damarlarını ölçmek için üst cetvel üzerinde çapı düğmesini kullanın ve kategorize onları küçük (&lt;15 mikron) veya büyük (&gt; 15 mm). </li><li> Anestezi doz aşımı ile hayvan Euthanize. </li></ol>

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Kemoterapi ile indüklenen vasküler toksisitesinin değerlendirilmesi nedeniyle, gerçek zamanlı olarak bir uyarıcıya tepki olarak damar dinamiklerinin görüntülenmesi güçlüğü nedeniyle zordur. Birçok klinik çalışmalar birkaç kemoterapiler doğrudan vasküler yaralanmaya neden olduğunu ima var, ama bu toksisite mekanizması ve özellikleri tam olarak açıklığa kavuşmuş. Bu tarifnamede, 8-10 tarif edildiği gibi lifli konfokal floresan mikroskobu içeren farelerde kemoterapi potansiyel va...

Klinik kanıtlar kemoterapi çeşitli sınıfları Raynaud fenomeni, hipertansiyon, myocardialinfarction, serebrovasküler atak ve hepatik veno-occlusivedisease 1,2 ile kendini gösteren vasküler patolojilerin çeşitli ortaya belirtir. Başlangıçta bu amaçla 3-5 için geliştirilmiş, ancak az uygulanmasıyla tümör hücrelerini yok etmek için tasarlanmış olup, ancak &quot;&#39;kazara anti-anjiyojenik ilaçlar&quot;, &quot;yan mümkün anjiyojenez inhibitörleri olarak etki geleneksel kemoterapötik maddeleri tarif oldukça yeni bir terimdir mümkün 3 gibi normal hücrelere zarar &quot;. Serum biyomarkırları kullanarak klinik çalışmalarda görüldüğü gibi çeşitli kemoterapiler vasculo-toksik maddeler olarak ima edilmiştir. Bunlar arasında maddeler (örneğin, siklofosfamid gibi), platin (örneğin sisplatin gibi) bileşikleri ve antrasiklinler 1,2,5-7 alkile edilir. Akut kardiyovasküler komplikasyonlar Bir resul olarak ortaya çıkabilirKemoterapi ile uyarılan vasküler toksisite t. Onlar geç vasküler morbidite riski ateroskleroz ve hesabına gibi kronik koşulları dikkate ilerleyebilir. Ancak, klinik kanıt montaj rağmen, damar toksisite mekanizması vurgulayan belirlenen çalışmalar yetersizdir olduğunu ve bu nedenle, onlar indirebilmek tam patogenezi daha aydınlatılması garanti edilir. Kemoterapi kaynaklı vasküler toksisite mekanizması açığa büyük bir meydan okuma in vivo vasküler fonksiyonu soruşturma karmaşıklığı kaynaklanmaktadır. Biz burada kan akışını ve gemilerin özelliklerini yakalamak sağlayan farelerde in vivo moleküler görüntüleme yüksek çözünürlüklü bir platform açıklar. Bu platform, doğrudan tedaviye bağlı vasküler etkilerinin tespitini kolaylaştırır: gerçek zamanlı olarak, hem de aynı bireylerin içinde bir süre boyunca onları takip gibi.

<table><tbody><tr><td>general anesthesia</td><td>Fort Dodge Animal Health, IA, USA and Biove Laboratories, France</td><td></td><td>100 mg/kg ketaset and 6 mg/kg XYL-M2</td></tr><tr><td>depilatory cream (Veet)</td><td>ReckittBenckiser, Bristol, UK</td><td></td><td></td></tr><tr><td>30 G, 1/2 inch needle attached to 1 ml syringe</td><td></td><td></td><td></td></tr><tr><td>FITC dextran (10 mg/ml; MW 2,000 kDa)</td><td>Sigma</td><td>FD2000S</td><td>100 &amp;mu;l volume</td></tr><tr><td>Doxorubicin</td><td>Teva, Israel</td><td></td><td>8 mg/kg, Adriamycin</td></tr><tr><td>paclitaxel</td><td>Taro, Israel</td><td></td><td>1.2 mg/kg, Medexel</td></tr><tr><td>saline</td><td></td><td></td><td></td></tr></tbody></table>

chemotherapy-induced vascular toxicity - real-time in vivo imaging of vessel impairment

Kemoterapiler bazı sınıfları vasküler morbidite riski hastayı zemin hazırlayabilir uzun vadeli koşulları içine ilerleyebilir akut vasküler değişiklikleri uygulamak olabilir. Ancak, montaj klinik kanıt olsa, vasküler toksisite ve bu nedenle damar / kardiyovasküler bozukluklar heterojen bir grup etiyolojisinde açık çalışmalar yetersizdir aydınlatılamamıştır gerekmektedir yoktur. Ayrıca, damar toksisite yatan mekanizma olabilir tamamen miyosit yaralanma doğrudan ilgilidir kemoterapi kaynaklı kardiyotoksisitenin, ilkelerinden farklı olabilir. Biz anti-kanser tedavilerinin olası akut vasküler toksisite değerlendirmek için in vivo moleküler görüntüleme platformu, gerçek zamanlı kurduk. Biz uygun kapalı organ ve referans blo içinde damarsal görselleştirmek için in vivo, farelerde yüksek çözünürlüklü moleküler görüntüleme, bir platformu kurdunuzHer birey ise aynı bireyler içinde od gemiler kendi kontrolü olarak hizmet vermektedir. Kan damarı duvarları uç organ hasarı erken bir olay olabilir vasküler toksisite benzersiz bir mekanizma temsil, doksorubisin uygulamadan sonra bozulmaktadır. Burada, lifli konfokal floresan mikroskobu (FCFM) göre görüntüleme yöntemi, hayvan deneklerde hücresel ve alt-hücresel düzeyde fizyolojik olayları anlamak için yenilikçi bir mod sağlayan, tarif edilmektedir.

Gerçek zamanlı olarak in vivo sürekli görüntülemede Burada kullanılan görüntüleme aparatı, damar görselleştirme ve kemoterapi gibi çeşitli uyarıcılara verdiği cevap sağlayan bir sonda ile donatılmış bir yüksek çözünürlüklü, lifli konfokal mikroskop vardır. Derin damarlar ya da organ görüntüleme kolaylaştırabilir rağmen, sonda için küçük bir insizyon, bu yöntem minimal invaziv. prob demetleri onlarca lifleri, mikroskop optik ve hassas ö...

Watch this Scientific Journal Video about Chemotherapy-induced Vascular Toxicity - Real-time In vivo Imaging of Vessel Impairment at JoVE.com

Chemotherapy-induced Vascular Toxicity - Real-time In vivo Imaging of Vessel Impairment

We herein describe the method of fibered confocal fluorescent microscopy (FCFM) based imaging, which provides an innovative mode to understand physiological phenomena at the cellular and sub-cellular levels in animal subjects.

Kemoterapi kaynaklı Vasküler Toksisite - Gerçek zamanlı<em&gt; In vivo</em&gt; Gemi Düşüklüğü Görüntüleme

Certain classes of chemotherapies may exert acute vascular changes that may progress into long-term conditions that may predispose the patient to an ...

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Chemotherapy-induced Vascular Toxicity - Real-time In vivo Imaging of Vessel Impairment

7.3K Görüntüleme.  Tel Aviv University. We herein describe the method of fibered confocal fluorescent microscopy (FCFM) based imaging, which provides an innovative mode to understand physiological phenomena at the cellular and sub-cellular levels in animal subjects.

Video: Chemotherapy-induced Vascular Toxicity - Real-time In vivo Imaging of Vessel Impairment

Contrast Enhanced Vessel Imaging using MicroCT

Microscopic computed tomography (microCT) offers high-resolution volumetric imaging of the anatomy of living small animals. However, the contrast between different soft tissues and body fluids is inherently poor in micro-CT images 1. Under these circumstances, visualization of blood vessels becomes a nearly impossible task. To overcome this and to improve the visualization of blood vessels exogenous contrast agents can be used. Herein, we present a methodology for visualizing the vascular network in a rodent model. By using a long-acting aqueous colloidal polydisperse iodinated blood-pool contrast agent, eXIA 160XL, we optimized image acquisition parameters and volume-rendering techniques for finding blood vessels in live animals. Our findings suggest that, to achieve a superior contrast between bone and soft tissue from vessel, multiple-frames (at least 5-8/ frames per view), and 360-720 views (for a full 360&deg; rotation) acquisitions were mandatory. We have also demonstrated the use of a two-dimensional transfer function (where voxel color and opacity was assigned in proportion to CT value and gradient magnitude), in visualizing the anatomy and highlighting the structure of interest, the blood vessel network. This promising work lays a foundation for the qualitative and quantitative assessment of anti-angiogenesis preclinical studies using transgenic or xenograft tumor-bearing mice.

Contrast enhanced small animal vessel imaging by microCT is a rapid, cost-effective and high-throughput technique for serial in situ examination for tumor development, for analyzing the network of blood vessels that nourish them, and for following the response of tumors to preclinical therapeutic intervention(s).

Kontrast Geliştirilmiş Gemi Görüntüleme MicroCT kullanarak

Microscopic computed tomography (microCT) offers high-resolution volumetric imaging of the anatomy of living small animals. However, the contrast between ...

Tıp

Live Imaging of Drug Responses in the Tumor Microenvironment in Mouse Models of Breast Cancer

The tumor microenvironment plays a pivotal role in tumor initiation, progression, metastasis, and the response to anti-cancer therapies. Three-dimensional co-culture systems are frequently used to explicate tumor-stroma interactions, including their role in drug responses. However, many of the interactions that occur in vivo in the intact microenvironment cannot be completely replicated in these in vitro settings. Thus, direct visualization of these processes in real-time has become an important tool in understanding tumor responses to therapies and identifying the interactions between cancer cells and the stroma that can influence these responses. Here we provide a method for using spinning disk confocal microscopy of live, anesthetized mice to directly observe drug distribution, cancer cell responses and changes in tumor-stroma interactions following administration of systemic therapy in breast cancer models. We describe procedures for labeling different tumor components, treatment of animals for observing therapeutic responses, and the surgical procedure for exposing tumor tissues for imaging up to 40 hours. The results obtained from this protocol are time-lapse movies, in which such processes as drug infiltration, cancer cell death and stromal cell migration can be evaluated using image analysis software.

We describe a method for imaging response to anti-cancer treatment in vivo and at single cell resolution.

Meme Kanseri Fare Modelleri Tümör Mikroçevre İlaç Yanıtlarının Görüntüleme Canlı

The tumor microenvironment plays a pivotal role in tumor initiation, progression, metastasis, and the response to anti-cancer therapies. Three-dimensional ...

Real-time Imaging of Heterotypic Platelet-neutrophil Interactions on the Activated Endothelium During Vascular Inflammation and Thrombus Formation in Live Mice

Interaction of activated platelets and leukocytes (mainly neutrophils) on the activated endothelium mediates thrombosis and vascular inflammation.1,2 During thrombus formation at the site of arteriolar injury, platelets adherent to the activated endothelium and subendothelial matrix proteins support neutrophil rolling and adhesion.3 Conversely, under venular inflammatory conditions, neutrophils adherent to the activated endothelium can support adhesion and accumulation of circulating platelets. Heterotypic platelet-neutrophil aggregation requires sequential processes by the specific receptor-counter receptor interactions between cells.4 It is known that activated endothelial cells release adhesion molecules such as von Willebrand factor, thereby initiating platelet adhesion and accumulation under high shear conditions.5 Also, activated endothelial cells support neutrophil rolling and adhesion by expressing selectins and intercellular adhesion molecule-1 (ICAM-1), respectively, under low shear conditions.4 Platelet P-selectin interacts with neutrophils through P-selectin glycoprotein ligand-1 (PSGL-1), thereby inducing activation of neutrophil &beta;2 integrins and firm adhesion between two cell types. Despite the advances in in vitro experiments in which heterotypic platelet-neutrophil interactions are determined in whole blood or isolated cells,6,7 those studies cannot manipulate oxidant stress conditions during vascular disease. In this report, using fluorescently-labeled, specific antibodies against a mouse platelet and neutrophil marker, we describe a detailed intravital microscopic protocol to monitor heterotypic interactions of platelets and neutrophils on the activated endothelium during TNF-&alpha;-induced inflammation or following laser-induced injury in cremaster muscle microvessels of live mice.

Here we report an experimental technique of fluorescence intravital microscopy to visualize heterotypic platelet-neutrophil interactions on the activated endothelium during vascular inflammation and thrombus formation in live mice. This microscopic technology will be valuable to study the molecular mechanism of vascular disease and to test pharmacologic agents under pathophysiological conditions.

Canlı Fare Damar İltihabı ve trombüs oluşumu sırasında Activated Endotel üzerinde heterotypic Trombosit nötrofil Etkileşimleri Gerçek zamanlı Görüntüleme

Interaction of activated platelets and leukocytes (mainly neutrophils) on the activated endothelium mediates thrombosis and vascular inflammation.1,2 ...

İmmünoloji ve Enfeksiyon

In vivo Imaging of Tumor Angiogenesis using Fluorescence Confocal Videomicroscopy

Fibered confocal fluorescence in vivo imaging with a fiber optic bundle uses the same principle as fluorescent confocal microscopy. It can excite fluorescent in situ elements through the optical fibers, and then record some of the emitted photons, via the same optical fibers. The light source is a laser that sends the exciting light through an element within the fiber bundle and as it scans over the sample, recreates an image pixel by pixel. As this scan is very fast, by combining it with dedicated image processing software, images in real time with a frequency of 12 frames/sec can be obtained.
We developed a technique to quantitatively characterize capillary morphology and function, using a confocal fluorescence videomicroscopy device. The first step in our experiment was to record 5 sec movies in the four quadrants of the tumor to visualize the capillary network. All movies were processed using software (ImageCell, Mauna Kea Technology, Paris France) that performs an automated segmentation of vessels around a chosen diameter (10 &mu;m in our case). Thus, we could quantify the 'functional capillary density', which is the ratio between the total vessel area and the total area of the image. This parameter was a surrogate marker for microvascular density, usually measured using pathology tools.
The second step was to record movies of the tumor over 20 min to quantify leakage of the macromolecular contrast agent through the capillary wall into the interstitium. By measuring the ratio of signal intensity in the interstitium over that in the vessels, an 'index leakage' was obtained, acting as a surrogate marker for capillary permeability.

In vivo Imaging of Tumor Angiogenesis using Fluorescence Confocal Videomicroscopy

In this paper, we present a method to analyze tumor microvessels in vivo using dynamic contrast-enhanced fluorescence videomicroscopy. Two quantitative parameters were acquired: functional capillary density reflecting the vascularity of the tumor, and index leakage reflecting the leakiness of the endothelial wall.

Flüoresans Konfokal videomikroskopi kullanılarak tümör Angiogenesis İn vivo

Fibered confocal fluorescence in vivo imaging with a fiber optic bundle uses the same principle as fluorescent confocal microscopy. It can excite ...

A Novel High-resolution In vivo Imaging Technique to Study the Dynamic Response of Intracranial Structures to Tumor Growth and Therapeutics

We have successfully integrated previously established Intracranial window (ICW) technology 1-4 with intravital 2-photon confocal microscopy to develop a novel platform that allows for direct long-term visualization of tissue structure changes intracranially. Imaging at a single cell resolution in a real-time fashion provides supplementary dynamic information beyond that provided by standard end-point histological analysis, which looks solely at 'snap-shot' cross sections of tissue.
Establishing this intravital imaging technique in fluorescent chimeric mice, we are able to image four fluorescent channels simultaneously. By incorporating fluorescently labeled cells, such as GFP+ bone marrow, it is possible to track the fate of these cells studying their long-term migration, integration and differentiation within tissue. Further integration of a secondary reporter cell, such as an mCherry glioma tumor line, allows for characterization of cell:cell interactions. Structural changes in the tissue microenvironment can be highlighted through the addition of intra-vital dyes and antibodies, for example CD31 tagged antibodies and Dextran molecules.
Moreover, we describe the combination of our ICW imaging model with a small animal micro-irradiator that provides stereotactic irradiation, creating a platform through which the dynamic tissue changes that occur following the administration of ionizing irradiation can be assessed.
Current limitations of our model include penetrance of the microscope, which is limited to a depth of up to 900 &mu;m from the sub cortical surface, limiting imaging to the dorsal axis of the brain. The presence of the skull bone makes the ICW a more challenging technical procedure, compared to the more established and utilized chamber models currently used to study mammary tissue and fat pads 5-7. In addition, the ICW provides many challenges when optimizing the imaging.

A Novel High-resolution In vivo Imaging Technique to Study the Dynamic Response of Intracranial Structures to Tumor Growth and Therapeutics

We describe a novel in vivo imaging technique that couples fluorescent chimeric mice with intracranial windows and high-resolution 2-photon microscopy. This imaging platform aids studies of dynamic changes in brain tissue and microvasculature, at a single-cell level, following pathological insults and is adaptable to assess intracranial drug delivery and distribution.

Yeni Bir Yüksek çözünürlüklü<em&gt; In vivo</emTümör Büyüme ve Tedavi için İntrakranial Yapıların Dinamik Tepki Eğitim&gt; Görüntüleme Tekniği

We have successfully integrated previously established Intracranial window (ICW) technology 1-4 with intravital 2-photon confocal microscopy to develop a ...

Mikroakışkan Tabanlı Vaskülarize İnsan Mikro Tümör Modelinin Hazırlanması

Establishing a Physiologic Human Vascularized Micro-Tumor Model for Cancer Research

A lack of validated cancer models that recapitulate the tumor microenvironment of solid cancers in vitro remains a significant bottleneck for preclinical cancer research and therapeutic development.&#160;To overcome this problem, we have developed the vascularized microtumor (VMT), or tumor chip, a microphysiological system that realistically models the complex human tumor microenvironment. The VMT forms de novo within a microfluidic platform by co-culture of multiple human cell types under dynamic, physiological flow conditions. This tissue-engineered micro-tumor construct incorporates a living perfused vascular network that supports the growing tumor mass just as newly formed vessels do in vivo. Importantly, drugs and immune cells must cross the endothelial layer to reach the tumor, modeling in vivo physiological barriers to therapeutic delivery and efficacy. Since the VMT platform is optically transparent, high-resolution imaging of dynamic processes such as immune cell extravasation and metastasis can be achieved with direct visualization of fluorescently labeled cells within the tissue. Further, the VMT retains in vivo tumor heterogeneity, gene expression signatures, and drug responses. Virtually any tumor type can be adapted to the platform, and primary cells from fresh surgical tissues grow and respond to drug treatment in the VMT, paving the way toward truly personalized medicine. Here, the methods for establishing the VMT and utilizing it for oncology research are outlined. This innovative approach opens new possibilities for studying tumors and drug responses, providing researchers with a powerful tool to advance cancer research.

Yazar Spotlight: Translasyonel Kanser Araştırmaları için Model Olarak İnsan Vaskülarize Mikro Tümörleri Oluşturma 

This protocol presents a physiologically relevant tumor-on-a-chip model to perform high-throughput basic and translational human cancer research, advancing drug screening, disease modeling, and personalized medicine approaches with a description of loading, maintenance, and evaluation procedures.

Kanser Araştırmaları için Fizyolojik İnsan Vaskülarize Mikro-Tümör Modelinin Oluşturulması

A lack of validated cancer models that recapitulate the tumor microenvironment of solid cancers in vitro remains a significant bottleneck for preclinical ...

Kanser Araştırmaları

Farelerde Rehberli Tümör Aşılaması ve Multimodal Görüntüleme

A Dorsal Skinfold Window Chamber Tumor Mouse Model for Combined Intravital Microscopy and Magnetic Resonance Imaging in Translational Cancer Research

Preclinical intravital imaging such as microscopy and optical coherence tomography have proven to be valuable tools in cancer research for visualizing the tumor microenvironment and its response to therapy. These imaging modalities have micron-scale resolution but have limited use in the clinic due to their shallow penetration depth into tissue. More clinically applicable imaging modalities such as CT, MRI, and PET have much greater penetration depth but have comparatively lower spatial resolution (mm scale). 
To translate preclinical intravital imaging findings into the clinic, new methods must be developed to bridge this micro-to-macro resolution gap. Here we describe a dorsal skinfold window chamber tumor mouse model designed to enable preclinical intravital and clinically applicable (CT and MR) imaging in the same animal, and the image analysis platform that links these two disparate visualization methods. Importantly, the described window chamber approach enables the different imaging modalities to be co-registered in 3D using fiducial markers on the window chamber for direct spatial concordance. This model can be used for validation of existing clinical imaging methods, as well as for the development of new ones through direct correlation with "ground truth" high-resolution intravital findings. 
Finally, the tumor response to various treatments-chemotherapy, radiotherapy, photodynamic therapy-can be monitored longitudinally with this methodology using preclinical and clinically applicable imaging modalities. The dorsal skinfold window chamber tumor mouse model and imaging platforms described here can thus be used in a variety of cancer research studies, for example, in translating preclinical intravital microscopy findings to more clinically applicable imaging modalities such as CT or MRI.

Yazar Spotlight: Stereotaktik Vücut Radyasyon Tedavisi Planlamasını Geliştirmek için Yüksek Çözünürlüklü İntravital Görüntüleme ve MRG'yi Entegre Etme

Translation of Intravital microscopy findings is challenged by its shallow depth penetration into tissue. Here we describe a dorsal window chamber mouse model that enables co-registration of intravital microscopy and clinically applicable imaging modalities (e.g., CT, MRI) for direct spatial correlation, potentially streamlining clinical translation of intravital microscopy findings.

Translasyonel Kanser Araştırmalarında Kombine İntravital Mikroskopi ve Manyetik Rezonans Görüntüleme için Dorsal Deri Kıvrımlı Pencere Odası Tümör Fare Modeli

Preclinical intravital imaging such as microscopy and optical coherence tomography have proven to be valuable tools in cancer research for visualizing the ...

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Kanser Araştırmaları için Fizyolojik İnsan Vaskülarize Mikro-Tümör Modelinin Oluşturulması

Kanser Araştırmaları için Fizyolojik İnsan Vaskülarize Mikro-Tümör Modelinin Oluşturulması

Kanser Araştırmaları için Fizyolojik İnsan Vaskülarize Mikro-Tümör Modelinin Oluşturulması

Translasyonel Kanser Araştırmalarında Kombine İntravital Mikroskopi ve Manyetik Rezonans Görüntüleme için Dorsal Deri Kıvrımlı Pencere Odası Tümör Fare Modeli

Translasyonel Kanser Araştırmalarında Kombine İntravital Mikroskopi ve Manyetik Rezonans Görüntüleme için Dorsal Deri Kıvrımlı Pencere Odası Tümör Fare Modeli