윤리 문 : 모든 실험 기관 동물 관리 및 사용위원회에 의해 승인되었습니다. 동물 보호는 제도적 지침에 따라이었다. (- 8 주 오래 된 7; 25-30g) ICR 여성 마우스는 텔 아비브 대학에서 의학 학부 Sackler의 에어컨, 조명 제어 동물 시설에 수용되었다. 용어에서, 동물은 마취 과다 복용으로 안락사시켰다. 1. 섬유질의 공 초점 형광 현미경 (FCFM) 교정 <ol><li> 장치를 켜십시오. </li><li> 마이크로 탐침 (mini0이 / 30)을 연결합니다. </li><li> 제조업체의 지시에 따라 교정 장치. </li></ol> 2. 마우스 이미징을위한 준비 <ol><li> 모두 Ketaset (100 ㎎ / ㎏)과 XYL-M2 (6 ㎎ / ㎏)의 피하 주사에 의해 마취. 발가락 핀치에 응답하지 않는 문제로 적절한 마취를 확인합니다. </li><li> 순서에 사타구니 아래 피부를 절개<html> 대퇴 동맥 혈관을 알 수있다. 절개 다음 식염수 촉촉한 절개 사이트를 유지합니다. </li><li> 약 30 초 동안 따뜻한 물 (너무 뜨겁지 않은 터치)로 가득 찬 가방 (또는 장갑)를 사용하여 꼬리를 가열. 식염수 또는 화학 요법 제 중 하나의 꼬리 정맥에 주사 바늘 (30 G, 1/2 인치)를 삽입하고 여기에 1 ML의 주사기를 부착하여 FITC 덱스 트란 (조영제)의 관리를위한 정맥 주사 (IV) 션트를 준비하고 . 정맥은 식염수를 주입하여 열려 있는지 확인하십시오. 참고 : FITC 덱스 트란의 IV 관리 (고 분자량 100 ㎕의 10 ㎎을 / ㎖, 2000 kDa의)이 FCFM에 의한 대퇴 미세 혈관을 시각화 용이. 독소루비신 (100 μL; 8 ㎎ / kg, 아드리아 마이신) 또는 염수 나중에 예열 꼬리 정맥으로 IV 투여 될 것이다. </li><li> 폴리스티렌 무대에 드러 마우스를 놓습니다. 패드에 마우스를 안전하고 수술 덕트 테이프를 사용하여 위치를 유지한다. </li></ol>동안과 독소루비신 또는 식염수를 투여 한 후 FCFM에 의한 대퇴 혈관의 제목 &quot;&gt; 3. 영상 참고 : (1) 마이크로 탐침 (mini0이 / 30) :이 연구에 사용 된 다발, 공 초점 현미경은 두 개의 유닛으로 구성되어있다. (2) 레이저 스캐닝 유닛 (LSU-488; 488 nm 파장). <ol><li> LSU 488 nm 파장의 레이저를 이용하여 분석 모든 시간 바퀴를 수행합니다. 참고 : 본체 검출기는 여과 감지 (500-650 nm의) 방출 형광을. 획득 된 이미지는 이후 재구성 / 초 12 프레임의 속도로 표시됩니다. </li><li> 조심스럽게 바늘의 주사기를 분리하고 FITC 덱스 트란을 포함하는 새로운 주사기를 연결합니다. FITC 덱스 트란의 (IV) 100 μl를 관리합니다. </li><li> 대응하는 화상을 얻기 위해서, Z 축 조정 다음에, 뷰 필드에 적합한 마이크로 프로브 (mini0 / 30)를 시프트하고 흥분시키는. 명확하고 초점을 맞춘 신호가 VISI 때까지 페이드 초기 신호를 기다립니다BLE. </li><li> 짧은 안정화 기간 (~ 30 초)에 대한 기준선 혈액의 흐름을 기록합니다. 그런 다음 독소루비신 또는 식염수를 포함, 바늘에 다른 주사기를 연결합니다. IV 100 μL 독소루비신 또는 식염수를 관리 할 수​​ 있습니다. </li><li> 20 분 동안 연속적으로 주입 FITC 덱스 트란의 흐름을 모니터링한다. FCFM 관련된 소프트웨어를, 혈관을 측정하기 위해 상부에 눈금자 직경 버튼을 사용하여 분류하고 그들 작은 (&lt;15㎛ 인) 또는 큰 (&gt; ~ 15㎛). </li><li> 마취 과다 복용으로 동물을 안락사. </li></ol>

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화학 요법 - 유도 된 혈관 독성을 평가하기 때문에 실시간으로 자극에 반응하여 혈관의 역학을 시각화하는 어려움에 도전. 수많은 임상 연구는 여러 가지 화학 요법 직접 혈관 손상을 일으킬 것으로 연루, 아직이 독성의 메커니즘과 특성은 밝혀지지 남아있다. 우리는 여기에 8 ~ 10 설명 된 바와 같이 다발, 공 초점 형광 현미경으로 구성된 마우스에서 화학 요법의 잠재적 인 혈관 독성을 평...

임상 증거는 화학 요법의 여러 클래스가 레이노 현상, 고혈압, myocardialinfarction, 뇌 혈관 공격, 간성 veno-occlusivedisease 1, 2에 의해 나타난 혈관 병변의 다양성을 이끌어내는 것을 나타냅니다. 그들은 원래 이러한 목적 3-5으로 개발되지만 적게 부과함으로써 종양 세포를 제거하도록 설계되지는 않았지만 &quot; &#39;사고&#39;항 혈관 형성 약제&quot;는 &quot;담보 가능한 혈관 신생 억제제로서 작용 종래의 화학 요법 제에 대해 설명 신종 용어이며 가능한 3과 정상 세포의 손상 &quot;. 혈청 바이오 마커를 사용하여 임상 연구에서 관찰 된 여러 화학 요법은 vasculo - 독성 물질로 암시되고있다. 이 중 에이전트 (예 : 시클로 포스 파 미드 등), 백금 (시스플라틴 등) 화합물 및 안트라 사이클린 1,2,5-7 알킬화된다. 급성 심장 혈관 합병증은 resul로 발생할 수 있습니다화학 요법에 의해 유도 된 혈관 독성 t. 그들은 늦게 혈관 사망률의 위험이 증가 동맥 경화 및 계정 등의 만성 질환으로 발전 할 수 있습니다. 그러나 임상 적 증거를 장착에도 불구하고, 혈관 독성의 메카니즘을 강조 지정된 연구가 소수이므로, 그들은 입힐 정확한 병인의 추가 해명이 보증된다. 화학 요법에 의한 혈관 독성의 메커니즘을 밝히는데 중요한 과제는 생체 내에서 혈관 기능을 조사의 복잡성에서 유래. 우리는 본원에서 혈류 및 혈관 &#39;특성을 포착 할 수 있도록 마우스에서 생체 분자 이미징에 대한 고해상도의 플랫폼을 설명한다. 이 플랫폼은 직접 유도 혈관 치료 효과의 검출을 용이하게한다 : 실시간뿐만 동일인 내의 일정 기간 동안 이들을 다음과 같이.

<table><tbody><tr><td>general anesthesia</td><td>Fort Dodge Animal Health, IA, USA and Biove Laboratories, France</td><td></td><td>100 mg/kg ketaset and 6 mg/kg XYL-M2</td></tr><tr><td>depilatory cream (Veet)</td><td>ReckittBenckiser, Bristol, UK</td><td></td><td></td></tr><tr><td>30 G, 1/2 inch needle attached to 1 ml syringe</td><td></td><td></td><td></td></tr><tr><td>FITC dextran (10 mg/ml; MW 2,000 kDa)</td><td>Sigma</td><td>FD2000S</td><td>100 &amp;mu;l volume</td></tr><tr><td>Doxorubicin</td><td>Teva, Israel</td><td></td><td>8 mg/kg, Adriamycin</td></tr><tr><td>paclitaxel</td><td>Taro, Israel</td><td></td><td>1.2 mg/kg, Medexel</td></tr><tr><td>saline</td><td></td><td></td><td></td></tr></tbody></table>

chemotherapy-induced vascular toxicity - real-time in vivo imaging of vessel impairment

화학 요법은 특정 부류의 병적 혈관의 증가 된 위험에 걸리기 쉬운 환자 수 장기적인 상태로 진행​​할 수있다 급성 혈관 변화를 가할 수있다. 그러나, 장착 임상 증거에도 불구하고, 혈관 독성 때문에 혈관 / 심혈관 질환의 이종 그룹의 원인의 명확한 연구의 소수는 밝혀지지 남아있다. 또한, 혈관 독성의 기초가있다 메커니즘은 완전히 심근 손상을 유도하는 관련된 화학 요법 - 유도 심독성의 원리와 다를 수있다. 우리는 항암 치료의 잠재적 급성 혈관 독성을 평가하는 생체 분자 이미징 플랫폼에서 실시간을 확립했다. 우리는 적절한 밀폐 된 기관 및 참조 BLO 내 혈관을 시각화 생체, 마우스의 고해상도 분자 영상의 플랫폼을 설정 한각 개별 반면 같은 개인 내 외경 선박은 자체 제어 역할을합니다. 혈관벽 최종 장기 손상을 조기 이벤트가 될 수있다 혈관 독성을 나타내는 고유기구, 독소루비신 투여 후 손상되었다. 여기서, 다발, 공 초점 형광 현미경 (FCFM) 기반 이미징의 방법은 동물 피험자에서 세포 및 세포 이하 수준에서의 생리 현상을 이해하기 위해 혁신적인 모드를 제공하는 설명한다.

실시간 생체 내 이미징 연속 여기에 사용되는 촬상 장치는, 혈관계의 시각화 및 화학 요법과 같은 다양한 자극에 대한 응답을 가능하게 장착 된 프로브 고화질, 다발, 공 초점 현미경이다. 깊숙히 혈관 또는 기관의 영상을 용이하게 할 수 있지만,이 프로브 작은 절개를 필요로하기 때문에이 방법은 침습적이다. 프로브 번들은 수십 섬유, 현미경 광학 및 독점 정밀 커넥터?...

Watch this Scientific Journal Video about Chemotherapy-induced Vascular Toxicity - Real-time In vivo Imaging of Vessel Impairment at JoVE.com

Chemotherapy-induced Vascular Toxicity - Real-time In vivo Imaging of Vessel Impairment

We herein describe the method of fibered confocal fluorescent microscopy (FCFM) based imaging, which provides an innovative mode to understand physiological phenomena at the cellular and sub-cellular levels in animal subjects.

화학 요법에 의한 혈관 독성 - 실시간<em&gt; 생체 내</em&gt; 선박 손상의 영상

Certain classes of chemotherapies may exert acute vascular changes that may progress into long-term conditions that may predispose the patient to an ...

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Chemotherapy-induced Vascular Toxicity - Real-time In vivo Imaging of Vessel Impairment

7.4K 조회수.  Tel Aviv University. We herein describe the method of fibered confocal fluorescent microscopy (FCFM) based imaging, which provides an innovative mode to understand physiological phenomena at the cellular and sub-cellular levels in animal subjects.

비디오: Chemotherapy-induced Vascular Toxicity - Real-time In vivo Imaging of Vessel Impairment

Contrast Enhanced Vessel Imaging using MicroCT

Microscopic computed tomography (microCT) offers high-resolution volumetric imaging of the anatomy of living small animals. However, the contrast between different soft tissues and body fluids is inherently poor in micro-CT images 1. Under these circumstances, visualization of blood vessels becomes a nearly impossible task. To overcome this and to improve the visualization of blood vessels exogenous contrast agents can be used. Herein, we present a methodology for visualizing the vascular network in a rodent model. By using a long-acting aqueous colloidal polydisperse iodinated blood-pool contrast agent, eXIA 160XL, we optimized image acquisition parameters and volume-rendering techniques for finding blood vessels in live animals. Our findings suggest that, to achieve a superior contrast between bone and soft tissue from vessel, multiple-frames (at least 5-8/ frames per view), and 360-720 views (for a full 360&deg; rotation) acquisitions were mandatory. We have also demonstrated the use of a two-dimensional transfer function (where voxel color and opacity was assigned in proportion to CT value and gradient magnitude), in visualizing the anatomy and highlighting the structure of interest, the blood vessel network. This promising work lays a foundation for the qualitative and quantitative assessment of anti-angiogenesis preclinical studies using transgenic or xenograft tumor-bearing mice.

Contrast enhanced small animal vessel imaging by microCT is a rapid, cost-effective and high-throughput technique for serial in situ examination for tumor development, for analyzing the network of blood vessels that nourish them, and for following the response of tumors to preclinical therapeutic intervention(s).

MicroCT를 사용하여 대비 향상된 베셀 영상

Microscopic computed tomography (microCT) offers high-resolution volumetric imaging of the anatomy of living small animals. However, the contrast between ...

연구

의학

Live Imaging of Drug Responses in the Tumor Microenvironment in Mouse Models of Breast Cancer

The tumor microenvironment plays a pivotal role in tumor initiation, progression, metastasis, and the response to anti-cancer therapies. Three-dimensional co-culture systems are frequently used to explicate tumor-stroma interactions, including their role in drug responses. However, many of the interactions that occur in vivo in the intact microenvironment cannot be completely replicated in these in vitro settings. Thus, direct visualization of these processes in real-time has become an important tool in understanding tumor responses to therapies and identifying the interactions between cancer cells and the stroma that can influence these responses. Here we provide a method for using spinning disk confocal microscopy of live, anesthetized mice to directly observe drug distribution, cancer cell responses and changes in tumor-stroma interactions following administration of systemic therapy in breast cancer models. We describe procedures for labeling different tumor components, treatment of animals for observing therapeutic responses, and the surgical procedure for exposing tumor tissues for imaging up to 40 hours. The results obtained from this protocol are time-lapse movies, in which such processes as drug infiltration, cancer cell death and stromal cell migration can be evaluated using image analysis software.

We describe a method for imaging response to anti-cancer treatment in vivo and at single cell resolution.

유방암의 마우스 모델에서 종양 Microenvironment에 약물 응답의 영상을 라​​이브

The tumor microenvironment plays a pivotal role in tumor initiation, progression, metastasis, and the response to anti-cancer therapies. Three-dimensional ...

Real-time Imaging of Heterotypic Platelet-neutrophil Interactions on the Activated Endothelium During Vascular Inflammation and Thrombus Formation in Live Mice

Interaction of activated platelets and leukocytes (mainly neutrophils) on the activated endothelium mediates thrombosis and vascular inflammation.1,2 During thrombus formation at the site of arteriolar injury, platelets adherent to the activated endothelium and subendothelial matrix proteins support neutrophil rolling and adhesion.3 Conversely, under venular inflammatory conditions, neutrophils adherent to the activated endothelium can support adhesion and accumulation of circulating platelets. Heterotypic platelet-neutrophil aggregation requires sequential processes by the specific receptor-counter receptor interactions between cells.4 It is known that activated endothelial cells release adhesion molecules such as von Willebrand factor, thereby initiating platelet adhesion and accumulation under high shear conditions.5 Also, activated endothelial cells support neutrophil rolling and adhesion by expressing selectins and intercellular adhesion molecule-1 (ICAM-1), respectively, under low shear conditions.4 Platelet P-selectin interacts with neutrophils through P-selectin glycoprotein ligand-1 (PSGL-1), thereby inducing activation of neutrophil &beta;2 integrins and firm adhesion between two cell types. Despite the advances in in vitro experiments in which heterotypic platelet-neutrophil interactions are determined in whole blood or isolated cells,6,7 those studies cannot manipulate oxidant stress conditions during vascular disease. In this report, using fluorescently-labeled, specific antibodies against a mouse platelet and neutrophil marker, we describe a detailed intravital microscopic protocol to monitor heterotypic interactions of platelets and neutrophils on the activated endothelium during TNF-&alpha;-induced inflammation or following laser-induced injury in cremaster muscle microvessels of live mice.

Here we report an experimental technique of fluorescence intravital microscopy to visualize heterotypic platelet-neutrophil interactions on the activated endothelium during vascular inflammation and thrombus formation in live mice. This microscopic technology will be valuable to study the molecular mechanism of vascular disease and to test pharmacologic agents under pathophysiological conditions.

라이브 마우스의 혈관 염증과 혈전 형성하는 동안 활성화 내피에 Heterotypic 혈소판 - 호중구 상호 작용의 실시간 영상

Interaction of activated platelets and leukocytes (mainly neutrophils) on the activated endothelium mediates thrombosis and vascular inflammation.1,2 ...

면역학 및 감염병학

In vivo Imaging of Tumor Angiogenesis using Fluorescence Confocal Videomicroscopy

Fibered confocal fluorescence in vivo imaging with a fiber optic bundle uses the same principle as fluorescent confocal microscopy. It can excite fluorescent in situ elements through the optical fibers, and then record some of the emitted photons, via the same optical fibers. The light source is a laser that sends the exciting light through an element within the fiber bundle and as it scans over the sample, recreates an image pixel by pixel. As this scan is very fast, by combining it with dedicated image processing software, images in real time with a frequency of 12 frames/sec can be obtained.
We developed a technique to quantitatively characterize capillary morphology and function, using a confocal fluorescence videomicroscopy device. The first step in our experiment was to record 5 sec movies in the four quadrants of the tumor to visualize the capillary network. All movies were processed using software (ImageCell, Mauna Kea Technology, Paris France) that performs an automated segmentation of vessels around a chosen diameter (10 &mu;m in our case). Thus, we could quantify the 'functional capillary density', which is the ratio between the total vessel area and the total area of the image. This parameter was a surrogate marker for microvascular density, usually measured using pathology tools.
The second step was to record movies of the tumor over 20 min to quantify leakage of the macromolecular contrast agent through the capillary wall into the interstitium. By measuring the ratio of signal intensity in the interstitium over that in the vessels, an 'index leakage' was obtained, acting as a surrogate marker for capillary permeability.

In vivo Imaging of Tumor Angiogenesis using Fluorescence Confocal Videomicroscopy

In this paper, we present a method to analyze tumor microvessels in vivo using dynamic contrast-enhanced fluorescence videomicroscopy. Two quantitative parameters were acquired: functional capillary density reflecting the vascularity of the tumor, and index leakage reflecting the leakiness of the endothelial wall.

형광 공 초점 Videomicroscopy를 사용하여 종양 혈관의 생체 내 이미징

Fibered confocal fluorescence in vivo imaging with a fiber optic bundle uses the same principle as fluorescent confocal microscopy. It can excite ...

A Novel High-resolution In vivo Imaging Technique to Study the Dynamic Response of Intracranial Structures to Tumor Growth and Therapeutics

We have successfully integrated previously established Intracranial window (ICW) technology 1-4 with intravital 2-photon confocal microscopy to develop a novel platform that allows for direct long-term visualization of tissue structure changes intracranially. Imaging at a single cell resolution in a real-time fashion provides supplementary dynamic information beyond that provided by standard end-point histological analysis, which looks solely at 'snap-shot' cross sections of tissue.
Establishing this intravital imaging technique in fluorescent chimeric mice, we are able to image four fluorescent channels simultaneously. By incorporating fluorescently labeled cells, such as GFP+ bone marrow, it is possible to track the fate of these cells studying their long-term migration, integration and differentiation within tissue. Further integration of a secondary reporter cell, such as an mCherry glioma tumor line, allows for characterization of cell:cell interactions. Structural changes in the tissue microenvironment can be highlighted through the addition of intra-vital dyes and antibodies, for example CD31 tagged antibodies and Dextran molecules.
Moreover, we describe the combination of our ICW imaging model with a small animal micro-irradiator that provides stereotactic irradiation, creating a platform through which the dynamic tissue changes that occur following the administration of ionizing irradiation can be assessed.
Current limitations of our model include penetrance of the microscope, which is limited to a depth of up to 900 &mu;m from the sub cortical surface, limiting imaging to the dorsal axis of the brain. The presence of the skull bone makes the ICW a more challenging technical procedure, compared to the more established and utilized chamber models currently used to study mammary tissue and fat pads 5-7. In addition, the ICW provides many challenges when optimizing the imaging.

A Novel High-resolution In vivo Imaging Technique to Study the Dynamic Response of Intracranial Structures to Tumor Growth and Therapeutics

We describe a novel in vivo imaging technique that couples fluorescent chimeric mice with intracranial windows and high-resolution 2-photon microscopy. This imaging platform aids studies of dynamic changes in brain tissue and microvasculature, at a single-cell level, following pathological insults and is adaptable to assess intracranial drug delivery and distribution.

소설 고해상도<em&gt; 생체 내</em종양의 성장과 치료학에 두개 내 구조물의 동적 응답을 공부하기&gt; 이미징 기술

We have successfully integrated previously established Intracranial window (ICW) technology 1-4 with intravital 2-photon confocal microscopy to develop a ...

미세유체역학(Microfluidics) 기반 혈관화된 인간 미세종양 모델 준비

Establishing a Physiologic Human Vascularized Micro-Tumor Model for Cancer Research

A lack of validated cancer models that recapitulate the tumor microenvironment of solid cancers in vitro remains a significant bottleneck for preclinical cancer research and therapeutic development.&#160;To overcome this problem, we have developed the vascularized microtumor (VMT), or tumor chip, a microphysiological system that realistically models the complex human tumor microenvironment. The VMT forms de novo within a microfluidic platform by co-culture of multiple human cell types under dynamic, physiological flow conditions. This tissue-engineered micro-tumor construct incorporates a living perfused vascular network that supports the growing tumor mass just as newly formed vessels do in vivo. Importantly, drugs and immune cells must cross the endothelial layer to reach the tumor, modeling in vivo physiological barriers to therapeutic delivery and efficacy. Since the VMT platform is optically transparent, high-resolution imaging of dynamic processes such as immune cell extravasation and metastasis can be achieved with direct visualization of fluorescently labeled cells within the tissue. Further, the VMT retains in vivo tumor heterogeneity, gene expression signatures, and drug responses. Virtually any tumor type can be adapted to the platform, and primary cells from fresh surgical tissues grow and respond to drug treatment in the VMT, paving the way toward truly personalized medicine. Here, the methods for establishing the VMT and utilizing it for oncology research are outlined. This innovative approach opens new possibilities for studying tumors and drug responses, providing researchers with a powerful tool to advance cancer research.

저자 스포트라이트: Creating Human Vascularized Micro-Tumors as Models for Translational Cancer Research (중개 암 연구를 위한 모델로서의 인간 혈관 미세 종양 생성) 

This protocol presents a physiologically relevant tumor-on-a-chip model to perform high-throughput basic and translational human cancer research, advancing drug screening, disease modeling, and personalized medicine approaches with a description of loading, maintenance, and evaluation procedures.

암 연구를 위한 생리학적 인간 혈관화 미세종양 모델 확립

A lack of validated cancer models that recapitulate the tumor microenvironment of solid cancers in vitro remains a significant bottleneck for preclinical ...

암 연구학

Guided Tumor Inoculation and Multimodal Imaging in Mice

A Dorsal Skinfold Window Chamber Tumor Mouse Model for Combined Intravital Microscopy and Magnetic Resonance Imaging in Translational Cancer Research

Preclinical intravital imaging such as microscopy and optical coherence tomography have proven to be valuable tools in cancer research for visualizing the tumor microenvironment and its response to therapy. These imaging modalities have micron-scale resolution but have limited use in the clinic due to their shallow penetration depth into tissue. More clinically applicable imaging modalities such as CT, MRI, and PET have much greater penetration depth but have comparatively lower spatial resolution (mm scale). 
To translate preclinical intravital imaging findings into the clinic, new methods must be developed to bridge this micro-to-macro resolution gap. Here we describe a dorsal skinfold window chamber tumor mouse model designed to enable preclinical intravital and clinically applicable (CT and MR) imaging in the same animal, and the image analysis platform that links these two disparate visualization methods. Importantly, the described window chamber approach enables the different imaging modalities to be co-registered in 3D using fiducial markers on the window chamber for direct spatial concordance. This model can be used for validation of existing clinical imaging methods, as well as for the development of new ones through direct correlation with "ground truth" high-resolution intravital findings. 
Finally, the tumor response to various treatments-chemotherapy, radiotherapy, photodynamic therapy-can be monitored longitudinally with this methodology using preclinical and clinically applicable imaging modalities. The dorsal skinfold window chamber tumor mouse model and imaging platforms described here can thus be used in a variety of cancer research studies, for example, in translating preclinical intravital microscopy findings to more clinically applicable imaging modalities such as CT or MRI.

저자 스포트라이트: 고해상도 생체 내 영상 및 MRI를 통합하여 정위 신체 방사선 치료 계획 향상

Translation of Intravital microscopy findings is challenged by its shallow depth penetration into tissue. Here we describe a dorsal window chamber mouse model that enables co-registration of intravital microscopy and clinically applicable imaging modalities (e.g., CT, MRI) for direct spatial correlation, potentially streamlining clinical translation of intravital microscopy findings.

중개 암 연구에서 생체 내 현미경 및 자기 공명 영상의 결합을 위한 Dorsal Skinfold Window Chamber 종양 마우스 모델

Preclinical intravital imaging such as microscopy and optical coherence tomography have proven to be valuable tools in cancer research for visualizing the ...

화학 요법에 의한 혈관 독성 - 실시간

요약

더 많은 비디오 탐색

MicroCT를 사용하여 대비 향상된 베셀 영상

유방암의 마우스 모델에서 종양 Microenvironment에 약물 응답의 영상을 라이브

라이브 마우스의 혈관 염증과 혈전 형성하는 동안 활성화 내피에 Heterotypic 혈소판 - 호중구 상호 작용의 실시간 영상

형광 공 초점 Videomicroscopy를 사용하여 종양 혈관의 생체 내 이미징

소설 고해상도

암 연구를 위한 생리학적 인간 혈관화 미세종양 모델 확립

암 연구를 위한 생리학적 인간 혈관화 미세종양 모델 확립

암 연구를 위한 생리학적 인간 혈관화 미세종양 모델 확립

중개 암 연구에서 생체 내 현미경 및 자기 공명 영상의 결합을 위한 Dorsal Skinfold Window Chamber 종양 마우스 모델

중개 암 연구에서 생체 내 현미경 및 자기 공명 영상의 결합을 위한 Dorsal Skinfold Window Chamber 종양 마우스 모델

화학 요법에 의한 혈관 독성 - 실시간

요약

더 많은 비디오 탐색

MicroCT를 사용하여 대비 향상된 베셀 영상

유방암의 마우스 모델에서 종양 Microenvironment에 약물 응답의 영상을 라​​이브

라이브 마우스의 혈관 염증과 혈전 형성하는 동안 활성화 내피에 Heterotypic 혈소판 - 호중구 상호 작용의 실시간 영상

형광 공 초점 Videomicroscopy를 사용하여 종양 혈관의 생체 내 이미징

소설 고해상도

암 연구를 위한 생리학적 인간 혈관화 미세종양 모델 확립

암 연구를 위한 생리학적 인간 혈관화 미세종양 모델 확립

암 연구를 위한 생리학적 인간 혈관화 미세종양 모델 확립

중개 암 연구에서 생체 내 현미경 및 자기 공명 영상의 결합을 위한 Dorsal Skinfold Window Chamber 종양 마우스 모델

중개 암 연구에서 생체 내 현미경 및 자기 공명 영상의 결합을 위한 Dorsal Skinfold Window Chamber 종양 마우스 모델

유방암의 마우스 모델에서 종양 Microenvironment에 약물 응답의 영상을 라이브