DNA Extraction from Experimental and Spiked Filters
6:02
Quantitative PCR Analysis
7:48
Results: Centrifugation Speed and Filter Load Influences the Measured Endotoxin Concentrations in Samples
8:35
Conclusion
Transkript
The overall goal of this procedure is to describe how to accurately analyze endotoxin and DNA content of extracted airborne particles sampled on filters. This method can help answer key questions in indoor and outdoor biological aerosol research,
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Two complementary analyses of atmospheric biological particles from air sampled filters are described herein: the extraction and detection of endotoxin, and of DNA.