Name: high throughput assay to examine egg-laying preferences of individual drosophila melanogaster
Uploaded: 2016-03-24T13:00:00.000+00:00
Duration: 3 min 58 s
Description: Watch this Scientific Journal Video about High Throughput Assay to Examine Egg-Laying Preferences of Individual Drosophila melanogaster at JoVE.com

We thank the Duke Physics Shop, especially Phil Lewis, for helping us build the behavioral apparatus and attachments and creating the drawing. This work is funded by the National Institutes of Health under award number R01GM100027.

Sinekler hazırlanması 1. tahlil edilecek <ol><li> Kültür standart pekmez uçar / 25 ° C ve% 65 nemde belirlenen inkübatör de Mısır unu medya. Şişeleri doldurmadık özen gösterin. Örneğin, dar bir gıda şişenin içine 8 kadın ve 6 erkek koydu. Not: Burada kullanılan &quot;ince gıda şişenin&quot; 2.3 cm&#39;lik bir iç çapa sahiptir. Biz genellikle her şişenin içine sinek gıda yaklaşık 10 ml dağıtılmıştır. Kullandığımız sinek yemek tarifi burada açıklanmıştır: http://flystocks.bio.indiana.edu/Fly_Work/media-recipes/molassesfood.html. </li><li> 2 - Şekil 1E gösterildiği gibi 3 gün kadın eclosion sonra, taze maya hamurlu şişeleri hazırlamak. % 0.5 propionik asit 10 ml aktif maya 6 g karıştırılarak yaş maya hamur yapın. Gıda şişeleri yanağında üzerine maya macun uygulamak için bir spatula kullanın. şişeleri içine 25 erkek - 20 35 eclosed kadın birlikte - 30 toplayın. <ol><li> Genel olarak, dişiler toplanır aynı flakonlarından erkekleri toplamak(Böylece zaman ve çaba kaydetmek için). Erkeklerin doğurganlık konusunda endişe var, ancak bunun yerine WT erkek kullanın. Ayrıca, maya macunu her gün taze hazırlayın nedenle yumurta üretimini stimüle etmek için önemlidir. </li></ol></li><li> Sinekler ısıya duyarlı olmadıkça, 25 ° C&#39;de,% 65 nemde toplanan kadın / erkek koruyun. </li><li> ~ 4 Sonra - 5 gün, kadın yumurtlama deneyler için hazır olup olmadığını görmek için şişeleri kontrol edin. Gıda ortamının yüzeyi aktif gıda burrowing larva ıslak olduğunda hazır olduklarında (- F Şekil 1E bakınız). Not: Gıda yüzeyi ıslak ve larvaları ile tarama hale geldiğinde Dişiler genellikle döşeme yumurta kesinti. Bu adım dişiler istenilen doku ile alt tabakaları karşılaştıklarında çok yumurtalarını bırakmak için hazır olacağını garanti (örn., ~% 1 agaroz). </li></ol> 2. Odası İnşaat, Montaj, Tahlil Kur <ol><li> (Bir atölye akrilik yumurtlama odaları inşa Fi varşekil 1A - D). Farklı parçaların mühendislik çizimi Ek Şekil 1&#39;de gösterilmiştir -. 3 Yüksek çözünürlüklü resimler de burada bulunabilir (http://www.rebeccayang.org/pdf/chamber%20design.pdf). </li><li> Şekil 1C&#39;de gösterildiği gibi, bölmenin yükleme (üst) parça halinde plastik levhalar yerleştirin. Yükleme bireysel yumurtlama arenalarda içine uçarken Bu alt yüzeyi olarak hizmet vermektedir. </li><li> CO 2 pad üzerinde kadın uyutmak ve her yumurtlama arenaya bunları tek tek yükleyin. Sinekler CO 2 kurtarmak ve yeni ortama acclimated hale için ~ 30 dakika bekleyin. </li><li> agaroz substratlar hazırlayın. <ol><li> kolaylık sağlamak için, 55 ° C su banyosunda eritilir% 1 agaroz bir premade şişe tutmak. </li><li> 50 ml konik bir tüp içine hazır sükroz solüsyonu (2 M) istenilen miktarda ilave edin ve agaroz uygun miktarda ile karıştırılması. Örneğin, 150 mM sükroz alt-tabaka, pla hazırlamakce 750 tüp içine 2 M sukroz çözeltisi ul ve ardından 10 mi işaretine kadar agaroz ile tüp doldurun. </li><li> aynı şekilde, düz alt-tabakanın hazırlanması yerine sükroz solüsyonu damıtılmış su ilave edilir. Not: Bu protokol agaroz nihai konsantrasyon az% 1 &#39;den daha azdır. % 1,1 ve iki yüzeylerde aynı agaroz konsantrasyonu olduğu - o ~ 0.9 içinde olacak şekilde kontrol edilir olarak bizim deneyim, agaroz tam konsantrasyonu çok uzun fark etmez. </li></ol></li><li> Şekil 1D görüldüğü gibi her bir çukur içine odası ve pipet agaroz substrat 1000 ul substrat (alt) parça al. </li><li> agaroz ~ 30 dakika boyunca katılaşmaya izin verin. </li><li> agaroz yüzeyler ve sinekler hazır olduğunda, yumurtlama odasının her üç parçalarını birleştirmek ve ardından plastik levhalar çıkar. </li><li> sinek kuluçka odaları yerleştirin. Not: yumurtlama deney uzunluğu deneyimlerini bağlı olarak değişebilirzca ihtiyacı var. (- 16 saat 14), tipik olarak deney O / N çalıştırın. Ayrıca, yumurtlama tercihleri ​​sirkadiyen zamanlama önemli etkisi gözlenmemiştir. </li><li> Odasına CO2 enjekte kadın anestezisi. , Odasına sökün sinek morguna içine anestezi sinekler atın (yani., Boş kahve, bazı mısır yağı ile dolu olabilir). Kayıt tutulması sonuçların fotoğraflarını çekmek (bakınız Şekil 2). </li><li> elle yumurta sayısını ve analiz için tercih endekslerini hesaplamak. Olarak tercih indeksi hesaplamak (N - N b) / (N + N b) N ve N b sırasıyla sitede vs. sitesi b yumurta sayısını temsil eder. </li></ol>

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The authors have nothing to disclose.

odaları ve burada açıklanan protokoller önceki yumurtlama deneyleri üzerinde çeşitli geliştirmeler var. Birincisi, önemli ölçüde tek hayvan tercihlerini analiz etmek ve verimi arttırmak. Her oda 30 tek kadın tahlil edebilir ve bir saat kurmak için daha az sürer. İkincisi, önceki yöntemlere göre yumurtlama tercihleri ​​tutarlılığını artırmak. arena boyutlarının standardizasyon, yüzeyler arasındaki yumurtlama substratların boyutu ve mesafe daha kolay farklı deneysel gün arasında ve fa...

Drosophila melanogaster is a powerful genetic model organism to study the neural basis of behaviors. The rapid developments of genetic tools to manipulate neurons in a targeted manner and the emergence of sophisticated behavioral analysis tools have significantly improved our ability to dissect the circuit mechanisms that underlie the sensory-motor transformation processes of several innate and learned behaviors1-3.
Drosophila egg-laying is a suitable model to study the neural basis of simple decision making processes. In particular, Drosophila females have been shown to possess the ability to compare and rank their options before "committing" to depositing an egg onto a given option4-8. For example, when given only a plain (sucrose-free) substrate or only a sucrose-containing substrate, females readily accept either option for egg-laying. However, when presented with both options, females robust reject the sucrose substrate in some contexts7,9,10. Relatively little is known about the neural mechanisms that allow females to "choose the greater of two goods", however. A major obstacle has been the lack of an efficient method to assay egg-laying preferences such that one can use a systematic genetic screening approach to study this problem.
In this report, we describe the protocol we developed that allows egg-laying preferences of females to be assayed at single-animal resolution and with substantially improved throughput and consistency over previous methods. Specifically, we provide the blueprints for constructing the chambers we designed, the protocol for preparing the females so that each is primed to lay many eggs, and the protocol for using the chambers.

<table><tbody><tr><td>UltraPure Agarose&amp;nbsp;</td><td>Invitrogen</td><td>16500-500</td><td></td></tr><tr><td>Sucrose</td><td>Sigma</td><td>S0389</td><td></td></tr><tr><td>Water bath&amp;nbsp;</td><td>Fisher</td><td>15-462-6Q</td><td></td></tr><tr><td>LifeCam Cinema webcam</td><td>Microsoft</td><td>H5D-00013</td><td></td></tr><tr><td>Red LEDs</td><td>Cree</td><td>C503B-RAN-CA0B0AA1</td><td></td></tr><tr><td>Egg-laying chambers</td><td>Custom Built</td><td></td><td></td></tr><tr><td>Camera holders</td><td>Custom Built</td><td></td><td></td></tr><tr><td>LED holders</td><td>Custom Built</td><td></td><td></td></tr><tr><td>Fly vials (narrow)</td><td>Genesee</td><td>32-116BC</td><td></td></tr></tbody></table>

high throughput assay to examine egg-laying preferences of individual drosophila melanogaster

Son zamanlarda, Drosophila yumurtlama tercihi basit karar verme süreçlerine nöral temelini incelemek için bir genetik uysal bir model olarak ortaya çıkmıştır. yumurtalarını yatırmak siteleri seçerken, erkek sineklerin kendi seçenekleri göreceli çekiciliği sıralama ve seçme yeteneğine sahiptir &quot;daha büyük iki mal.&quot; bir de toplum tabanlı ve kurmak için zahmetli olduğu gibi, bu basit karar verme sürecini altında yatan devre bazında aramak için sistematik bir genetik tarama yaklaşım almak istiyorsa Ancak, çoğu yumurtlama tercih deneyleri pratik değildir. Tek kadın yumurtlama tercihlerini okuyan verimini arttırmak için, biz en fazla otuz bireyin her can aynı anda tahlil yumurtlama tercihleri ​​her kadın yüksek yumurtlama oranına sahip sağlayan bir protokol yanı sıra uçar özel odalarını geliştirdi (yani onların tercihi kolayca ayırt edilebilir ve daha inandırıcı olduğunu). Yaklaşımımız yürütmek kolaydırve çok tutarlı sonuçlar üretir. Ayrıca, bu odalar, video yumurtlama hayvanların kayıt ve optogenetics çalışmaları için ışık sunmak için izin farklı ekleri ile donatılmış olabilir. Bu makalede, bu odalar içinde denenecek olan sinekler hazırlanması için, bu odalar ve prosedür imal edilmesi için planları içerir.

Yumurtlama odaları birçok parçadan oluşur: bir alt tabaka (alt) parçası, bir bölücü (orta) parçası, bir yükleme (üst) parça ve 2 sürgülü kapı (Şekil 1A - D). Bu parçalar yumurtlama deneyler daha önce bağımsız kurulum sinekler ve yüzeyler için kullanılır. Şekil 1F dişi sineklerin yumurtlama için hazır olduğunda şişeler nasıl görünmelidir gösterir. Sinekler düz alt-tabaka ve bir sükroz içeren alt-tabaka arasında b...

Watch this Scientific Journal Video about High Throughput Assay to Examine Egg-Laying Preferences of Individual Drosophila melanogaster at JoVE.com

High Throughput Assay to Examine Egg-Laying Preferences of Individual Drosophila melanogaster

Yüksek Verimli Testi Bireyin Yumurta Yumurtlayan Tercihler incelemek için<em&gt; Drosophila melanogaster</em

This protocol describes a high throughput assay for testing egg-laying preferences of Drosophila melanogaster at single-animal resolution. This assay provides a simple, efficient, and scalable platform to identify genes and circuit components that control a simple decision-making process.

Recently, egg-laying preference of Drosophila has emerged as a genetically tractable model to study the neural basis of simple decision-making processes. ...

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Research

JoVE Journal

Neuroscience

High Throughput Assay to Examine Egg-Laying Preferences of Individual Drosophila melanogaster

7.9K Görüntüleme.  Duke University. This protocol describes a high throughput assay for testing egg-laying preferences of Drosophila melanogaster at single-animal resolution. This assay provides a simple, efficient, and scalable platform to identify genes and circuit components that control a simple decision-making process.

Video: High Throughput Assay to Examine Egg-Laying Preferences of Individual Drosophila melanogaster

Methods to Assay Drosophila Behavior

Drosophila melanogaster, the fruit fly, has been used to study molecular mechanisms of a wide range of human diseases such as cancer, cardiovascular disease and various neurological diseases1. We have optimized simple and robust behavioral assays for determining larval locomotion, adult climbing ability (RING assay), and courtship behaviors of Drosophila. These behavioral assays are widely applicable for studying the role of genetic and environmental factors on fly behavior. Larval crawling ability can be reliably used for determining early stage changes in the crawling abilities of Drosophila larvae and also for examining effect of drugs or human disease genes (in transgenic flies) on their locomotion. The larval crawling assay becomes more applicable if expression or abolition of a gene causes lethality in pupal or adult stages, as these flies do not survive to adulthood where they otherwise could be assessed. This basic assay can also be used in conjunction with bright light or stress to examine additional behavioral responses in Drosophila larvae. Courtship behavior has been widely used to investigate genetic basis of sexual behavior, and can also be used to examine activity and coordination, as well as learning and memory. Drosophila courtship behavior involves the exchange of various sensory stimuli including visual, auditory, and chemosensory signals between males and females that lead to a complex series of well characterized motor behaviors culminating in successful copulation. Traditional adult climbing assays (negative geotaxis) are tedious, labor intensive, and time consuming, with significant variation between different trials2-4. The rapid iterative negative geotaxis (RING) assay5 has many advantages over more widely employed protocols, providing a reproducible, sensitive, and high throughput approach to quantify adult locomotor and negative geotaxis behaviors. In the RING assay, several genotypes or drug treatments can be tested simultaneously using large number of animals, with the high-throughput approach making it more amenable for screening experiments.

Methods to Assay Drosophila Behavior

Drosophila melanogaster is a genetically and behaviorally tractable model system that has been used to understand the molecular and cellular basis of many important biological processes for over a century 1. Drosophila has been well exploited to gain insights into the genetic basis of fly behavior.

Test için Yöntemler Drosophila Davranış

Drosophila melanogaster, the fruit fly, has been used to study molecular mechanisms of a wide range of human diseases such as cancer, cardiovascular ...

Nörobilim

Light Preference Assay to Study Innate and Circadian Regulated Photobehavior in Drosophila Larvae

Light acts as environmental signal to control animal behavior at various levels. The Drosophila larval nervous system is used as a unique model to answer basic questions on how light information is processed and shared between rapid and circadian behaviors. Drosophila larvae display a stereotypical avoidance behavior when exposed to light. To investigate light dependent behaviors comparably simple light-dark preference tests can be applied. In vertebrates and arthropods the neural pathways involved in sensing and processing visual inputs partially overlap with those processing photic circadian information. The fascinating question of how the light sensing system and the circadian system interact to keep behavioral outputs coordinated remains largely unexplored. Drosophila is an impacting biological model to approach these questions, due to a small number of neurons in the brain and the availability of genetic tools for neuronal manipulation. The presented light-dark preference assay allows the investigation of a range of visual behaviors including circadian control of phototaxis.

Light Preference Assay to Study Innate and Circadian Regulated Photobehavior in Drosophila Larvae

Here we describe a light-dark preference test for Drosophila larva. This assay provides information about innate and circadian regulation of light sensing and processing photobehavior.

Içinde Doğuştan gelen ve sirkadiyen Ayarlı Photobehavior Eğitim ışık tercih Testi<em&gt; Drosophila</em&gt; Larva

Light acts as environmental signal to control animal behavior at various levels. The Drosophila larval nervous system is used as a unique model to answer ...

A Single-fly Assay for Foraging Behavior in Drosophila

For many animals, hunger promotes changes in the olfactory system in a manner that facilitates the search for appropriate food sources. In this video article, we describe an automated assay to measure the effect of hunger or satiety on olfactory dependent food search behavior in the adult fruit fly Drosophila melanogaster. In a light-tight box illuminated by red light that is invisible to fruit flies, a camera linked to custom data acquisition software monitors the position of six flies simultaneously. Each fly is confined to walk in individual arenas containing a food odor at the center. The testing arenas rest on a porous floor that functions to prevent odor accumulation. Latency to locate the odor source, a metric that reflects olfactory sensitivity under different physiological states, is determined by software analysis. Here, we discuss the critical mechanics of running this behavioral paradigm and cover specific issues regarding fly loading, odor contamination, assay temperature, data quality, and statistical analysis.

A Single-fly Assay for Foraging Behavior in Drosophila

In this video article, we describe an automated assay to measure the effect of hunger or satiety on olfactory dependent food search behavior in the adult fruit fly Drosophila melanogaster.

Toplayıcılık Davranış için Tek-sinek Deneyi<em&gt; Drosophila</em

For many animals, hunger promotes changes in the olfactory system in a manner that facilitates the search for appropriate food sources. In this video ...

Assay for Blood-brain Barrier Integrity in Drosophila melanogaster

Proper nervous system development includes the formation of the blood-brain barrier, the diffusion barrier that tightly regulates access to the nervous system and protects neural tissue from toxins and pathogens. Defects in the formation of this barrier have been correlated with neuropathies, and the breakdown of this barrier has been observed in many neurodegenerative diseases. Therefore, it is critical to identify the genes that regulate the formation and maintenance of the blood-brain barrier to identify potential therapeutic targets. In order to understand the exact roles these genes play in neural development, it is necessary to assay the effects of altered gene expression on the integrity of the blood-brain barrier. Many of the molecules that function in the establishment of the blood-brain barrier have been found to be conserved across eukaryotic species, including the fruit fly, Drosophila melanogaster. Fruit flies have proven to be an excellent model system for examining the molecular mechanisms regulating nervous system development and function. This protocol describes a step-by-step procedure to assay for blood-brain barrier integrity during the embryonic and larval stages of D. melanogaster development.

Assay for Blood-brain Barrier Integrity in Drosophila melanogaster

Blood-brain barrier integrity is critical for nervous system function. In Drosophila melanogaster, the blood-brain barrier is formed by glial cells during late embryogenesis. This protocol describes methods to assay for blood-brain barrier formation and maintenance in D. melanogaster embryos and third instar larvae.

Drosophila melanogaster Kan-beyin Bariyer Bütünlüğü için Tahse

Proper nervous system development includes the formation of the blood-brain barrier, the diffusion barrier that tightly regulates access to the nervous ...

Gelişim Biyolojisi

A Behavioral Assay for Mechanosensation of MARCM-based Clones in Drosophila melanogaster

Because of the structural and functional homology to the hair cells of the mammalian inner ear, the neurons that innervate the Drosophila external sense organs provide an excellent model system for the study of mechanosensation. This protocol describes a simple touch behavior in fruit flies which can be used to identify mutations that interfere with mechanosensation. The tactile stimulation of a macrochaete bristle on the thorax of flies elicits a grooming reflex from either the first or third leg. Mutations that interfere with mechanotransduction (such as NOMPC), or with other aspects of the reflex arc, can inhibit the grooming response. A traditional screen of adult behaviors would have missed mutants that have essential roles during development. Instead, this protocol combines the touch screen with mosaic analysis with a repressible cell marker (MARCM) to allow for only limited regions of homozygous mutant cells to be generated and marked by the expression of green fluorescent protein (GFP). By testing MARCM clones for abnormal behavioral responses, it is possible to screen a collection of lethal p-element mutations to search for new genes involved in mechanosensation that would have been missed by more traditional methods.

A Behavioral Assay for Mechanosensation of MARCM-based Clones in Drosophila melanogaster

In order to identify novel mutations affecting mechanosensation, we designed an assay that measures the behavioral response to tactile stimulation of fly bristles in mutant clones generated by the MARCM method. The combination of techniques allows for the identification of mechanosensitive mutations that would otherwise be lethal.

Arasında Mechanosensation için bir davranış tahlilinde klonlarda MARCM tabanlı<em&gt; Drosophila melanogaster</em

Because of the structural and functional homology to the hair cells of the mammalian inner ear, the neurons that innervate the Drosophila external sense ...

Measuring and Altering Mating Drive in Male Drosophila melanogaster

Despite decades of investigation, the neuronal and molecular bases of motivational states remain mysterious. We have recently developed a novel, reductionist, and scalable system for in-depth investigation of motivation using the mating drive of male Drosophila&#160;melanogaster (Drosophila), the methods for which we detail here. The behavioral paradigm centers on the finding that male mating drive decreases alongside fertility over the course of repeated copulations and recovers over ~3 d. In this system, the powerful neurogenetic tools available in the fly converge with the genetic accessibility and putative wiring diagram available for sexual behavior. This convergence allows rapid isolation and interrogation of small neuronal populations with specific motivational functions. Here we detail the design and execution of the satiety assay that is used to measure and alter courtship motivation in the male fly. Using this assay, we also demonstrate that low male mating drive can be overcome by stimulating dopaminergic neurons. The satiety assay is simple, affordable, and robust to influences of genetic background. We expect the satiety assay to generate many new insights into the neurobiology of motivational states.

Measuring and Altering Mating Drive in Male Drosophila melanogaster

This article describes a behavioral assay that uses male mating drive in Drosophila melanogaster to study motivation. Using this method, researchers can utilize advanced fly neurogenetic techniques to uncover the genetic, molecular, and cellular mechanisms that underlie this motivation.

Ölçme ve Male Değiştirme Çiftleşme Sürücü<em&gt; Drosophila melanogaster</em

Despite decades of investigation, the neuronal and molecular bases of motivational states remain mysterious. We have recently developed a novel, ...

A Temperature Gradient Assay to Determine Thermal Preferences of Drosophila Larvae

Many animals, including the fruit fly, Drosophila melanogaster, are capable of discriminating minute differences in&#160;environmental temperature, which enables them to seek out their preferred thermal landscape. To define the temperature preferences of larvae over a defined linear range, we developed an assay using a temperature gradient. To establish a single-directional gradient, two aluminum blocks are connected to independent water baths, each of which controls the temperature of individual blocks. The two blocks set the lower and upper limits of the gradient. The temperature gradient is established by placing an agarose-coated aluminum plate over the two water-controlled blocks so that the plate spans the distance between them. The ends of the aluminum plate that is set on the top of the water blocks defines the minimum and maximum temperatures, and the regions in-between the two blocks form a linear temperature gradient. The gradient assay can be applied to&#160;larvae of different ages and can be used to identify mutants that exhibit phenotypes, such as those with mutations affecting genes encoding TRP channels and opsins, which are required for temperature discrimination.

A Temperature Gradient Assay to Determine Thermal Preferences of Drosophila Larvae

Here, we present a protocol to determine the preferred environmental temperature of Drosophila larvae using a continuous thermal gradient.

Drosophila larva termal tercihlerini belirlemek için bir sıcaklık gradyanı tahlil

Many animals, including the fruit fly, Drosophila melanogaster, are capable of discriminating minute differences in&#160;environmental temperature, which ...

A Rapid Food-Preference Assay in Drosophila

To select food with nutritional value while avoiding the consumption of harmful agents, animals need a sophisticated and robust taste system to evaluate their food environment. The fruit fly, Drosophila melanogaster, is a genetically tractable model organism that is widely used to decipher the molecular, cellular, and neural underpinnings of food preference. To analyze fly food preference, a robust feeding method is needed. Described here is a two-choice feeding assay, which is rigorous, cost-saving, and fast. The assay is Petri-dish-based and involves the addition of two different foods supplemented with blue or red dye to the two halves of the dish. Then, ~70 prestarved, 2-4-day-old flies are placed in the dish and&#160;allowed to choose between blue and red foods in the dark for about 90 min. Examination of the abdomen of each fly is followed by the calculation of the preference index. In contrast to multiwell plates, each Petri dish takes only ~20 s to fill and saves time and effort. This feeding assay can be employed to quickly determine whether flies like or dislike a particular food.

A Rapid Food-Preference Assay in Drosophila

We present a protocol for a two-choice feeding assay for flies. This feeding assay is fast and easy to run and is suitable not only for small-scale laboratory research, but also for high-throughput behavioral screens in flies.

Drosophila&apos;da Hızlı Bir Yemek Tercihi Tahlil

To select food with nutritional value while avoiding the consumption of harmful agents, animals need a sophisticated and robust taste system to evaluate ...

Egg Laying Assay: A Method to Quantify the Egg-Laying Behavior of C. elegans

Source: Lee, S. Y. and Kang, K. <a href="https://www.jove.com/video/60419/measuring-effects-bacteria-chemicals-on-intestinal-permeability">Measuring the Effect of Chemicals on the Growth and Reproduction of&#160;Caenorhabditis elegans</a>.&#160;J. Vis. Exp. (2017).
C. elegans egg-laying behavior can provide important information in developmental, reproductive, and anatomical studies.&#160; This video introduces the important steps of an egg laying assay and shows a sample experiment in which a drug is tested for reproductive toxicity.

Yumurtlama Deneyi: C. elegans'ın Yumurtlama Davranışını Ölçmek İçin Bir Yöntem

Source: Lee, S. Y. and Kang, K. Measuring the Effect of Chemicals on the Growth and Reproduction of&#160;Caenorhabditis elegans.&#160;J. Vis. Exp. 
...

JoVE Encyclopedia of Experiments

Biyoloji

Caenorhabditis elegans (solucan)

Temel Yöntemler

Island Assay: A High-Throughput Method to Evaluate Drosophila Locomotor Behavior

Source: Eidhof, I., et al. <a href="https://www.jove.com/video/55892/" target="_blank">High-throughput Analysis of Locomotor Behavior in the Drosophila Island Assay</a>. J. Vis. Exp. (2017).
This video describes the island assay, a behavioral method used to study Drosophila locomotion. The sample protocol features a setup for the assay compatible with semi-automated image analysis.

Ada Testi: Drosophila Lokomotor Davranışını Değerlendirmek İçin Yüksek Verimli Bir Yöntem

Source: Eidhof, I., et al. High-throughput Analysis of Locomotor Behavior in the Drosophila Island Assay. J. Vis. Exp. (2017).
This video describes the ...

Yüksek Verimli Testi Bireyin Yumurta Yumurtlayan Tercihler incelemek için

Özet

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Test için Yöntemler Drosophila Davranış

Içinde Doğuştan gelen ve sirkadiyen Ayarlı Photobehavior Eğitim ışık tercih Testi

Toplayıcılık Davranış için Tek-sinek Deneyi

Drosophila melanogaster Kan-beyin Bariyer Bütünlüğü için Tahse

Arasında Mechanosensation için bir davranış tahlilinde klonlarda MARCM tabanlı

Ölçme ve Male Değiştirme Çiftleşme Sürücü

Drosophila larva termal tercihlerini belirlemek için bir sıcaklık gradyanı tahlil

Drosophila'da Hızlı Bir Yemek Tercihi Tahlil

Yumurtlama Deneyi: C. elegans'ın Yumurtlama Davranışını Ölçmek İçin Bir Yöntem

Ada Testi: Drosophila Lokomotor Davranışını Değerlendirmek İçin Yüksek Verimli Bir Yöntem