Staged Embryo Acquisition and Single Cell Dissociation
5:03
Microfluidic Chip Cell Loading
6:55
Results: Representative Single Zebrafish Nkx2.5ZsY Positive Cell Analysis
8:16
Conclusion
Transkript
The overall goal of this experiment is to isolate single cells from early stage zebrafish embryos for their capture in a microfluidic-based single-cell multiplex system to assess the gene expression of individual cells. This method can help answer
Bu içeriğe erişmek için giriş yapın veya ücretsiz denemenizi başlatın
This protocol describes a method for isolating single cells from zebrafish embryos, enriching for cells of interest, capturing zebrafish cells in microfluidic based single cell multiplex systems, and assessing gene expression from single cells.