Assessing VSMC Calcification Using the Von Kossa Staining Method
6:43
Results: MGP Deficiency Results in Vascular Calcification in the Absence of Vascular Inflammation and MGP Protects Cultured VSMCs from Calcification
8:03
Conclusion
Transkript
The overall goal of this procedure is to visualize aortic vascular calcification ex vivo and to model in vivo vascular calcification using cultured primary murine aortic smooth muscle cells. This method can help answer key questions in the field o
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Vascular calcification is an important predictor of and contributor to human cardiovascular disease. This protocol describes methods for inducing calcification of cultured primary vascular smooth muscle cells and for quantifying calcification and macrophage burden in animal aortas using near-infrared fluorescence imaging.