Calcification of Vascular Smooth Muscle Cells and Imaging of Aortic Calcification and Inflammation
19.5K Views
•
08:43 min
•
May 31st, 2016
DOI :
May 31st, 2016
•Caitlin O'Rourke*1, Georgia Shelton*1,2, Joshua D. Hutcheson3,4, Megan F. Burke2, Trejeeve Martyn1, Timothy E. Thayer2, Hannah R. Shakartzi1, Mary D. Buswell1, Robert E. Tainsh1, Binglan Yu1,4, Aranya Bagchi1,4, David K. Rhee2,4, Connie Wu1,2,4, Matthias Derwall5, Emmanuel S. Buys1,4, Paul B. Yu3,4, Kenneth D. Bloch1,2,4, Elena Aikawa3,4, Donald B. Bloch1,5,6, Rajeev Malhotra2,4
1Anesthesia Center for Critical Care Research of the Department of Anesthesia, Critical Care, and Pain Medicine, Massachusetts General Hospital, 2Cardiovascular Research Center and Cardiology Division of the Department of Medicine, Massachusetts General Hospital, 3Cardiovascular Division, Brigham and Women's Hospital, 4Harvard Medical School, 5Department of Anesthesiology, Uniklinik RWTH Aachen, RWTH Aachen University, 6Center for Immunology and Inflammatory Diseases and the Division of Rheumatology, Allergy, and Immunology of the Department of Medicine, Massachusetts General Hospital
Chapters in this video
0:05
Title
0:58
Tail Vein Injection
1:35
Mouse Dissection
3:39
Isolation of Primary Murine Aortic VSMCs
5:21
Assessing VSMC Calcification Using the Von Kossa Staining Method
6:43
Results: MGP Deficiency Results in Vascular Calcification in the Absence of Vascular Inflammation and MGP Protects Cultured VSMCs from Calcification
8:03
Conclusion
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