Calcification of Vascular Smooth Muscle Cells and Imaging of Aortic Calcification and Inflammation

19.4K Views

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08:43 min

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May 31st, 2016

DOI :

10.3791/54017-v

May 31st, 2016

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¹Anesthesia Center for Critical Care Research of the Department of Anesthesia, Critical Care, and Pain Medicine, Massachusetts General Hospital, ²Cardiovascular Research Center and Cardiology Division of the Department of Medicine, Massachusetts General Hospital, ³Cardiovascular Division, Brigham and Women's Hospital, ⁴Harvard Medical School, ⁵Department of Anesthesiology, Uniklinik RWTH Aachen, RWTH Aachen University, ⁶Center for Immunology and Inflammatory Diseases and the Division of Rheumatology, Allergy, and Immunology of the Department of Medicine, Massachusetts General Hospital

Summary

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Keywords Vascular Calcification

Aortic Calcification

Vascular Smooth Muscle Cells

Chapters in this video

0:05

Title

0:58

Tail Vein Injection

1:35

Mouse Dissection

3:39

Isolation of Primary Murine Aortic VSMCs

5:21

Assessing VSMC Calcification Using the Von Kossa Staining Method

6:43

Results: MGP Deficiency Results in Vascular Calcification in the Absence of Vascular Inflammation and MGP Protects Cultured VSMCs from Calcification

8:03

Conclusion

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