Sectioning the Paraffin-embedded Mammary Tissue on the Microtome and Automated H and E Staining
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Results: Lesion Identification in Whole Mount Mammary Gland Sections
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Conclusion
Transkript
The overall goal of this procedure is to create high-quality stained sections from whole mounted mouse mammary tissue for lesion identification at the microscopic level. This method can help answer key questions involving mammary gland evaluation
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We developed a method to successfully remove, process, section, and stain, for histopathological evaluation, mammary tissue that had originally been fixed on slides as whole mounts. This method may promote the collection and evaluation of mammary gland whole mounts in reproductive and developmental test guideline studies.