Collecting NPCs for Plating of Experimental Conditions
2:30
Neural Precursor Cell DNA Synthesis Assay
3:47
S Phase Entry Assay
4:39
Neural Precursor Cell Number Assay
5:41
Neural Precursor Cell Neurosphere Migration Assay
7:58
Results: Use of Phase-contrast and Fluorescence Microscopy to Study the Proliferation, Migration, and Neurite Outgrowth of Neural Precursor Cells
9:51
Conclusion
Transkript
The overall goal of this protocol is to characterize early brain developmental processes of human neural precursor cells in a rapid and reproducible fashion, and to define the effects of important extracellular factors in regulating these processe
Bu içeriğe erişmek için giriş yapın veya ücretsiz denemenizi başlatın
Neurodevelopmental processes such as proliferation, migration, and neurite outgrowth are often perturbed in neuropsychiatric diseases. Thus, we present protocols to rapidly and reproducibly assess these neurodevelopmental processes in human iPSC-derived NPCs. These protocols also allow the assessment of the effects of relevant growth factors and therapeutics on NPC development.